Publications by authors named "Shiori Oguro"
Novel antigenic variant strains of the infectious bursal disease virus (IBDV) classified into genogroup A2d have been found in the western part of Japan since 2017. Novel antigenic variant IBDVs now occur in higher frequencies in poultry houses and have been detected in the eastern part of Japan, indicating the spread of IBDVs despite the usual IBDV vaccination. We isolated a novel antigenic variant IBDV, designated as the B2977CE2C3 strain.
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- Avian infectious bronchitis virus (IBV) affects chickens, leading to serious respiratory and reproductive issues, complicated by evolving viral variants that challenge vaccine effectiveness.
- This study developed a reverse genetics system (RGS) utilizing bacterial artificial chromosomes (BAC) to clone the full-length cDNA of the avirulent IBV C-78E128 strain for further research and vaccine development.
- Through genetic manipulation, researchers replaced the S gene of the C-78E128 strain with that from a virulent strain, finding that while the recombinant virus was avirulent and induced specific antibodies, it did not replicate the tissue tropism of the virulent strain, demonstrating that the S gene affects immunogenicity but not tissue preference or pathogenicity
Genotyping of avian infectious bronchitis virus (IBV) was performed on trachea and kidney samples of six chickens obtained from a single farm in Japan. Using two primer sets targeting the spike (S) protein gene, the S1 and S2 regions of DNA fragments were amplified. Sequences of amplified S1 fragments extracted from both organs were identical among the six chickens, showing a JP-I genotype.
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