Long term administration of cyclophosphamide into MRL/1 mice. II. The effects on the isotype of anti-DNA antibodies and immunoglobulin secreting cells in the spleen.

Weekly injections of cyclophosphamide (Cy) at a dose of 20 mg/kg body weight prevented IgM to IgG class switch of serum anti-DNA antibodies and also immunoglobulin secreting cells in the spleen of MRL/Mp-lpr/lpr (MRL/1) mice. Culture experiments revealed that splenic B cells of Cy treated mice gave rise to more IgM and less IgG secreting cells than those of untreated mice in response to lipopolysaccharide. These results suggested that Cy suppressed enhanced differentiation of B cells into IgG secreting cells in MRL/1 mice, which would result in reduction of IgG anti-single stranded DNA antibodies and improvement of murine lupus like syndrome.

Long term administration of cyclophosphamide in MRL/1 mice. I. The effects on the development of immunological abnormalities and lupus nephritis.

Weekly injection of cyclophosphamide (Cy) into MRL/Mp-lpr/lpr (MRL/l) mice, at a dose of 10-20 mg/kg, from 1 month of age prevented the development of generalized lymph node enlargement, decreased serum levels of anti-DNA antibodies and immune complexes, and markedly prolonged their life span. Cy effectively suppressed enhanced differentiation of B cells, as evidenced by decreased number of immunoglobulin secreting cells in the spleen. Cy was also shown to suppress abnormal expansion of Thy-1 positive cells in lymphoid organs of MRL/1 mice.

Growth hormone response to human pancreatic growth hormone releasing factor in cattle.

The effects of a growth hormone releasing factor, human pancreatic growth hormone releasing factor-44 (hpGRF-44), on growth hormone (GH) secretion in calves, heifers and cows were studied. A single intravenous (iv) injection of 0.1, 0.

Dietary arachidonic acid supplementation increases thromboxane synthesizing activity in platelets from diabetics.

Thromboxane A2 has been implicated in thrombotic vascular diseases. The diabetic population has a high incidence of vascular complications. In the present study, activity of thromboxane synthetase in platelets of type 2 (non-insulin dependent) diabetic patients was studied.

Rectification of immunological abnormalities and lupus nephritis by the transfer of bone marrow cells.

Two-month old MRL/1 mice, which spontaneously develop lymphoproliferative syndrome and severe lupus nephritis at 4-5 months of age, were irradiated and reconstituted with bone marrow cells from a congenic strain MRL/n mice. In the chimeric (n----1) mice, the early onset of the lupus nephritis was prevented as evidenced by decrease in the degree of proteinuria, diminished intensity of immune deposits in glomeruli, and milder histopathologic changes in kidneys. Lymph node swelling as well as generation of a large number of splenic IgSC was also prevented.

Identification of immune-complex deposition and T-lymphocyte in fulminant B-viral hepatitis with immunohistochemical techniques.

Cryostat sections of the liver representing fulminant B-viral hepatitis were investigated with antisera against human immunoglobulin G(IgG), immunoglobulin M(IgM), C3, C4, C1q, hepatitis B surface antigen (HBsAg), and T-lymphocytes using the immunofluorescence and immunoperoxidase techniques. The liver showed positive staining for IgG, IgM, C3, C4, C1q, and HBsAg in the viable and necrotic hepatocytes and Kupffer cells in a granular fashion. Furthermore, the cell membrane of lymphocytes present in the liver were positively stained with anti T-lymphocyte sera.

An unusual form of vitamin D-dependent rickets in a child: alopecia and marked end-organ hyposensitivity to biologically active vitamin D.

A 12-yr-old female patient with an unusual form of vitamin D dependency and alopecia is described. She was a product of consanguineous mating and developed signs and symptoms suggesting vitamin D dependency early in life. Neither 150 microgram/day (6 microgram/kg.

Prostaglandin D2 is the major prostaglandin of arachidonic acid metabolism in rat bone marrow homogenate.

Homogenate of bone marrow of male rat was incubated with 14C-arachidonic acid and with 14C-prostaglandin H2. The major prostaglandin produced during each incubation was prostaglandin D2, which was identified by thin-layer chromatography, autoradiography and chemical reduction.

Low serum estradiol levels in subjects with arterial calcification.

The relationship between arterial calcification and serum estradiol levels was studied in 72 postmenopausal female and 59 male subjects. In both sexes, subjects with iliac artery calcification had rather lower serum estradiol levels (8.4 +/- 1.

Serum estradiol and radial mineral content in postmenopausal females.

The serum estradiol concentration and bone mineral content of the right radius were determined in 34 postmenopausal females. Regression analysis showed a significant positive correlation between the serum estradiol concentration and bone mineral content of the right radius (r=0.477, p less than 0.