Publications by authors named "Shohei Takada"

Tellurium (Te) is a chalcogen element like sulfur and selenium. Although it is unclear whether Te is an essential nutrient in organisms, unique Te metabolic pathways have been uncovered. We have previously reported that an unknown Te metabolite (UKTe) was observed in plants exposed to tellurate, a highly toxic Te oxyanion, by liquid chromatography-inductively coupled plasma mass spectrometer (LC-ICP-MS).

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Tellurium (Te) is an industrially useful element but causes environmental contamination. The formation of biogenic Te nanorods (Te-BgNRs) in plants is one of the Te detoxification pathways associated with the phytoremediation of Te because Te-BgNRs contain low-toxicity Te at high densities. In this study, we investigated the mechanism of Te-BgNR formation in a common unicellular green alga, Chlamydomonas reinhardtii, on the basis of elemental analysis by inductively coupled plasma mass spectrometry (ICP-MS).

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Background: Biosynthesis of Te nanoparticles may occur in higher plants exposed to Te, as reported on microorganisms. However, unambiguous observations of the biogenic nanoparticles (BgNPs) of Te in plants are lacking. Hence, in this study, we investigated the formation of insoluble BgNPs of Te in garlic (Allium sativum) as a model plant.

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Article Synopsis
  • - The study used single-cell inductively coupled plasma-mass spectrometry (SC-ICP-MS) to analyze the elemental composition of yeast, green algae, and red blood cells, detecting elements like phosphorus, sulfur, magnesium, zinc, and iron.
  • - Results from SC-ICP-MS for yeast and green algae were consistent with traditional methods involving acid digestion, while some discrepancies were noted for red blood cells.
  • - Overall, SC-ICP-MS proved effective for analyzing different cell types—fungus, plant, and animal—indicating its potential for various biological studies.
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In the course of screening for antimalarial agents, five tropolone compounds were isolated from the culture broth of Penicillium sp. FKI-4410. Two were known compounds, puberulic acid and stipitatic acid.

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Objective: The concept of "lung age" is thought to be useful for understanding pulmonary function. In this study, we validated "lung age" to detect pulmonary function abnormalities in pulmonary diseases.

Methods: We used both spirometry and an electronic FEV(1)/FEV(6) meter (FEV(6) meter) to perform pulmonary function tests.

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