A green impedimetric aptasensor for non-invasive and high-selective detection of tramadol validated by molecular dynamic simulation.

Although the dosage controlling of tramadol (TRA) as a banned deadly drug in human biofluids is medicolegally important a biocompatible method for its high-selective detection with fewer false interferences has been scarcely reported. Herein, a new impedimetric aptasensor is introduced by utilizing the aptamer (Apt) sequence with high affinity to TRA for the first time to non-invasively measure it. An oriented nanolayer of Au nanoparticles (AuNPs) is easily formed on the surface by the electrodeposition technique to high-densely load the Apt and embed the novel aptasensing interface via a user-friendly methodology.

Applicability of a green nanocomposite consists of reduced graphene oxide and β-cyclodextrin for electrochemical tracing of methadone in human biofluids validated by international greenness indexes.

Background: Detecting methadone (MET) is crucial due to its severe side effects.

Method: Herein, a green nanocomposite based on reduced graphene (rGO) and β-cyclodextrin (β-CD) has been introduced to modify a glassy carbon electrode (GCE) for real-time measurement of MET. This eco-friendly sensing interface has synergistically benefited from both advantages of rGO and β-CD including excellent electron transfer tunneling and surface area enhancement to selectively trap MET based on its shape and size.

High-performance cellulose acetate fibers-loaded Alca layered double oxide adsorbents towards efficient elimination of anionic pollutants: Mechanism adsorption and RSM-CCD approach.

In the present research, we investigate Congo red (CR) removal by layered double hydroxide and oxide AlCa on cellulose acetate (CA) fiber as anion-adsorbents in aqueous solution. The as-prepared composite was characterized by FE-SEM, XRD, FTIR, EDS-mapping and BET-BJH analyses. The CR adsorption ability on AlCa LDH/CA and AlCa LDO/CA adsorbents was evaluated.

Central composite design-assisted visual and non-invasive detection of sertraline by sweet lemon waste-derived core-shell AuNPs@CDs.

This study reports a fast and visual detection method of antidepressant sertraline (SRT) drug by the core-shell AuNPs@CDs as the nanoprobes. The CDs has been eco-friendly synthesized from sweet lemon wastes to directly reduce Au to AuNPs without any external photoirradiation process or additional reductants. Optimizing key variables that impact the sensing process has been done using the central composite design (CCD) approach to simulate the assay condition before the analysis.

Passive Infrared Sensor-Based Occupancy Monitoring in Smart Buildings: A Review of Methodologies and Machine Learning Approaches.

Buildings are rapidly becoming more digitized, largely due to developments in the internet of things (IoT). This provides both opportunities and challenges. One of the central challenges in the process of digitizing buildings is the ability to monitor these buildings' status effectively.

A smartphone-based colorimetric assay using Cu-tannic acid nanosheets (Cu-TA NShs) as a laccase-mimicking nanozyme for visual detection of quercetin in vegetables.

The interaction of Cu-tannic acid nanosheets (Cu-TA NShs) as nanozyme in a surfactant solution of CTAB under relatively acidic conditions is shown to exhibit a catalytic effect on quercetin (Qur). This catalytic property of Cu-TA NShs, which mimics laccase enzyme with many advantages, has been applied to developing a selective colorimetric sensor for the determination of trace amounts of Qur in vegetable samples. This strategy presents a desirable linear relationship between the absorbance signal intensity and the concentrations of Qur from 0.

Long-term outcomes of the classic Konno-Rastan procedure in paediatric and adult patients: impact of aortic annulus size on patient outcomes.

Objectives: The classic Konno-Rastan procedure may yield different outcomes regarding aortic annulus diameters ≤15 mm and larger. Focusing on the effect of the diameter of the aortic annulus, we described the long-term outcomes of our patients.

Methods: The outcomes of paediatric and adult patients who underwent surgery from 2000 to 2021 were studied retrospectively.

Development of dispersive liquid-liquid microextraction with solid-phase evaporation as a novel hyphenated method prior to ion mobility spectrometry and its application for trace analysis of fluoxetine.

For the hyphenating of dispersive liquid-liquid microextraction (DLLME) with nano mesoporous solid-phase evaporation (SPE) as a novel method, MCM-41@SiO was synthesized and used as a nano mesoporous adsorbent for coating on the solid-phase fiber, preconcentration of fluoxetine antidepressant drug (as a model compound), and total evaporation of the extraction solvents obtained by the DLLME method. To detect the analyte molecules, a corona discharge ionization-ion mobility spectrometer (CD-IMS) was applied. In order to increase the extraction efficiency and the IMS signal of the fluoxetine drug, some variables including extraction solvent and its volume, disperser solvents and its volume, sample solution pH, desorption temperature, and evaporation time of the solvent from the solid-phase fiber were chosen and optimized.

Copper-Guanosine Nanorods (Cu-Guo NRs) as a Laccase Mimicking Nanozyme for Colorimetric Detection of Rutin.

