Publications by authors named "Shiqi Gan"

Article Synopsis
  • Respiratory diseases in swine, caused by various viral and bacterial agents, impose significant economic impacts on the pork industry globally, highlighting the need for efficient pathogen detection methods.
  • * This research utilized a multiplex ligation-dependent probe amplification (MLPA) assay to detect seven specific respiratory pathogens in pigs, showing high sensitivity and reasonable specificity compared to existing PCR methods.
  • * Results from the study indicated that MLPA can accurately identify infections in swine, with a notable co-infection rate among the pathogens, making it a vital tool for disease management and surveillance in swine populations.
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Bacterial and fungal contamination have become major factors in fruit spoilage and damage, posing a potential risk to human health. In this work, polylactic acid (PLA) nanofibers combined with AgO-hemp fibers for a good antimicrobial effect were developed and applied to antimicrobial fruit fresh-keeping packages. The results of molecular simulation calculations showed that the strength of hydrogen bonds between AgO and hemp fibers reached 45.

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Of the three branches of unfolded protein response (UPR) that were reportedly activated by porcine epidemic diarrhea virus (PEDV), PERK is recently shown to act as an upstream regulator of oxidative response of the cells. However, it remains unknown if and how PERK activation during PEDV infection would result in oxidative stress, and whether activation of PERK and its downstream molecules affect PEDV replication. Here, we demonstrate that infection with the PEDV strain YJH/2015 triggered UPR in Vero E6 cells by activating the PERK/eIF2α pathway and led to significant increase in the expression of proapoptotic protein C/EBP homologous protein (CHOP) and ER oxidoreductase 1 alpha (ERO1α).

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