Improving activity and stabilization of urease by crosslinking to nanoaggregate forms for herbicide degradation.

Bioremediation is the most effective green protocol for degradation of environmental contaminants. Present study involves carrier free urease immobilization with synthesis of its new crosslinked aggregates using two different crosslinkers, divinyl benzene (DVB) and tripropyleneglycol dimethacrylate (TPGDA) via free radical mechanism. Resulting crosslinked ureases were further converted to nanoform (CLUNAs) using solvent evaporation technique.

New Nanoaggregates of Crosslinked Laccase for Reactive Red Bioremediation.

Environmental concerns have led to an increased interest in developing green technologies for bioremediation of wastewater pollutants. In view of this, laccases have emerged as attractive green catalysts due to their applicability in oxidizing broad range of substrates. In the present work laccase was extracted from (MTCC 138) and stabilized by formation of new crosslinked laccase nanoaggregates (CLNAs) using two different crosslinkers-N,N-methylenebisacrylamide (-MBAAm) and ethyleneglycol dimethacrylate (EGDMA).

Enhanced catalytic activity of new acryloyl crosslinked cellulose dialdehyde-nitrilase Schiff base and its reduced form for nitrile hydrolysis.

Immobilization of enzymes to improve their catalytic properties is an attractive protocol which makes them suitable candidates to meet various industrial demands. Present study describes the synthesis of new acryloyl crosslinked cellulose dialdehyde (ACCD) for nitrilase immobilization. Nitrilase was immobilized onto ACCD via Schiff base formation i.

New glucose oxidase-immobilized stimuli-responsive dextran nanoparticles for insulin delivery.

Designing strategies for the use of biopolymer-based nanoparticles as drug delivery carriers is a considerable challenge in pharmaceutical science. Present study reports synthesis of a novel glucose responsive and in-vitro pH triggered insulin delivery system comprised of glucose oxidase immobilized on acryloyl crosslinked dextran dialdehyde (ACDD) nanoparticles. Scanning electron microscopy, transmission electron microscopy and particle size analysis data revealed that these carriers possess nanosize which is an important parameter for drug delivery applications.