[Clinical application of arthroscopy on temporomandibular joint].

We present the anatomy, methodology, procedure for arthroscopy on temporomandibular joint. Arthroscopic view of normal and pathologic view on superior articular cavity of temporomandibular joint are presented. There were no serious complications except slight swelling on temporomandibular joint.

Co-existence of TRH with mesotocin in the same axon terminals of the bullfrog pars nervosa as revealed by double labeling immunocytochemistry.

The co-existence of thyrotropin-releasing hormone (TRH)- and mesotocin (MT)-like immunoreactivities in single axon terminals in the frog pars nervosa was observed using a method combining pre-embedding peroxidase-antiperoxidase for TRH with post-embedding immunocolloidal gold staining for MT. Both TRH- and MT-like immunoreactivities were localized in the same large dense elementary granules 130-220 nm in diameter (170 nm mean diameter). A few axons contained only TRH-like immunoreactive small granular vesicles 80-120 nm in diameter (92 nm mean diameter).

[Reconsideration of the bilaminar zone in the retrodiscal connective tissue of the TMJ. 2. Fibrous structure of the retrodiscal connective tissue and relation between those fibers and the disk].

The fibrous structure of the retrodiscal connective tissue was investigated histologically in order to reconfirm what Rees calls the bilaminar zone. Eleven TMJs were taken from the fresh cadavers. The sections, obtained from six materials with condyle protruded, were observed mainly.

[Reconsideration of the bilaminar zone in the retrodiscal connective tissue of the TMJ. 1. Relation between discal fiber and the condyle and components around the disk].

The fibrous connection of the discal attachment to the condyle and to the other structures around the disk was investigated histologically in order to reconfirm what Rees calls the bilaminar zone. Eleven TMJs were taken from fresh cadavers. Sections, obtained from six materials with condyle protruded, were observed.

Thyrotropin-releasing hormone-like immunoreactive neurons in rabbit medulla oblongata.

Thyrotropin-releasing hormone-like immunoreactive (TRH-LI) neuronal cell bodies and processes were identified by using the peroxidase-antiperoxidase method in the medullar oblongata of rabbits. TRH-LI cell bodies were mainly distributed in the ventral medulla (paraolivary and parapyramidal regions), and caudal raphe nuclei (nucleus raphe obscurus and nucleus raphe pallidus). TRH-LI processes with varicosities were densely distributed in the solitary nucleus, dorsal motor nucleus of the vagus nerve and area postrema.

Response of the mouse femoral muscle to an implant of a composite of bone morphogenetic protein and plaster of Paris.

A composite consisting of 1 mg of bone morphogenetic protein (BMP) and 5 mg of plaster of Paris (PLP) was implanted into the mouse femoral muscle. Control PLP without BMP was implanted into the contralateral muscle. The BMP/PLP composite induced cartilage formation within two weeks, trabecular bone within three weeks, and lamellar bone including bone marrow within six weeks.

Electron microscopic studies of primary culture cells from ameloblastoma.

Primary culture cells from eight cases of ameloblastoma were obtained and observed by means of light microscopy. Cells in four cases were observed by means of electron microscopy. Cultured cells in all cases resembled epithelial cells with the cells from follicular ameloblastoma more tightly packed than those from the plexiform types.

[Results of surgical treatment of tongue cancer].

Fourty-four patients with squamous cell carcinoma of the tongue have been treated by surgery. Twenty-two out of 44 patients were treated by a glossectomy alone and the remaining 22 by en bloc dissection with a glossectomy and neck dissection. The 5-year cumulative survival rate of these 44 cases was 86.

Innervation of thyrotropin-releasing hormone-containing neurons in the bullfrog pars intermedia.

We studied immunocytochemical localization of thyrotropin-releasing (TRH) in the bullfrog pats intermedia. Many TRH-like immunoreactive terminals containing immunoreactive granular vesicles 80-150 nm in diameter (116 nm mean diameter) were found in the pars intermedia. Some TRH-like immunoreactive terminals were in synaptic contact with the intermedia cells.