Inspired by laccase activity, herein, Cu-guanosine nanorods (Cu-Guo NRs) have been synthesized for the first time through a simple procedure. The activity of the Cu-Guo NR as the laccase mimicking nanozyme has been examined in the colorimetric sensing of rutin (Rtn) by a novel and simple spectrophotometric method. The distinct changes in the absorbance signal intensity of Rtn and a distinguished red shift under the optimum condition based on pH and ionic strength values confirmed the formation of the oxidized form of Rtn (o-quinone) via laccase-like nanozyme activity of Cu-Guo NRs.

A new microfluidic-chip device followed by sensitive image analysis of smart phone for simultaneous determination of dyes with different acidic-basic properties.

In this study, a new microfluidic-chip coupled with micro solid phase extraction (μ-SPE) and a RGB detection system was designed. The method was used for extraction and simultaneous determination of trace amounts of dyes with different acidic-basic properties. Erythrosine (Ery) and Crystal Violet (CV) were selected as acidic and basic model analytes, respectively.

Vortex-assisted dispersive micro-solid phase extraction based on nanostructured imprinted polymer: A comparison study between spectrophotometric and solution scanometric techniques.

In the present work, methyl red molecularly imprinted polymeric (MR-MIP) nanostructure was synthesized using the precipitation polymerizations for the separation of MR dye from aqueous media. The as-prepared MIP was characterized using colorimetry, infrared (IR) spectroscopy, and scanning electron microscopy (SEM). In addition, vortex-assisted dispersive micro-solid phase extraction (VAD-µSPE) based on MIP nanostructure was accomplished as a simple and efficient method for selective preconcentration of low amounts of MR from aqueous solutions.

The role of cell surface proteins gene expression in diagnosis, prognosis, and drug resistance of colorectal cancer: In silico analysis and validation.

Cell surface proteins (CSPs) are an important type of protein in different essential cell functions. This study aimed to distinguish overexpressed CSPs in colorectal cancer to investigate their biomarker, prognosis, and drug resistance potential. Raw data of three datasets including 1187 samples was downloaded then normalization and differential expression were performed.

Magnetic dual-template molecularly imprinted polymer based on syringe-to-syringe magnetic solid-phase microextraction for selective enrichment of p-Coumaric acid and ferulic acid from pomegranate, grape, and orange samples.

Magnetic dual-template molecularly imprinted polymer (FeO@SiO-MDMIP) was prepared to enrich and determine both p-Coumaric acid (p-CA) and ferulic acid (FA) based on syringe-to-syringe magnetic solid-phase microextraction (SS-MSPME). The obtained MDMIP was characterized and recognized, and then its adsorbing performance was studied. Based on the results, the FeO@SiO-MDMIP indicated selective recognition towards p-CA and FA with large adsorption capacity.

Supramolecular-Based Ultrasonic-Assisted Dispersion Solidification Liquid-Liquid Microextraction of Copper and Cobalt Prior to Their Flame Atomic Absorption Spectrometry Determination.

In the present work, a simple, efficient, and environmentally friendly method was proposed for the simultaneous preconcentration and determination of minute quantities of copper and cobalt in water samples and serum by a supramolecular-based ultrasonic-assisted dispersion solidification liquid-liquid microextraction (DSLLME) procedure coupled with flame atomic absorption spectrometry. The procedure is based on the DSLLME of copper and cobalt with decanoic acid-tetrahydrofuran supramolecular solvent (SUPRAS) in the presence of sodium diethyldithiocarbamate, as the complexing agent. SUPRAS is a nanostructured liquid generated from amphiphiles through a sequential self-assembly process occurring on two scales, molecular and nano.

Bioactivity-guided isolation of new antiproliferative compounds from Juniperus foetidissima Willd.

Based on a literature survey on cytotoxic medicinal plants, Juniper species were identified as interesting source of antitumor compounds. Using bioassay-guided fractionation against Caov-4 cancer cells on acetone extract of leaves and branchlets of Juniperus foetidissima led to the isolation of a new 3H-benzofuaran-2-one: 4-methyl-3-methoxy-3H-benzofuaran-2-one (1), a new sesquiterpene: 4,9(α)-dihydroxy-nardosin-6-en (2) and an already known labdane-type diterpene: 15-hydroxy-8(17),13(E)-labdadiene-19-carboxilic acid (3). Compounds 1-3 exhibited cytotoxic effects, with moderate cytotoxicity against the EJ-138 bladder and CAOV-4 ovary cancer cell lines.

Noise facilitation in associative memories of exponential capacity.

Recent advances in associative memory design through structured pattern sets and graph-based inference algorithms have allowed reliable learning and recall of an exponential number of patterns that satisfy certain subspace constraints. Although these designs correct external errors in recall, they assume neurons that compute noiselessly, in contrast to the highly variable neurons in brain regions thought to operate associatively, such as hippocampus and olfactory cortex. Here we consider associative memories with boundedly noisy internal computations and analytically characterize performance.

