Fabrication and properties of temperature-responsive imprinted sensors based on fluorescently labeled yeast cells MVL ATRP.

Temperature-responsive yeast cell-imprinted sensors (CIPs/AuNPs/TiCT/AuNPs/Au) were prepared based on fluorescein isothiocyanate labeled yeast cells (FITC-yeast) metal-free visible-light-induced atom transfer radical polymerization (MVL ATRP). Here, -isopropyl acrylamide (NIPAM) was used as a temperature-responsive functional monomer, α-methacrylic acid (MAA) was chosen as an auxiliary functional monomer, ,'-methylene bisacrylamide (MBA) was used as a cross-linker, and FITC-yeast was selected as both a template and photocatalyst. Under the optimal conditions, the detection range of the yeast cell-imprinted sensor toward yeast cells was 1.

Ultramicro and ultrasensitive detection of lipopolysaccharides based on triple-signal amplification ultrafast ATRP and an ultramicroelectrode.

Highly sensitive testing of trace lipopolysaccharides (LPS) is very important due to their high toxicity to the human body. Here, an ultrasensitive electrochemical sensor requiring only 5 μL solution was developed for LPS detection triple-signal amplification based on ultrafast atom transfer radical polymerization (UATRP) and a Au ultramicroelectrode (UME). Firstly, the Au UME was modified with gold nanoparticles (nAu) and an LPS aptamer (Apt) in turn.

Molecular characterization and functional analysis of cyp11a and cyp11b in black rockfish (Sebastes schlegelii).

The cyp11 includes cyp11a and cyp11b in most mammals and teleosts, encoded cholesterol side chain lyase and 11β-hydroxylase, respectively. It is essential in steroid hormone synthesis. However, studies on the regulation of cyp11 are limited, especially in teleosts.

[Experimental studies of rhizoma Astilbes chinensis on its effects in abirritation, blood activation, cough relieving and sputum elimination].

Objective: To study the effects of rhizoma Astilbes chinensis in abirritation, blood activation, cough relieving and sputum elimination.

Method: The antalgic function of rhizoma A. chinensis was tested by hot-plate method and writhing reaction.