[Study of the effect of Unifuzol on cognitive impairment and damage to the hippocampus and cerebral cortex during course administration to rats with bilateral stenosis of the common carotid arteries, causing chronic circulatory failure].

Objective: To study the effect of Unifuzol (L-arginine sodium succinate) on cognitive impairment, cerebral blood flow, and damage to the tissues of the hippocampus and cerebral cortex during a 10-day course of administration to rats with chronic cerebral ischemia (CCI) caused by bilateral stenosis of the common carotid arteries (CCA).

Material And Methods: The study was conducted on male rats with CCI caused by bilateral stenosis of the CCA by 60%. 40 days after surgery, rats received Unifusol (21, 42 and 84 ml/kg), nicergoline (10 mg/kg), citicoline (500 mg/kg) or placebo (0.

Neuroprotective action of Cortexin, Cerebrolysin and Actovegin in acute or chronic brain ischemia in rats.

This study was the first to compare the neuroprotective activity of Cerebrolysin®, Actovegin® and Cortexin® in rodent models of acute and chronic brain ischemia. The neuroprotective action was evaluated in animals with acute (middle cerebral artery occlusion) or chronic (common carotid artery stenosis) brain ischemia models in male rats. Cortexin® (1 or 3 mg/kg/day), Cerebrolysin® (538 or 1614 mg/kg/day) and Actovegin® (200 mg/kg/day) were administered for 10 days.

Expression of Beclin-1 and LAMP-2 in Rat Hippocampus under Conditions of Simulated Gravity Overload in the Caudocranial Vector.

Structural changes in the rat hippocampus in response to chronic cerebrovascular disorders induced by gravity exposure in the caudocranial vector were studied. Qualitative and quantitative morphological analysis detected significant cytoarchitectonic changes in the pyramidal layer: spongiosis, manifest pericellular and perivascular edema, and a drastic increase in the counts of pyramidal neurons with signs of impairment in all hippocampal zones. The density of perikarya in the pyramidal layer decreased.

Effect of Combined Stress on Morphological Changes and Expression of NO Synthases in Rat Ventral Hippocampus.

Adult rats were subjected to 7-day combined stress with stochastic changes of stressors of different modalities (noise, vibration, pulsating bright light) along with mobility restriction and elevated temperature in the chamber during stress exposures (daily 30-min sessions). Circulatory disorders, inhibition of endothelial NO-synthase expression in endothelial cells of the microcirculatory bed, perivascular edema, pronounced degenerative changes, and enhanced expression of inducible NO synthase in CA3 pyramidal neurons in the ventral hippocampus of stressed 12-month-old rats were observed. These findings can attest to the involvement NOdependent mechanisms and different contribution of NO synthase isoforms into the formation of hippocampal neuronal damage.

Patterns of TRPM7 expression in hypothalamic and hippocampal neurons in modeling of nutritional magnesium deficiency.

Patterns of expression of TRPM7, the major cellular magnesium transporters in neurons of the hypothalamic region and hippocampus, were studied immunohistochemically. Multidirectional nature and different levels of the expression of the above antigen were revealed during modeled magnesium deficiency with regard to structural and functional features of neuron organization in the hypothalamic paraventricular and supraoptic nuclei as well as hippocampal field CA1 and CA3. Changes in the structural characteristics of neurons in the studied areas (absolute and relative indicators) and TRPM7 expression patterns were quantitatively analyzed considering the data on the role of the studied antigen in magnesium homeostasis, cell damage, and compensation.

[Squamosus cancer of head and neck area. Problems of the surgical treatment of the regional metastasis].

Unlabelled: In the structure of oncology disease head and neck tumors consist of about 20%. For the cancer of the larynx, pharyngolaryngeal cancer, cancer of the mouth and oropharyngeal cancer next factors have influence for the treatment planning: spreding of the tumor, presence of the regional and distant metastasis and general state of the patient.

Aim Of The Work: surgery approaches for the treatment of the regional metastasis for above mentioned tumors are determined.

Simple and efficient microwave assisted N-alkylation of isatin.

We present herein the results of microwave promoted N-alkylations of isatin (1)with different alkyl, benzyl and functionalized alkyl halides. Reactions were carried out under different conditions, always employing methodologies compatible with MW assisted chemistry. Generation of isatin anion employing diverse bases and solvents or using the preformed isatin sodium salt was tested.

[The morphofunctional state of the blood-brain barrier at the level of the medulla oblongata in experimental West Nile encephalitis].

Pronounced clinical and morphological changes were found in the neurons and microcirculatory bed (MCB) vessels at the level of the medulla oblongata in experimental West Nile encephalitis (WNE). At the same time, the magnitude of changes in MCB vessels and nerve cells in the cores of the medulla oblongata correlated with the state of the surrounding astrocytic glia. It is suggested that the intensive astroglial reaction revealed during this experimental WNE reproduction promotes the prevention of neuronal lesion.

[Astrocytic morphological responses in different murine brain regions in the simulation of West Nile fever].

The brains from the mice infected intraperitoneally (with development of these or those symptoms of viral encephalitis) or intramuscularly with the Astrakhan West Nile fever virus strain (AcT 986) was pathomorphologically studied. Clinicomorphological changes in the neurons and vessels of the microcirculatory bed were found in the dorsal regions of medulla oblongata. Immunohistochemicall analysis showed that mature and/or activated astrocytes increased in number, largely in the animals without clinical manifestations of encephalitis.

[The role of bacterial growth autoregulators (alkyl hydroxybenzenes) in the response of staphylococci to stresses].

The investigation of the response of a batch culture of Staphylococcus aureus to exogenous alkyl-substituted hydroxybenzenes (AHBs), chemical analogues of anabiosis autoinducers, showed that C1-AHB at concentrations from 5 microM to 1.5 mM did not influence the culture growth, whereas the more hydrophobic C6-AHB inhibited it at concentrations of 0.5 mM and higher.

[ICO-35 monoclonal antibodies to the antigen of acute lymphoblastic leukemia].

ICO-35 monoclonal antibodies (Mabs) were produced following BALB/c mouse immunization with peripheral blood cells from a patient with lymphoid type of chronic myeloid leukemia in blast crisis (SML BC) ICO-35. Mabs detect antigen on CD10-positive cells from patients with acute lymphoblastic leukemia, CML BC, CD10-positive cells of Reh line and are not bound to other cells. Comparative studies of reactivity of ICO-35 and K 503 Mabs to CD10 antigen revealed their similarity.