Construction of a Convenient and Highly Sensitive Sensor for the Detection of Myo in Serum Based on ELI-SERS.

Acute myocardial infarction (AMI) is one of the most common causes of sudden death in cardiovascular disease, and myoglobin (Myo) is the first protein to be released in the blood after the attack, which is an important biomarker for clinical detection of AMI. The "Golden Rescue Time" for acute myocardial infarction is to intervene within the first 30 min after the attack; therefore, a rapid and accurate Myo detection method is needed urgently. In this study, we designed a combined enzyme-linked immunosorbent assay (ELISA) technique with surface-enhanced Raman scattering (SERS) immunoassay (ELI-SERS), which integrates the small sample volume, ease of operation, and excellent linearity of ELISA while utilizing the SERS technique and selecting the molecule with the Raman signal (IR-808), which is in resonance with the excitation wavelength, for further signal enhancement.

Monodisperse, Highly Spherical, Single Crystalline Au Nanospheres for Uniform and Reproducible Hot Spots in Surface-Enhanced Raman Scattering at the Single-Particle Level.

Hot spots can generate intense local electromagnetic (EM) fields, thereby boosting diverse innovative applications. However, these applications may face challenges due to their subtle structural changes that can significantly impact their EM field strength. Herein, we report a large-scale synthesis of monodisperse, highly spherical, single crystalline (SC) Au nanospheres (Au NSs) with tunable sizes ranging from 38 to 92 nm for constructing uniform and reproducible hot spots with a nanosphere-on-mirror (NSoM) configuration.

A broad neutralizing nanobody against SARS-CoV-2 engineered from an approved drug.

SARS-CoV-2 infection is initiated by Spike glycoprotein binding to the human angiotensin-converting enzyme 2 (ACE2) receptor via its receptor binding domain. Blocking this interaction has been proven to be an effective approach to inhibit virus infection. Here we report the discovery of a neutralizing nanobody named VHH60, which was directly produced from an engineering nanobody library based on a commercialized nanobody within a very short period.

First report of causing leaf spot on in central China.

, commonly known as watershield and referred to as 'Chun Cai' in Chinese, is a worldwide aquatic vegetable. It has long been regarded as health- promoting vegetable due to production of mucilage in young shoots, and thus has gained popularity in China. In September 2022, a leaf spot disease was observed at the National Aquatic Vegetable Germplasm Resource Nursery located in Wuhan, Hubei province, China.

Bioinspired Tumor Calcification-Guided Early Diagnosis and Eradication of Hepatocellular Carcinoma.

Tumor calcification is found to be associated with the benign prognostic, and which shows considerable promise as a somewhat predictive index of the tumor response clinically. However, calcification is still a missing area in clinical cancer treatment. A specific strategy is proposed for inducing tumor calcification through the synergy of calcium peroxide (CaO)-based microspheres and transcatheter arterial embolization for the treatment of hepatocellular carcinoma (HCC).

The Gβ-like protein Bcgbl1 regulates development and pathogenicity of the gray mold Botrytis cinerea via modulating two MAP kinase signaling pathways.

The fungal Gβ-like protein has been reported to be involved in a variety of biological processes, such as mycelial growth, differentiation, conidiation, stress responses and infection. However, molecular mechanisms of the Gβ-like protein in regulating fungal development and pathogenicity are largely unknown. Here, we show that the Gβ-like protein gene Bcgbl1 in the gray mold fungus Botrytis cinerea plays a pivotal role in development and pathogenicity by regulating the mitogen-activated protein (MAP) kinases signaling pathways.

Intelligent convolution neural network-assisted SERS to realize highly accurate identification of six pathogenic .

Based on label-free SERS technology, the relationship between the Raman signals of pathogenic microorganisms and purine metabolites was analyzed in detail. A deep learning CNN model was successfully developed, achieving a high accuracy rate of 99.7% in the identification of six typical pathogenic species within 15 minutes, providing a new method for pathogen identification.

