Long-term survival in non-human primates of stem cell-derived, MHC-unmatched corneal epithelial cell sheets.

When corneal epithelial stem cells residing in the corneal limbus become dysfunctional, called a limbal stem cell deficiency (LSCD), corneal transparency is decreased, causing severe vision loss. Transplantation of corneal epithelial cell sheets (CEPS) derived from stem cells, including induced pluripotent stem cells, is a promising treatment for LSCD. However, the potential effect of human leukocyte antigen (HLA) concordance on CEPS transplantation has not been addressed.

Generation of knockout rabbits with X-linked severe combined immunodeficiency (X-SCID) using CRISPR/Cas9.

Severe immunodeficient mice are widely used to examine human and animal cells behaviour in vivo. However, mice are short-lived and small in size; while large animals require specific large-scale equipment. Rabbits are also commonly employed as experimental models and are larger than mice or rats, easy to handle, and suitable for long-term observational and pre-clinical studies.

Structures of Magnesium Oxide Cluster Cations Studied Using Ion Mobility Mass Spectrometry.

Structural assignments of gas-phase magnesium oxide cluster cations, MgO ( ≤ 24), have been achieved from a comparison of experimental collision cross sections (CCSs) measured using ion mobility mass spectrometry and theoretical CCSs calculated for equilibrium structures optimized by quantum chemical calculations. Various structures based on rock-salt and hexagonal-tube structures were assigned for = 5-13 and 15. On the other hand, only rock-salt type structures were assigned for = 4, 14, 16-21, and 24.