Development of two recombinase-aided amplification assays combined with lateral flow dipstick (RAA-LFD) and real-time fluorescence (RF-RAA) for the detection of Frog virus 3-like ranaviruses.

Frog virus 3-like ranaviruses (FV3-like viruses), particularly FV3 (Frog virus 3), represent typical species within the genus Ranavirus, primarily infecting amphibians and reptiles, thereby posing serious threats to aquaculture and biodiversity conservation. We designed a pair of universal primers and a probe targeting the conserved region of the major capsid protein (MCP) genes of FV3-like viruses. By integrating recombinase-aided amplification (RAA) with lateral flow dipstick (LFD) technology and real-time fluorescence (RF) modification, we established RAA-LFD and RF-RAA assays.

Optimization Study of a High-Efficiency Preservative for Ammonia-Free Concentrated Natural Rubber Latex.

Ammonia is commonly used as a preservative in the production of concentrated natural rubber latex (CNRL) and latex products; however, it poses a serious risk to human health and the environment. In this study, we investigated a thioacetamide derivative (TD) as a preservative of ammonia-free CNRL and the optimization of a stabilization system comprising potassium hydroxide (KOH), lauric acid (LA), and sodium dodecyl sulfate (SDS) to enhance its preservation effect. The results revealed that an optimal amount of TD (0.

The Gas-Sensing Properties of Ag-/Au-Modified TiCT (T=O, F, OH) Monolayers for HCHO and CH Gases.

Based on density functional theory calculations, this study analyzed the gas-sensing performance of TiCT (T=O, F, OH) monolayers modified with precious metal atoms (Ag and Au) for HCHO and CH gas molecules. Firstly, stable structures of Ag- and Au-single-atom doped TiCT (T=O, F, OH) surfaces were constructed and then HCHO and CH gas molecules were set to approach the modified structures at different initial positions. The most stable adsorption structure was selected for further analysis of the adsorption energy, adsorption distance, charge transfer, charge deformation density, total density of states, and partial density of states.

Transcriptome and Neuroendocrinome Responses to Environmental Stress in the Model and Pest Insect .

The fall armyworm, , is one of the most notorious pest insects, causing damage to more than 350 plant species, and is feared worldwide as an invasive pest species since it exhibits high adaptivity against environmental stress. Here, we therefore investigated its transcriptome responses to four different types of stresses, namely cold, heat, no water and no food. We used brain samples as our interest was in the neuroendocrine responses, while previous studies used whole bodies of larvae or moths.

Bioremediation of Heavy Metal-Contaminated Solution and Aged Refuse by Microbially Induced Calcium Carbonate Precipitation: Further Insights into .

Recently, the ability of microbial-induced calcium carbonate precipitation (MICP) to remediate heavy metals has been widely explored. was selected to remediate heavy metal-contaminated solution and aged refuse, exploring the feasibility of bioremediation of heavy metals and analyzing the changes in heavy metal forms before and after bioremediation, as well as the mechanism of remediation. The results showed that achieved remediation rates of 95%, 84%, 97%, and 98% for Cd, Pb, Zn, and Cr (III) in contaminated solution, respectively.

Effect of Praziquantel on Preventing Delayed Infection of in Buffaloes and Goats.

Schistosomiasis, caused by , continues to pose a major public health threat in East Asia, with an estimated 71 million people at risk of infection. Domestic animals, especially buffaloes and goats, serve as important reservoir hosts, facilitating the transmission of the parasite to humans. While praziquantel (PZQ) is the first-line treatment for schistosomiasis due to its broad-spectrum efficacy against adult schistosomes, its prophylactic potential is less explored.

Pathological and Molecular Characterization of Grass Carp Co-Infected with Two Species.

The grass carp () is highly susceptible to infections caused by species, particularly and . However, the immunological mechanisms underlying co-infection by these pathogens remain largely uncharted. This study investigated the pathogenesis and host immune response in grass carp following concurrent infection with and .

The micro(nano)plastics perspective: exploring cancer development and therapy.

