Association between type 1 diabetes mellitus and remitting seronegative symmetrical synovitis with pitting edema: a case report.

A 59-year-old female with type 1 diabetes and RS3PE had HLA types known to be associated with both diseases. Type 1 diabetes patients suffering from polyarthritis and pitting edema should be examined for possible RS3PE and glucocorticoid therapy may be indicated despite the diabetes.

Fertilization Potential of the Medaka Egg: (fertilization potential/depolarization/hyperpolarization/Ca /medaka).

Fertilization of the medaka egg in 10% Ringer's solution generates a depolarization of 4 mV just before the appearance of a characteristically longer hyperpolarization (25). The depolarization appears to be the result of a nonspecific leak triggered by sperm stimulation and the amplitude of the depolarization is thought to be independent of [Ca ] (25). We have investigated the ionic dependence of this depolarization.

Effects of Various Antibiotics on the Excitability of Newt Ectodermal Cells in vitro: (amphibian embryos/epidermal action potential/cell culture/antibiotics).

With the addition of actinomycin D (AMD), cycloheximide (CH) or tunicamycin (TM) to a culture medium at 23°c, the epidermal action potentials (EAPs) of the cultured epidermal explants or monolayered cell colonies taken from newt embryos were examined for confirmation of their relationship to transcriptional and translational events. EAPs of ectodermal cells from the pregastrula were first recorded after 84 hr of monolayered cell culture in FCS-Steinberg's medium. The addition of 10 μg/ml CH or 1 μg/ml TM for 36 hr prevented the appearance of these potentials in cells precultured in FCS-Steinberg's medium for 60 hr.

Genesis of Epidermal Action Potentials in Cytokinesis Arrested Xenopus Embryos: (amphibian embryo/epidermal action potential/cytokinesis/cytochalasin B/cell differentiation).

When Xenopus embryos were treated continuously with cytochalasin B (3-10 μg/ml) from the 8 cell stage, cleavage arrested embryos in various degrees were observed. In 3-5 μg/ml cytochalasin B, cytokinesis was inhibited at the midblastula stage and pigment granules remained at the cell cortex of the animal pole. These cells showed epidermal like action potentials when the control embryos (St.

The Genesis and Transmission of Epidermal Potentials of Newt Embryonic Cells in Vivo and in Vitro: (newt embryo/cell culture/epidermal differentiation/epidermal action potential/transmission).

The genesis and transmission of action potentials in epidermal cells of a newt (Cynops pyrrhogaster) embryo were investigated quantitatively in vivo during development and in vitro in the absence of nerve cells. Typical action potentials, composed of a fast spike followed by a slow action potential, can be recorded from any of the epidermal cells from Stage 24/25 to 35/36. The potential is graded with current intensity, and only the slow component induces transmission to other epidermal cells.

Mitosis in Beating Cardiac Muscle Cells from Newt Embryos In Vitro.

Cardiac muscle cells from newt embryos were cultured at relatively low cell density. Within 10 days in culture, 2 cell types (spindle and flat type) were distinguished both among beating and non-beating cells. Mitosis in single beating cells was frequently observed both in spindle and flat cells.

Electrical Properties of Toad Oocytes During Maturation and Activation.

The full-grown oocytes of the toad Bufo bufo japonicus, whether in follicular layer or not, had a membrane potential of about -50 mV in De Boer's solution (DB), but underwent a deep hyper-polarization of up to -90 mV when pretreated with Ca, Mg-free EDTA-solution. Regardless of the magnitude of their resting potentials, the defolliculated oocytes exposed to progesterone underwent a gradual depolarization before the germinal vesicle breakdown and retained membrane potential at a level of -10 mV until 18 hr post hormone treatment (PHT), the stage of the second meiotic metaphase. A positive-going activation potential of a magnitude of 70 mV was recorded in the oocytes when pricked at 18 hr PHT as well as in uterine eggs 3-5 min after insemination.

INTER AND INTRATISSUE COMMUNICATION DURING AMPHIBIAN DEVELOPMENT.

The changes of electrical communication between various tissues of the newt (Cynops pyrrhogaster) embryo during development have been investigated by measuring electrotonic potentials at various interelectrode distances. In general, cells of the same tissue are electrically coupled from gastrulation up to closure of the neural tube. Notochordal cells, however, are an exception in that cell coupling decreases during stages 22-23 in comparison to earlier stages.

EFFECTS OF MEDIA OF DIFFERENT IONIC COMPOSITION ON THE ACTIVATION POTENTIAL OF ANURAN EGG CELLS.

Activation potentials of the eggs of two anuran species, Bufo vulgaris formosus and Rana rugosa, were measured in media of different ionic composition, and the mechanism of their generation is discussed. The same trends of ionic effects upon the activation potential were consistently obtained in both species. The membrane potential of the unactivated eggs was negative with respect to tap water, Ringer's solution, and the media described below except isotonic KCl and KNO , in which the potentials were nearly zero or sometimes slightly positive.