A Study of the Deisotope Method for Mass Spectra of Complex Synthetic Polymer Mixtures Consisting of Multiple Repeating Units.

In the interpretation step of mass spectra obtained from synthetic polymers, isotope peaks are typically intense and cannot be ignored, especially in the higher mass range. To reduce the complexity of the spectra, deisotope processing is used. In this study, a deisotope processing method that is effective even for mixtures of different types of polymers is investigated.

Chemoenzymatic Synthesis of Sialyl Sulfo-Oligosaccharides as Potent Siglec-8 Ligands via Transglycosylation Catalyzed by Keratanase II.

Sialyl type-II sulfo-oligosaccharides are gaining much attention as bioactive ligands for Siglecs. In this study, we have achieved the first synthesis of sialyl type-II sulfo-oligosaccharides chemoenzymatically by utilizing the transglycosylation activity of keratanase II. The oxazoline derivative of α(2→3)-sialylated 6,6'-di-sulfo-LacNAc () was newly designed as the glycosyl donor for enzymatic transglycosylation.