Serum metabolome associated with novel and legacy per- and polyfluoroalkyl substances exposure and thyroid cancer risk: A multi-module integrated analysis based on machine learning.

Background: Exposure to per- and polyfluoroalkyl substances (PFAS) may linked to thyroid cancer (TC) risk, but inconsistent findings and a lack of studies on mixed exposures exist, especially regarding novel PFAS compounds. Additionally, little is known about the potential mechanisms underlying the association.

Objectives: Explore the effects of PFAS exposure on the serum metabolome and its correlation with TC.

Fault detection and classification of motor bearings under multiple operating conditions.

The article presents a framework for fault detection and classification to monitor the condition of motor bearings under multiple operating conditions. The condition monitoring of motor bearings is crucial for failure prevention, as bearings are prone to failure in challenging working environments. Intelligent fault diagnosis methods driven by deep learning and model-based approaches have been widely adopted to address these concerns.

Molecular insights and functional analysis of isocitrate dehydrogenase in two gram-negative pathogenic bacteria.

Klebsiella pneumoniae and Legionella pneumophila are common Gram-negative bacteria that can cause lung infections. The multidrug resistance of K. pneumoniae presents a significant challenge for treatment.

Comparative analysis of POD genes and their expression under multiple hormones in Pyrus bretschenedri.

Background: Class III peroxidase (POD) enzymes play vital roles in plant development, hormone signaling, and stress responses. Despite extensive research on POD families in various plant species, the knowledge regarding the POD family in Chinese pear (Pyrus bretschenedri) is notably limited.

Results: We systematically characterized 113 POD family genes, designated as PbPOD1 to PbPOD113 based on their chromosomal locations.

Ethanol responsive lnc171 promotes migration and invasion of HCC cells via mir-873-5p/ZEB1 axis.

Backgrounds: Long nonconding RNAs (lncRNAs) have been found to be a vital regulatory factor in the development process of human cancer, and could regarded as diagnostic or prognostic biomarkers for human cancers. Here, we aim to confirm the expression and molecular mechanism of RP11-171K16.5 (lnc171) in hepatocellular carcinoma (HCC).

Mutability landscape guided engineering of a promiscuous microbial glycosyltransferase for regioselective synthesis of salidroside and icariside D2.

Microbial glycosyltransferases efficiently synthesize glucosides and have garnered increasing interest. However, limited regioselectivity has impeded their broad application, particularly in the pharmaceutical industry. In this study, the UDP-glycosyltransferase YjiC from Bacillus licheniformis (BlYjiC) was engineered to achieve the bidirectional regioselective glycosylation of tyrosol and its derivatives.

Vasorin promotes proliferation and migration via STAT3 signaling and acts as a promising therapeutic target of hepatocellular carcinoma.

Abnormal expression of Vasorin (VASN) is related to many types of cancer, but the signaling pathway and mechanism of how VASN contributes to the carcinogenesis of hepatocellular carcinoma (HCC) are poorly understood. Here, we found that VASN was up-regulated in serum/serum exosome and tissues of HCC patients. The expression of VASN in serum improve the detection rate of HCC in alpha-fetoprotein-negative HCC patients.

Vasorin Deletion in C57BL/6J Mice Induces Hepatocyte Autophagy through Glycogen-Mediated mTOR Regulation.

Abnormal vasorin () expression occurs in multiple diseases, particularly liver cancers. knockout (KO) in mice causes malnutrition, a shortened life span, and decreased physiological functions. However, the causes and underlying mechanisms remain unknown.

Optimised expression and purification of RBP4 and preparation of anti-RBP4 monoclonal antibody.

The expression level of retinol-binding protein 4 (RBP4) protein is closely related to liver damage and plays an important role in the diagnosis and prognosis of cancer. However, the preparation of anti-RBP4 mAb or exploration on the application of anti-RBP4 mAb has not been reported thus far. In the present study, we constructed a pET30a-RBP4 recombinant vector, used E.

Biochemical and Phylogenetic Characterization of a Novel NADP-Specific Isocitrate Dehydrogenase From the Marine Microalga .

Isocitrate dehydrogenase (IDH) family of proteins is classified into three subfamilies, namely, types I, II, and III. Although IDHs are widely distributed in bacteria, archaea, and eukaryotes, all type III IDHs reported to date are found only in prokaryotes. Herein, a novel type III IDH subfamily member from the marine microalga (PtIDH2) was overexpressed, purified, and characterized in detail for the first time.

Unveiling structural, electronic properties and chemical bonding of (VH) (n=10-30) nanoclusters: DFT investigation.

The geometries, electronic properties, and chemical bonding of (VH) (n=10-30) nanoclusters are systematically investigated by a combination of artificial bee colony optimization with density functional theory calculations. Structure analysis indicates that the structures of (VH) nanoclusters tend to be a disorder, where the hydrogen atoms prefer to occupy the hollow sites among different V atoms, binding three V atoms to form the HV moieties. The bond length suggests that the average V-V bond lengths are about 2.

Long noncoding RNA MNX1-AS1 functions as a competing endogenous RNA to regulate epithelial-mesenchymal transition by sponging MiR-744-5p in colorectal cancer.

Colorectal cancer (CRC) is the fourth most deadly cancer globally. Long noncoding RNA MNX1-AS1 has been proven to play a regulatory role in various human cancers. The present research aimed to explore the MNX1-AS1 function in CRC and the corresponding mechanism.

Sum of fears among intraguild predators drives the survival of green sea turtle () eggs.

