[Glutamine Regulates the Antitumor Activity of CD8 T Cells].

The cancer immunotherapies based on adoptive T cell therapy(ACT)has been receiving increased attention by improvement of the curative effect. T cells for ACT are harvested from the patient, then activated and expanded in vitro. However, in vitro activated T cells frequently show dysfunction after adoptive transfer, such as the exhaustion and the senescence.

Histone H3K27 Demethylase Negatively Controls the Memory Formation of Antigen-Stimulated CD8 T Cells.

Although the methylation status of histone H3K27 plays a critical role in CD4 T cell differentiation and its function, the role of Utx histone H3K27 demethylase in the CD8 T cell-dependent immune response remains unclear. We therefore generated T cell-specific Cd4-Cre Tg ( KO) mice to determine the role of Utx in CD8 T cells. Wild-type (WT) and KO mice were infected with expressing OVA to analyze the immune response of Ag-specific CD8 T cells.

Reinforce the antitumor activity of CD8 T cells via glutamine restriction.

The antitumor activity of activated CD8+ T cells in the tumor microenvironment seems to be limited due to their being metabolically unfit. This metabolic unfitness is closely associated with T-cell exhaustion and impairment of memory formation, which are barriers to successful antitumor adoptive immunotherapy. We therefore assessed the role of glutamine metabolism in the antitumor activity of CD8 T cells using a tumor-inoculated mouse model.

Menin Plays a Critical Role in the Regulation of the Antigen-Specific CD8+ T Cell Response upon Listeria Infection.

Menin, a tumor suppressor protein, is encoded by the MEN1 gene in humans. Certain germinal mutations of MEN1 induce an autosomal-dominant syndrome that is characterized by concurrent parathyroid adenomas and several other tumor types. Although menin is also expressed in hematopoietic lineages, its role in CD8 T cells remains unclear.