The novel methyltransferase SETD4 regulates TLR agonist-induced expression of cytokines through methylation of lysine 4 at histone 3 in macrophages.

The production of inflammatory cytokines is closely related to pathogen-associated molecular pattern (PAMP)-triggered activation of the Toll-like receptor (TLR), intracellular signal transduction pathways such as MAPK and NF-κB, and histone modifications. Histone methylation, a type of histone modifications, is mainly accomplished by a class of SET family proteins containing highly conserved SET domains. In the present study, we found that SET domain-containing protein 4 (SETD4) regulated inflammatory cytokines in response to TLR agonists.

Differences in Lipopolysaccharides-Induced Inflammatory Response Between Mouse Embryonic Fibroblasts and Bone Marrow-Derived Macrophages.

Mouse embryonic fibroblasts (MEFs) are commonly used in research on the molecular mechanism(s) of inflammation because of its good response to inflammatory stimuli. However, the difference in inflammatory reaction between MEFs and macrophages, a classical inflammatory cell type, has not been identified. In this study, we report that both mRNA and protein levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in MEFs upon lipopolysaccharides (LPS) stimulation were significantly lower than those in bone marrow-derived macrophages (BMDMs).