Nonbinary associative memory with exponential pattern retrieval capacity and iterative learning.

We consider the problem of neural association for a network of nonbinary neurons. Here, the task is to first memorize a set of patterns using a network of neurons whose states assume values from a finite number of integer levels. Later, the same network should be able to recall the previously memorized patterns from their noisy versions.

Spectrophotometeric determination of trace amounts of Al3+ ion in water samples after cloud point extraction using quinizarin as a complexing agent.

In this study, cloud point extraction was used for the preconcentration of Al(3+) ion after the complex formation with 1,4-dihydroxy-9,10-anthraquinone (Quinizarin [QUIN]), and subsequent analysis by spectrophotometeric method, using Triton X-114 as surfactant. The optimal extraction and reaction conditions were studied (i.e.

Flame atomic absorption spectrometric (FAAS) determination of copper, iron and zinc in food samples after solid-phase extraction on Schiff base-modified duolite XAD 761.

The present study involves the development of solid-phase extraction (SPE) procedure for the preconcentration of trace amounts of copper (Cu(2+)), iron (Fe(3+)) and zinc (Zn(2+)) ions on duolite XAD 761 modified by bis(2-hydroxyacetophenone)-2,2-dimethyl-1,3-propanediimine(BHAPDMPDI). The complexation between the metal ions and the proposed ligand was investigated potentiometrically. The metal ions retained on the sorbent were quantitatively determined via complexation with BHAPDMPDI.

Hydrothermal synthesis, experimental and theoretical characterization of a novel cocrystal compound in the 2:1 stoichiometric ratio containing 6-methyluracil and dipicolinic acid.

This paper reports the hydrothermal synthesis, experimental and theoretical studies of a novel cocrystal compound in the 2:1 stoichiometric ratio of 6-methyluracil (6mu) and dipicolinic acid (pydcH(2)) formulated as [6mu](2)[pydcH(2)] (1), for the first time. DFT calculations were performed to access the most possible geometry of the title cocrystal compound. All calculations were carried out with the B3LYP hybrid density functional level and 6-311+G(d,p) basis sets.

Construction of a new Cu2+ coated wire ion selective electrode based on 2-((2-(2-(2-(2-hydroxy-5-methoxybenzylidene amino)phenyl)disufanyl)phenylimino)methyl)-4-methoxyphenol Schiff base.

In this article a new coated platinum Cu(2+) ion selective electrode based on 2-((2-(2-(2-(2-hydroxy-5-methoxybenzylideneamino)phenyl)disufanyl)phenylimino) methyl)-4-methoxyphenol Schiff base (L(1)) as a new ionophore is described. This sensor has a wide linear range of concentration (1.2 × 10(-7)-1.

SDS-coated Sepabeads SP70-modified by 4-[(E)-3-phenylallylidene) amino] benzenethiol as new efficient solid phase for enrichment and determination of copper, nickel, chromium, and zinc ions in soil, plants, and mint water samples.

A sensitive and simple method based on modification of Sepabeads SP70 by 4-((E)-3-phenylallylidene) amino) benzenethiol for the simultaneous preconcentration of trace amounts of copper (Cu), nickel (Ni), chromium (Cr), and zinc (Zn) ions in some real samples has been reported. The metal ions which are retained as respective complexes on solid phase were eluted with 8 ml of 4 M nitric acid. The influences of different analytical parameters including pH, ligand amount and Sepabeads SP70 and SDS amount, eluting solutions, and sample volume on metal ions recoveries were investigated.

Preparation of a new chromium(III) selective electrode based on 1-[(2-hydroxy ethyl) amino]-4-methyl-9H-thioxanthen-9-one as a neutral carrier.

A new chromium carbon paste electrode sensor based on a carbon paste electrode containing 1-[(2-hydroxy ethyl) amino]-4-methyl-9H-thioxanthen-9-one (AMTX) as new carrier has been prepared. The influence of carbon paste ingredients including sodium tetraphenylborate (NaTPB), ionophore, Nujol and graphite powder on the electrode response has been investigated. The best performance characteristics for the electrode was obtained with a carbon:NaTPB:Nujol:AMTX in the mass ratio of (400:1.

Construction of new iodide ion-selective electrodes based on new ionophores: comparison of the effect of structures and additives on electrode response.

New iodide ion-selective electrodes based on bis(2-methyl-3-phenyl-propenylidene)1,2-propanediamine)mercury(II) bromide [Hg(BMPPMB)Br2], bis (5-bromo salicylaldehyde) ethylenediimine vanadyl(IV) [VO(5-Br Salen)], and bis(5-bromo salicylaldehyde) ethylenediimine uranyl(VI) [UO2(5-Br Salen)] carriers are described. The electrodes exhibited Nernstian slopes of -58.3 +/- 1.