Connecting Calcium-Based Nanomaterials and Cancer: From Diagnosis to Therapy.

As the indispensable second cellular messenger, calcium signaling is involved in the regulation of almost all physiological processes by activating specific target proteins. The importance of calcium ions (Ca) makes its "Janus nature" strictly regulated by its concentration. Abnormal regulation of calcium signals may cause some diseases; however, artificial regulation of calcium homeostasis in local lesions may also play a therapeutic role.

Application of Self-Assembly Nanoparticles Based on DVDMS for Fenton-Like Ion Delivery and Enhanced Sonodynamic Therapy.

Upon harnessing low-intensity ultrasound to activate sonosensitizers, sonodynamic therapy (SDT) induces cancer cell death through the reactive oxygen species (ROS) mediated pathway. Compared with photodynamic therapy (PDT), SDT possesses numerous advantages, including deeper tissue penetration, higher accuracy, fewer side effects, and better patient compliance. Sinoporphyrin sodium (DVDMS), a sonosensitizer approved by the FDA, has drawn abundant attention in clinical research, but there are some deficiencies.

Activatable Second Near-Infrared Fluorescent Probes: A New Accurate Diagnosis Strategy for Diseases.

Recently, second near-infrared (NIR-II) fluorescent imaging has been widely applied in biomedical diagnosis, due to its high spatiotemporal resolution and deep tissue penetration. In contrast to the "always on" NIR-II fluorescent probes, the activatable NIR-II fluorescent probes have specific targeting to biological tissues, showing a higher imaging signal-to-background ratio and a lower detection limit. Therefore, it is of great significance to utilize disease-associated endogenous stimuli (such as pH values, enzyme existence, hypoxia condition and so on) to activate the NIR-II probes and achieve switchable fluorescent signals for specific deep bioimaging.

Surface engineering of oncolytic adenovirus for a combination of immune checkpoint blockade and virotherapy.

Advances in the development of modern cancer immunotherapy and immune checkpoint inhibitors have dramatically changed the landscape of cancer treatment. However, most cancer patients are refractory to immune checkpoint inhibitors because of low lymphocytic tumor infiltration and PD-L1 expression. Evidence suggests that viral oncolysis and immune checkpoint inhibitors have a synergistic effect that can improve the response to immune checkpoint inhibitors.

Multifunctional supramolecular self-assembly system for colorimetric detection of Hg, Fe, Cu and continuous sensing of volatile acids and organic amine gases.

A novel multifunctional gelator (1) based on an azobenzene derivative was designed and characterized. This compound could gelate some solvents including hexane, petroleum ether, DMSO, acetonitrile and ethanol through a heating-cooling procedure. The self-assembly process in different solvents was studied by means of UV-vis absorption and Fourier transform infrared (FTIR) spectra, field emission scanning electron microscopy (FESEM), rheological measurements, X-ray powder diffraction and water contact angle experiments.

Chloroplast Translation Initiation Factors Regulate Leaf Variegation and Development.

Chloroplast development requires the coordinated expressions of nuclear and chloroplast genomes, and both anterograde and retrograde signals exist and work together to facilitate this coordination. We have utilized the Arabidopsis yellow variegated (var2) mutant as a tool to dissect the genetic regulatory network of chloroplast development. Here, we report the isolation of a new (to our knowledge) var2 genetic suppressor locus, SUPPRESSOR OF VARIEGATION9 (SVR9).

Early diet intervention to reduce the incidence of hepatic encephalopathy in cirrhosis patients: post-Transjugular Intrahepatic Portosystemic Shunt (TIPS) findings.

Background And Objectives: Hepatic encephalopathy is a common complication in patients who have received transjugular intrahepatic portosystemic shunt (TIPS) as treatment for cirrhosis. The objective of this study was to reduce the incidence of post-TIPS hepatic encephalopathy for these patients via positive diet intervention.

Methods And Study Design: As a control group, 99 cirrhosis patients who underwent TIPS treatment in our department between August 2011 and February 2013 were chosen.

