Sterols possess immunomodulatory property: Activity guided isolation and screening of phytoconstituents of and .

Objective: To isolate the phytoconstituents from the methanolic extracts of the stem bark of and , characterize spectroscopically and screen for immunomodulatory activity on human neurophils.

Methods: A flavonoid (PGF) and an alkaloidal compound (PGA) from the extract of and a steroidal compound (FGS) and tannin fraction (FGT) from the extract of were isolated using column chromatography technique and were subjected for the spectroscopic (FT-IR, HNMR and LC-MS) and TLC studies to identify the compounds. The isolated compounds were screened for immunomodulatory activity on human neutrophils using nitroblue tetrazolium (NBT) dye test, phagocytosis of and neutrophil locomotion and chemotaxis assay at the concentration range of 100, 50, 25, 12.