Innovative quantum dots-based SERS for ultrasensitive reporting of contaminants in food: Fundamental concepts and practical implementations.

Food contamination poses serious health risks, compelling the discovery of new methods to guarantee regulatory compliance and build consumer conviction. Surface Enhanced Raman Spectroscopy (SERS) has come into sight as a sophisticated approach for the ultrasensitive discovery of toxins in food and water, proposing non-destructive, quick, and precise analysis. Instantaneously, quantum dots (QDs) are astonishing nanomaterials, characterized by distinctive attributes such as quantum confinement and optical photostability.

Magnetic metal-organic frameworks-based ratiometric SERS aptasensor for sensitive detection of patulin in apples.

Patulin (PAT), a major contaminant in apples, poses a considerable food safety risk, necessitating a rapid, sensitive and reliable detection method. This study developed a novel magnetic metal-organic frameworks (MOFs)-based ratiometric surface-enhanced Raman scattering (SERS) aptasensor. The sensor consists of magnetic MOFs loaded with 4-Mercaptobenzoic acid (4-MBA) internal labeled Au@Ag as the SERS substrate, and gold nanorods (AuNRs) modified with Rhodamine 6G and aptamers as capture probes.

Ultrasensitive Analysis of O157:H7 Based on Immunomagnetic Separation and Labeled Surface-Enhanced Raman Scattering with Minimized False Positive Identifications.

It is a big challenge to monitor pathogens in food with high selectivity. In this study, we reported an ultrasensitive method for O157:H7 detection based on immunomagnetic separation and labeled surface-enhanced Raman scattering (SERS). The bacterium was identified by heterogeneous recognition elements, monoclonal antibody (mAb), and aptamer.

Association between Bisphenol A and Prostate-Specific Antigen (PSA) among U.S. Older Males: National Health and Nutrition Examination Survey (NHANES), 2003-2012.

Background: There is growing evidence indicating that environmental endocrine disruptors may influence the development of prostate cancer. Despite this, the connection between BPA and PSA levels is still not fully understood and appears intricate. In this study, we aimed to assess the link between BPA exposure and PSA levels using data from the NHANES database.

Expanding the CRISPR Toolbox for Engineering Lycopene Biosynthesis in .

Lycopene represents one of the central compounds in the carotenoid pathway and it exhibits a potent antioxidant ability with wide potential applications in medicine, food, and cosmetics. The microbial production of lycopene has received increasing concern in recent years. () is considered to be a safe and beneficial industrial production platform, naturally endowed with the ability to produce lycopene.

Recent and emerging trends of metal-organic frameworks (MOFs)-based sensors for detecting food contaminants: A critical and comprehensive review.

Interest in the use of sensors based on metal-organic frameworks (MOFs) to detect food pollutants has been growing recently due to the desirable characteristics of MOFs, including uniform structures, large surface area, ultrahigh porosity and easy-to-functionalize surface. Fundamentally, this review offers an excellent solution using MOFs-based sensors (e.g.

Magnetic nanoparticle-based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update.

Mycotoxin contamination of food crops is a global challenge due to their unpredictable occurrence and severe adverse health effects on humans. Therefore, it is of great importance to develop effective tools to prevent the accumulation of mycotoxins through the food chain. The use of magnetic nanoparticle (MNP)-assisted biosensors for detecting mycotoxin in complex foodstuffs has garnered great interest due to the significantly enhanced sensitivity and accuracy.

A film-like SERS aptasensor for sensitive detection of patulin based on GO@Au nanosheets.

Patulin (PAT) commonly contaminates fruits, posing a significant risk to human health. Therefore, a highly effective and sensitive approach in identifying PAT is warranted. Herein, a SERS aptasensor was constructed based on a two-dimensional film-like structure.

Recent advances in electrochemical cell-based biosensors for food analysis: Strategies for sensor construction.

Owing to their advantages such as great specificity, sensitivity, rapidity, and possibility of noninvasive and real-time monitoring, electrochemical cell-based biosensors (ECBBs) have been a powerful tool for food analysis encompassing the areas of nutrition, flavor, and safety. Notably, the distinctive biological relevance of ECBBs enables them to mimic physiological environments and reflect cellular behaviors, leading to valuable insights into the biological function of target components in food. Compared with previous reviews, this review fills the current gap in the narrative of ECBB construction strategies.

Significance of the antibody orientation for the lateral flow immunoassays: A mini-review.

Lateral flow immunoassays (LFIAs) are well-established and broadly commercialized tools in the field of point-of-care testing due to their simplicity, rapidity, cost-effectiveness, and low requirements for users and equipment. However, the insensitivity and the possibility of producing inaccurate results associated with conventional LFIAs have impeded their wide-ranging implementation, especially for monitoring ultra-trace level of analytes. Moreover, the heterogeneous distribution of amino acids on the surface of antibody (Ab) results in a lack of precise control over their orientation, which ultimately leads to unsatisfactory detection performance.

Dextran-coated nanoparticles as immunosensing platforms: Consideration of polyaldehyde density, nanoparticle size and functionality.

Magnetic nanoparticles (MNPs) can be used as antibody carriers in a wide range of immunosensing applications. The conjugation chemistry for preparing antibody-MNP bionanohybrids should assure the nanoparticle's colloidal dispersity, directional conformation and high biofunctionality retention of attached antibodies. In this work, peroxidase (HRP) was selected as model target analyte, and stable antibody-MNP conjugates were prepared using polyaldehyde-dextrans as multivalent linkers, also to prevent nanoparticles agglomeration and steric shielding of non-specific proteins.

Oriented immobilization of antibodies onto sensing platforms - A critical review.

The immunosensor has been proven a versatile tool to detect various analytes, such as food contaminants, pathogenic bacteria, antibiotics and biomarkers related to cancer. To fabricate robust and reproducible immunosensors with high sensitivity, the covalent immobilization of immunoglobulins (IgGs) in a site-specific manner contributes to better performance. Instead of the random IgG orientations result from the direct yet non-selective immobilization techniques, this review for the first time introduces the advances of stepwise yet site-selective conjugation strategies to give better biosensing efficiency.

Oriented immobilization of antibodies through different surface regions containing amino groups: Selective immobilization through the bottom of the Fc region.

Amino groups on the antibody surface (amino terminus and Lys) are very interesting conjugation targets due to their substantial quantities and selectivity toward various reactive groups. Oriented immobilization of antibodies via amino moieties on the Fc region instead of the antigen-binding fragment (Fab) is highly appreciated to conserve antigen-binding capacity. In this paper, targeting amino moieties on distinct regions, three antibody immobilization strategies were compared with the recognition ability of corresponding adsorbents.

Endoglucanase enzymatic modification of kraft pulp during recycling.

Objective: To investigate the cellulose modification process on kraft pulp during recycling by mono-endoglucanase.

Results: Pichia pastoris expressing endoglucanase, EG1, was grown in a 10 l fermenter yielding a high carboxymethyl cellulase (CMCase) activity of 340 U mg(-1). EG1-mediated modification of kraft pulp resulted in a paper sheet with the tensile index and burst index increased by 10 and 6.