Generic Workflow of a Highly Effective and Easy Anther Culture Method for Both Japonica and Indica Rice.

As one of the most important staple crops in the world, rice plays a pivotal role in world food security. The creation of doubled haploids based on anther culture is an important technology for rice breeding. However, at present, rice anther culture technology still faces many problems, such as genotype dependency, especially genotypes of indica rice.

The Fatigue Behaviors of a Medium-Carbon Pearlitic Wheel-Steel with Elongated Sulfides in High-Cycle and Very-High-Cycle Regimes.

The effects of stress ratio (), loading condition, and MnS inclusion on the fatigue behavior of a medium-carbon pearlitic wheel-steel were investigated by a combination of rotating (frequency of 52.5 Hz, 10-10) bending and ultrasonic (frequency of 20 kHz, 5 × 10-10) axial cycling tests in high-cycle and very-high-cycle regimes. All the S-N curves present horizontal asymptotic shapes and have clear fatigue limits.

Chronic stress accelerates ligature-induced periodontitis by suppressing glucocorticoid receptor-α signaling.

Periodontitis is a common chronic inflammatory disease. Recent studies have shown that chronic stress (CS) might modulate periodontal disease, but there are few models of CS-induced periodontitis, and the underlying mechanisms are unclear. The present study established a rat model of periodontitis associated with CS induced by nylon thread ligatures.

Enhancement of periodontal tissue regeneration by transplantation of osteoprotegerin-engineered periodontal ligament stem cells.

Introduction: The objective of the present study was to evaluate the capacity of a tissue-engineered complex of human osteoprotegerin (hOPG)-transfected periodontal ligament stem cells (PDLSCs) seeding on beta-tricalcium phosphate (β-TCP) to regenerate alveolar bone defects in New Zealand rabbits.

Methods: PDLSCs were isolated from rabbit periodontal ligament tissues and expanded in vitro to enrich PDLSC numbers, and their proliferative activities and differentiation capability were evaluated under specific induction conditions. Lentiviral vector containing hOPG and enhanced green fluorescent protein (EGFP) was constructed by using Gateway technology and transfected into rabbit PDLSCs.

Chronic stress enhances progression of periodontitis via α1-adrenergic signaling: a potential target for periodontal disease therapy.

This study assessed the roles of chronic stress (CS) in the stimulation of the sympathetic nervous system and explored the underlying mechanisms of periodontitis. Using an animal model of periodontitis and CS, the expression of tyrosine hydroxylase (TH) and the protein levels of the α1-adrenergic receptor (α1-AR) and β2-adrenergic receptor (β2-AR) were assessed. Furthermore, human periodontal ligament fibroblasts (HPDLFs) were stimulated with lipopolysaccharide (LPS) to mimic the process of inflammation.

[Protective effect of resveratrol on apoptosis of human periodontal ligament cells in vitro].

Objective: To investigate the effects of resveratrol (RES) on apoptosis of human periodontal ligament cells (HPLC).

Methods: HPLC were subjected to oxidative injury induced by H2O2 for 24 h after pretreatment with different concentration of RES. HPLC were then divided into the control, model, vector, RES 1, 10, 30, 50 micromol/L treatment group.