Publications by authors named "Shivaiah Nagaraju"

The primary objective of the current investigation was the biosynthesis of Phy-AgNPs by the endophytic fungus Phyllosticta owaniana (extracted from Abrus precatorius) and the evaluation of the secondary metabolites from the ethyl acetate extract of P. owaniana cultivated by submerged fermentation. Utilizing bioanalytical strategies, Phy-AgNPs were characterized.

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The assay of mammalian hyaluronidases (HAases) is important in understanding the role of the hyaluronan-hyaluronidase (HA-HAase) system in various pathophysiological processes. Despite several quantitative assay method options, the Morgan-Elson colorimetric method modified by Reissig et al is considered the best for determining the activity in clinical samples. However, the sensitivity of the method was greatly limited by presence of protein above 400 microg due to turbidity interference that led to chromogen quenching.

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Partitagin, a Zn-containing hemorrhagic metalloprotease from Hippasa partita spider venom gland extract has been purified and studied for its dermonecrotic and myonecrotic effects. In the present study, Partitagin has been studied for its effect on fibrinogen, fibrin and platelet aggregation. Partitagin incubated with fibrinogen preferentially cleaved Bβ chain and later γ-chain upon prolonged incubation period (16 h).

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Snakebites in rural areas of tropical and subtropical regions are commonly treated with medicinal plants. In this report, we have studied the ability of Anacardium occidentale bark extract to neutralize enzymatic as well as pharmacological effects induced by Vipera russelii venom. The extract neutralized the viper venom hydrolytic enzymes such as phospholipase, protease, and hyaluronidase in a dose dependent manner.

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Spider venom is a complex mixture of protein and peptide toxins. Hyaluronidase a 'spreading factor' has not been studied extensively in spider venom. In this paper, we describe the purification and characterization of a hyaluronidase from Hippasa partita venom gland extract.

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