Candidate genes that have facilitated freshwater adaptation by palaemonid prawns in the genus : identification and expression validation in a model species ().

Background: The endemic Australian freshwater prawn, , provides a model for exploring genes involved with freshwater adaptation because it is one of the relatively few species that can complete its entire life cycle in freshwater.

Methods: The present study was conducted to identify potential candidate genes that are likely to contribute to effective freshwater adaptation by using a transcriptomics approach. assembly of 75 bp paired end 227,564,643 high quality Illumina raw reads from 6 different cDNA libraries revealed 125,917 contigs of variable lengths (200-18,050 bp) with an N50 value of 1597.

Comparative analysis of gill transcriptomes of two freshwater crayfish, Cherax cainii and C. destructor.

We undertook deep sequencing of gill transcriptomes from two freshwater crayfish, Cherax cainii and Cherax destructor, in order to generate genomic resources for future genomics research. Over 83 and 100 million high quality (quality score (Q)≥30) paired-end Illumina reads (150 bp) were assembled into 147,101 and 136,622 contigs in C. cainii and C.

Assembly and annotation of a non-model gastropod (Nerita melanotragus) transcriptome: a comparison of de novo assemblers.

Background: The sequencing, de novo assembly and annotation of transcriptome datasets generated with next generation sequencing (NGS) has enabled biologists to answer genomic questions in non-model species with unprecedented ease. Reliable and accurate de novo assembly and annotation of transcriptomes, however, is a critically important step for transcriptome assemblies generated from short read sequences. Typical benchmarks for assembly and annotation reliability have been performed with model species.