The effects of high thoracic epidural anesthesia on sympathetic activity and apoptosis in experimentally induced congestive heart failure.

Objective: To evaluate the effect of high thoracic epidural analgesia (HTEA) in congestive heart failure (CHF).

Design: Rat model of CHF.

Setting: Harbin Medical University, Harbin, Heilongjiang, China.

[Influence of central obesity on clustering of other risk variables on metabolic syndrome among adults with normal body mass index].

Objective: To study the influence of central obesity on clustering of other metabolic syndrome (MS) risk variables among adults with normal body mass index.

Methods: Through cluster multistage and random sampling methods, local people aged>35 years old with normal body mass index (BMI=18.5-24.

[A 50-year study on the epidemiology of hypertension in Heilongjiang Province of China].

Objective: To assess the epidemiological characteristics of hypertension and its attributing factors in Heilongjiang province and establish a comprehensive basis for the prevention and control of hypertension in this region.

Methods: Using the stratified chunk method, a survey lasting 50 years was conducted on a sample of 299 677 (including 158 782 males and 140 895 females) patients, aged 15 and above, residing in both rural and urban areas of Heilongjiang province from 1958 to 2007. Both blood pressure measurement and analysis on attributing factors of hypertension were carried out in 1958, 1979, 1991, 1999 and 2007.

[A cross-sectional study on impaired fasting glycaemia and diabetes mellitus in residents from Nangang district, Harbin city].

Objective: To investigate the detection rate of impaired fasting glycaemia (IFG), the prevalence of diabetes mellitus (DM) and related risk factors, the current situation on awareness, treatment and the rate of control on diabetes mellitus in residents living in Nangang district of Harbin city, Heilongjiang province.

Methods: A cross-sectional cluster sampling was carried out in residents aged over 35 years, living in Fendou community of Harbin city.

Results: Data from 3017 out of 3183 residents were analyzed.

[Prevalence and risk factors of prehypertensive status in people from the rural area of Lanxi county, Heilongjiang province].

Objective: To access the prevalence of prehypertensive stage and its associated risk factors in rural inhabitants from Lanxi county in Heilongjiang province.

Methods: Through cluster multistage and random sampling methods, local people aged > or = 15 years old in Lanxi county were selected. A survey on blood pressure and associated risk factors was carried out.

[Intensive atorvastatin therapy in patients with acute myocardial infarction].

Objective: To assess the safety and efficacy of 40 mg daily atorvastatin in patients with acute myocardial infarction.

Methods: A total of 1102 patients with AMI admitted to our hospital from 2003 to 2007 were assigned to atorvastatin 40 mg daily within 24 hours of hospitalization and continued till 3 months post discharge. Patients with LDL-C < 2.

[Prevalence, awareness, treatment, and risk factors of hypertension in Harbin, Heilongjiang Province].

Objective: To investigate the prevalence, rates of awareness, treatment, and control, and risk factors of hypertension among the residents in Harbin, Heilongjiang Province.

Methods: 5523 residents, 2325 males and 3198 females, aged 15 and over, underwent questionnaire survey to understand their general conditions, history of present disease, awareness of disease, treatment, working condition, etc, and measurement of blood pressure (BP), height, body mass, and serum glucose and triglyceride.

Results: The no-standardized prevalence of hypertension was 31.

[A cross-sectional study of impaired fasting glycaemia and diabetes mellitus in rural residents of Lanxi, Heilongjiang].

Objective: To investigate the current status of impaired fasting glycaemia (IFG), diabetes mellitus (DM) and correlated factors, as well as on the awareness, treatment and control rate of diabetes mellitus in rural residents of Lanxi, Heilongjiang.

Methods: Cross-sectional and cluster sampling method was carried out on 3480 residents over 35 years of age, in rural residents of Lanxi Pingshan, Heilongjiang province.

Results: To male, female and all, the detecting rate of IFG were 5.