Microplastics, as an emerging environmental pollutant, have received widespread attention for their potential impact on ecosystems and human health. Microplastics are defined as plastic particles less than 5 millimeters in diameter and can be categorized as primary and secondary microplastics. Primary microplastics usually originate directly from industrial production, while secondary microplastics are formed by the degradation of larger plastic items.

Disrupting effects of neonicotinoids and their interaction with metals on thyroid hormone, an evidence of children in a rural area, South China.

Neonicotinoids exposure was found to induce thyroid dysfunction. However, there lack of direct evidence between neonicotinoids exposure and thyroid hormone (TH) disruption in population study, especially in children, which limits the understanding on their health hazard. To fill this knowledge gap, we conducted a cross-sectional study on children of a rural area in South China (n = 88), and analyzed urinary ten neonicotinoids (including metabolites), serum TH, thyroxine-binding globulin (TBG), and thyroid stimulating hormone (TSH) levels.

Development and validation of a radiomics-visual evoked potential nomogram for preoperative prediction of visual outcome after endoscopic craniopharyngioma resection.

Objective: Craniopharyngiomas are rare, benign brain tumors that are primarily treated with surgery. Although the extended endoscopic endonasal approach (EEEA) has evolved as a more reliable surgical alternative and yields better visual outcomes than traditional craniotomy, postoperative visual deterioration remains one of the most common complications, and relevant risk factors are still poorly defined. Hence, identifying risk factors and developing a predictive model for postoperative visual deterioration is indeed necessary.

Detectability of Al18F-NOTA-HER2-BCH PET for Nodal Metastases in Patients With HER2-Positive Breast Cancer.

Purpose: The aim of this study was to compare Al18F-NOTA-HER2-BCH and 18F-FDG for detecting nodal metastases in patients with HER2-positive breast cancer on PET/CT.

Patients And Methods: In this retrospective study, 62 participants with HER2-positive breast cancer underwent both Al18F-NOTA-HER2-BCH and 18F-FDG PET/CT. Participants were pathologically confirmed as HER2-positive (IHC 3+ or IHC 2+ with gene amplification on FISH).

Characterisation of isolates from the poultry production chain in Zhejiang Province, China: antimicrobial resistance, virulence factors and genotypic profiling.

1. This study investigated antimicrobial resistance, virulence factors and genotypic profiling among isolated from three sources (poultry farms, slaughterhouses and retail markets) in the poultry production chain in Zhejiang Province, China, to assess its potential risk to public health.2.

Characterization of Thirty Germplasms of Millet Pepper ( L.) in Terms of Fruit Morphology, Capsaicinoids, and Nutritional Components.

Background: Millet peppers have rich and diverse germplasm resources. It is of great significance to characterize their phenotypes and physicochemical indicators.

Methods: 30 millet germplasms were selected to measure the fruit length and width, flesh thickness, number of ventricles, fruit stalk length, and single fruit weight, and the texture characteristics of fruit such as hardness, cohesiveness, springiness, gumminess, and chewiness were determined by a texture analyzer.

A Study of Volatile Organic Compounds in Patients with Obstructive Sleep Apnea.

: Obstructive Sleep Apnea (OSA) is a prevalent sleep disorder characterized by intermittent upper airway obstruction, leading to significant health consequences. Traditional diagnostic methods, such as polysomnography, are time-consuming and resource-intensive. : This study explores the potential of proton-transfer-reaction mass spectrometry (PTR-MS) in identifying volatile organic compound (VOC) biomarkers for the non-invasive detection of OSA.

Metabolic profiling of meningioma reveals novel subgroup-specific biologic insights and outcome dependencies.

Background: Our group and others have recently identified four molecular groups of meningioma, with unique underlying biology and outcomes. The relevance of group-specific metabolite profiles (particularly among hypermetabolic tumours), has not been explored.

Methods: We performed untargeted metabolic profiling of meningiomas representing each molecular group and WHO grade.

Cell-type-specific networks during hippocampal seizures at the micro- and macroscale.

