Lab-on-chip (LoC) application for quality sperm selection: An undelivered promise?

Quality sperm selection is essential to ensure the effectiveness of assisted reproductive techniques (ART). However, the methods employed for sperm selection in ART often yield suboptimal outcomes, contributing to lower success rates. In recent years, microfluidic devices have emerged as a promising avenue for investigating the natural swimming behavior of spermatozoa and developing innovative approaches for quality sperm selection.

Quantification of Very Low Concentrations of Leukocyte Suspensions In Vitro by High-Frequency Ultrasound.

Accurate measurement of very low cerebrospinal fluid (CSF) white blood cell (WBC) concentration is key to the diagnosis of bacterial meningitis, lethal if not promptly treated. Here we show that high frequency ultrasound (HFUS) can detect CSF WBC in vitro in concentrations relevant to meningitis diagnosis with a much finer precision than gold standard manual counting in a Fuchs-Rosenthal chamber. WBC concentrations in a mock CSF model, in the range 0-50 WBC/μL, have been tested and compared to gold standard ground truth.

Concentration measurement of yeast suspensions using high frequency ultrasound backscattering.

This work proposes the use of an ultrasound based technique to measure the concentration of yeasts in liquid suspension. This measurement was achieved by the detection and quantification of ultrasonic echoes backscattered by the cells. More specifically, the technique was applied to the detection and quantification of Saccharomyces cerevisiae.

Comment on "Attenuation and dispersion of sound in dilute suspensions of spherical particles" [J. Acoust. Soc. Am. 108(1), 126-146 (2000)].

Comment is made on an article by Temkin [J. Acoust. Soc.