Publications by authors named "Shohei Ochi"
Sex bias is known in the prevalence/pathology of neurodevelopmental disorders. Sex-dependent differences of the certain brain areas are known to emerge perinatally through the exposure to sex hormones, while gene expression patterns in the rodent embryonic brain does not seem to be completely the same between male and female. To investigate potential sex differences in gene expression and cortical organization during the embryonic period in mice, we conducted a comprehensive analysis of gene expression for the telencephalon at embryonic day (E) 11.
View Article and Find Full Text PDFPax6 is a sequence-specific DNA binding transcription factor that positively and negatively regulates transcription and is expressed in multiple cell types in the developing and adult central nervous system (CNS). As indicated by the morphological and functional abnormalities in spontaneous mutant rodents, Pax6 plays pivotal roles in various biological processes in the CNS. At the initial stage of CNS development, Pax6 is responsible for brain patterning along the anteroposterior and dorsoventral axes of the telencephalon.
View Article and Find Full Text PDFThe significance of gene expression dynamics in neural stem cell regulation.
Proc Jpn Acad Ser B Phys Biol Sci
July 2021
Neural stem cells (NSCs) actively proliferate and generate neurons and glial cells (active state) in the embryonic brain, whereas they are mostly dormant (quiescent state) in the adult brain. The expression dynamics of Hes1 are different between active and quiescent NSCs. In active NSCs, Hes1 expression oscillates and periodically represses the expression of proneural genes such as Ascl1, thereby driving their oscillations.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the role of Hes1 oscillations in neural development by creating two types of knock-in mice with altered Hes1 gene lengths.
- Type-1 mice, which had shortened Hes1 oscillations, exhibited significantly smaller brains, reduced neural progenitor cell (NPC) proliferation, and increased cell death compared to wild-type mice.
- In contrast, type-2 mutant mice appeared normal, suggesting that maintaining robust Hes1 oscillations is crucial for proper brain development and neurogenesis timing.
Article Synopsis
- - Hair cells are crucial for hearing and balance but are hard to regenerate in mammals, presenting challenges in treating inner ear disorders due to limited access to human inner ear tissues.
- - Researchers successfully generated hair cell-like cells from mouse induced pluripotent stem (iPS) cells by following a specific stepwise protocol involving differentiation and co-culturing with mouse utricle tissues.
- - Findings suggest that certain factors from mouse utricle stromal tissues may be key to inducing hair cells from iPS cells, which is promising for regenerative therapies.