Epilepsy is a network disorder, involving neural circuits at both the micro- and macroscale. While local excitatory-inhibitory imbalances are recognized as a hallmark at the microscale, the dynamic role of distinct neuron types during seizures remain poorly understood. At the macroscale, interactions between key nodes within the epileptic network, such as the central median thalamic nucleus (CMT), are critical to the, hippocampal epileptic process.

A carnitine transporter at the blood-brain barrier modulates sleep via glial lipid metabolism in .

To regulate brain function, peripheral compounds must traverse the blood-brain barrier (BBB), an interface between the brain and the circulatory system. To determine whether specific transport mechanisms are relevant for sleep, we conducted a BBB-specific inducible RNAi knockdown (iKD) screen for genes affecting sleep in . We observed reduced sleep with knockdown of solute carrier , a carnitine transporter, as determined by isotope flux.

Comparison of Short-term Outcomes Following Minimally Invasive (Endoscopic/Robotic) vs Open Thyroidectomy for Patients With Thyroid Cancer.

Background Selection between open thyroidectomy (OT) and minimally invasive (endoscopic/robotic) thyroidectomy (MT) for patients with thyroid cancer has been a subject of considerable debate. Comprehensive analysis of the short-term outcomes of endoscopic thyroidectomy (ET), robotic thyroidectomy (RT) and OT for thyroid cancer using a large-scale dataset is important. Methods This cohort study evaluated the outcomes of patients receiving ET, RT vs OT for thyroid cancer from January 1, 2003, to December 31, 2022.

Improved Black Phosphorus Nanocomposite Hydrogel for Bone Defect Repairing: Mechanisms for Advancing Osteogenesis.

Bone defects caused by fractures and diseases often do not heal spontaneously. They require external agents for repair and regeneration. Bone tissue engineering is emerging as a promising alternative to traditional therapies like autografts and allografts.

Quantitative immunohistochemistry analysis of breast Ki67 based on artificial intelligence.

Breast cancer is a common malignant tumor of women. Ki67 is an important biomarker of cell proliferation. With the quantitative analysis, it is an important indicator of malignancy for breast cancer diagnosis.

Treatment of Femoral Neck Fractures in Adults With Improved Biplane Double-Supported Screw Fixation and Femoral Neck System: Complications and Surgical Techniques.

Objectives: Treating femoral neck fractures remains a significant challenge for orthopedic surgeons and imposes a substantial economic burden on developing regions. Current novel internal fixation methods demonstrate excellent biomechanical performance. However, these new internal fixation methods are still associated with various complications.

The left amygdala is genetically sexually-dimorphic: multi-omics analysis of structural MRI volumes.

Brain anatomy plays a key role in complex behaviors and mental disorders that are sexually divergent. While our understanding of the sex differences in the brain anatomy remains relatively limited, particularly of the underlying genetic and molecular mechanisms that contribute to these differences. We performed the largest study of sex differences in brain volumes (N = 33,208) by examining sex differences both in the raw brain volumes and after controlling the whole brain volumes.

Total Saponins from Panax japonicus Reduced Lipid Deposition and Inflammation in Hepatocyte via PHD2 and Hepatic Macrophage-derived Exosomal miR-463-5p.

Ethnopharmacological Relevance: Panax japonicus (T. Nees) C.A.

Effect of different scanning threshold triggers on the image quality of brain computed tomography angiography: a randomized controlled trial.

Background: The scanning trigger threshold affects image quality. The aim of this study was to investigate the effect of different scanning trigger thresholds on brain computed tomography angiography (CTA) image quality.

Methods: In this prospective study, 80 patients undergoing brain CTA examinations with dual-layer CT (DLCT) were randomly divided into group A and group B, with 40 patients in each group.

Effects of patent foramen ovale in migraine: a metabolomics-based study.

Patent foramen ovale (PFO), a cardiac anatomical anomaly inducing abnormal haemodynamics, leads to a paradoxical bypass of the pulmonary circulation. PFO closure might alleviate migraines; however, clinical evidence and basic experiments for the relationship are lacking. To explore the effect of PFO on migraine, 371 migraineurs finishing blood tests and contrast transthoracic echocardiography for the detection of PFO were prospectively included.