Ecologists have long theorized that apex predators stabilize trophic systems by exerting a net protective effect on the basal resource of a food web. Although experimental and observational studies have borne this out, it is not always clear what behavioural mechanisms among the trophically connected species are responsible for this stability. Fear of intraguild predation is commonly identified as one such mechanism in models and mesocosm studies, but empirical evidence in natural systems remains limited, as the complexity of many trophic systems renders detailed behavioural studies of species interactions challenging.

The opposing roles of the mTOR signaling pathway in different phases of human umbilical cord blood-derived CD34 cell erythropoiesis.

As an indispensable, even lifesaving practice, red blood cell (RBC) transfusion is challenging due to several issues, including supply shortage, immune incompatibility, and blood-borne infections since donated blood is the only source of RBCs. Although large-scale in vitro production of functional RBCs from human stem cells is a promising alternative, so far, no such system has been reported to produce clinically transfusable RBCs due to the poor understanding of mechanisms of human erythropoiesis, which is essential for the optimization of in vitro erythrocyte generation system. We previously reported that inhibition of mammalian target of rapamycin (mTOR) signaling significantly decreased the percentage of erythroid progenitor cells in the bone marrow of wild-type mice.

Biochemical Characterization and Crystal Structure of a Novel NAD-Dependent Isocitrate Dehydrogenase from .

The marine diatom originated from a series of secondary symbiotic events and has been used as a model organism for studying diatom biology. A novel type II homodimeric isocitrate dehydrogenase from (PtIDH1) was expressed, purified, and identified in detail through enzymatic characterization. Kinetic analysis showed that PtIDH1 is NAD-dependent and has no detectable activity with NADP.

Tackling the Activity and Selectivity Challenges of Electrocatalysts toward the Nitrogen Reduction Reaction via Atomically Dispersed Biatom Catalysts.

Developing efficient catalysts for nitrogen fixation is becoming increasingly important but is still challenging due to the lack of robust design criteria for tackling the activity and selectivity problems, especially for electrochemical nitrogen reduction reaction (NRR). Herein, by means of large-scale density functional theory (DFT) computations, we reported a descriptor-based design principle to explore the large composition space of two-dimensional (2D) biatom catalysts (BACs), namely, metal dimers supported on 2D expanded phthalocyanine (M-Pc or MM'-Pc), toward the NRR at the acid conditions. We sampled both homonuclear (M-Pc) and heteronuclear (MM'-Pc) BACs and constructed the activity map of BACs by using NH* adsorption energy as the activity descriptor, which reduces the number of promising catalyst candidates from over 900 to less than 100.

Biochemical and phylogenetic characterization of a monomeric isocitrate dehydrogenase from a marine methanogenic archaeon Methanococcoides methylutens.

Monomeric isocitrate dehydrogenase (IDH) stands for a separated subgroup among IDH protein family. Up to now, all reported monomeric IDHs are from prokaryotes. Here, a monomeric IDH from a marine methanogenic archaeon Methanococcoides methylutens (MmIDH) was reported for the first time.

An Improved Calibration Technique for MEMS Accelerometer-Based Inclinometers.

Micro-electro-mechanical system (MEMS) accelerometer-based inclinometers are widelyused to measure deformations of civil structures. To further improve the measurement accuracy, anew calibration technique was proposed in this paper. First, a single-parameter calibration modelwas constructed to obtain accurate angles.

An Accurate Image Measurement Method Based on a Laser-Based Virtual Scale.

Image measurement methods have been widely used in broad areas due to their accuracy and efficiency. However, current techniques usually involve complex calibration, an elaborate optical design, or sensitivity to the test environment. In this paper, a simple optical device was designed to emit parallel beams to obtain a virtual scale for measurement purposes.

Comparison Among Five Eucalyptus Species Based on Their Leaf Contents of Some Primary and Secondary Metabolites.

Background: Eucalyptus belongs to the Myrtaceae family. It is the most planted hardwood forest crop worldwide, representing a global renewable resource of fiber, pharmaceuticals and energy.

Objective: To compare the five species, E.

A New Model for Optimal Mechanical and Thermal Performance of Cement-Based Partition Wall.

The prefabricated cement-based partition wall has been widely used in assembled buildings because of its high manufacturing efficiency, high-quality surface, and simple and convenient construction process. In this paper, a general porous partition wall that is made from cement-based materials was proposed to meet the optimal mechanical and thermal performance during transportation, construction and its service life. The porosity of the proposed partition wall is formed by elliptic-cylinder-type cavities.

Heteroexpression and biochemical characterization of a glucose-6-phosphate dehydrogenase from oleaginous yeast Yarrowia lipolytica.

Yarrowia lipolytica, a nonpathogenic, nonconventional, aerobic and dimorphic yeast, is considered an oleaginous microorganism due to its excellent ability to accumulate large amounts of lipids. Glucose-6-phosphate dehydrogenase (G6PD) is one of two key enzymes involved in the lipid accumulation in this fungi, which catalyzes the oxidative dehydrogenation of glucose-6-phosphate to 6-phosphoglucono-δ-lactone with the reduction of NADP to NADPH. In this study, the full-length gene of G6PD from Y.

Comparative effects of some extraction solvents on the antimicrobial activity of leaf, bud, capsule and seed crude extracts.

Well diffusion method was used to evaluate the antibacterial activity of , while the antifungal effect was assessed by calculating the reduction percent in the radial growth of mycelia. The inhibition zones exerted by crude extracts varied significantly ( ≤ 0.01).

A Practical, Robust and Fast Method for Location Localization in Range-Based Systems.

Location localization technology is used in a number of industrial and civil applications. Real time location localization accuracy is highly dependent on the quality of the distance measurements and efficiency of solving the localization equations. In this paper, we provide a novel approach to solve the nonlinear localization equations efficiently and simultaneously eliminate the bad measurement data in range-based systems.