Changes in importin levels promote nuclear proteasomal degradation of cell cycle-related proteins during THP-1 monocyte-to-macrophage differentiation.

Importin family nucleocytoplasmic transport receptors share thousands of cargo proteins. To elucidate cell regulatory mechanisms via transport regulation, we analyzed the levels of transport receptors by western blotting and quantified the total cellular and nuclear proteins during monocyte-to-macrophage differentiation of THP-1 cells using mass spectrometry. Importin-α1 decreased and importin-α5 increased during the differentiation.

Isolation, Amino Acid Sequences, and Plausible Functions of the Galacturonic Acid-Binding Egg Lectin of the Sea Hare Aplysia kurodai.

Egg lectins occur in a variety of animals ranging from mollusks to vertebrates. A few examples of molluscan egg lectins have been reported, including that of the sea hare ; however, their biological functions in the egg remain unclarified. We report the isolation, determination of primary structure, and possible functions of lectin (AKL) from the egg mass of .