TRH axon terminals in synapsis with GRF neurons in the arcuate nucleus of the rat hypothalamus as revealed by double labeling immunocytochemistry.

Synaptic input to neurons containing growth hormone-releasing factor (GRF) by axon terminals containing thyrotropin-releasing hormone (TRH) in the arcuate nucleus (AN) of the rat hypothalamus was examined using a method combining pre-embedding peroxidase-anti-peroxidase for GRF with postembedding immunocolloidal gold staining for TRH. The TRH-like immunoreactive axon terminals were found to make synaptic contact with GRF-like immunoreactive neurons with unlabeled axon terminals. From these findings, TRH-containing neurons in the hypothalamic AN of the rat may be considered to innervate GRF neurons, to regulate GRF secretion or to have some other functions via synapses.

Catecholaminergic innervation of neurons containing corticotropin-releasing factor in the paraventricular nucleus of the rat hypothalamus.

Catecholaminergic synaptic input to neurons containing corticotropin-releasing factor (CRF) in the parvocellular portion of the paraventricular nucleus (PVN) in the rat hypothalamus was observed. The experimental techniques used combine autoradiography after 3H-noradrenaline (3H-NA) injection or uptake of 5-hydroxydopamine (5-OHDA) with immunocytochemistry using CRF antiserum. CRF-like immunoreactive cell bodies and fibers in the PVN received synaptic inputs from the axon terminals in which a selective accumulation of 3H-NA or 5-OHDA was found.

[Carcinoma of the oral cavity].

The diagnosis and treatment of carcinoma of the oral cavity have improved with the recent progress of medical science, further enhancing the recovery of oral function and therapeutic results. These processes are described with special reference to primary cases of carcinoma of the oral cavity (squamous cell carcinoma) examined in our department during the past eight-year period.

Electron-microscopic cytochemistry of the catecholaminergic innervation of TRH neurons in the rat hypothalamus.

The catecholaminergic innervation of thyrotropin-releasing hormone (TRH) neurons was examined by use of a combined method of 5-hydroxydopamine (5-OHDA) uptake or autoradiography after intraventricular injection of 3H-noradrenaline (3H-NA) and immunocytochemistry for TRH in the same tissue sections at the electron-microscopic level. TRH-like immunoreactive nerve cell bodies were distributed abundantly in the parvocellular part of the paraventricular nucleus (PVN), in the suprachiasmatic preoptic nucleus and in the dorsomedial nucleus of the rat hypothalamus. In the PVN, a large number of immunonegative axon terminals were found to make synaptic contact with TRH-like immunoreactive cell bodies and fibers.

A study of the clinical characteristics and treatment of oral carcinoma in situ.

Twelve patients with oral carcinoma in situ were examined to clarify the clinical features and the most suitable therapy for this lesion. Seven cases were classified clinically as the erythroplakic type, two as the leukoplakic type, and three as the mixed type. The cumulative survival rate of ten cases, excluding two cases with lesions of the soft palate, was 100% in 10 years.

Surface roughness of squamous cell carcinoma of the oral mucosa.

The surface roughness value of 39 oral squamous cell carcinomas was measured by the Rmax method. The means of the eight measurement values of each carcinoma ranged from 87 micron to 1176 micron. The average value of all 39 lesions was 341 micron.

[Long-term prognosis of oral squamous cell carcinoma histologically judged to have been eliminated by the treatment with bleomycin alone].

This study was intended to reveal the long-term prognosis in 21 patients with oral squamous cell carcinoma evaluated histologically as having disappeared by bleomycin treatment. Of 16 cases treated with bleomycin alone, recurrence was observed in 4, and cervical metastases in 2, with survival achieved for 5 years or more in 7 (more than 10 years in 3). No recurrence was observed in 5 patients in whom surgery was additionally performed after the bleomycin treatment, but cervical metastasis was found in one of them.

Immunocytochemical observations of corticotropin-releasing-factor-containing neurons in the rat hypothalamus with special reference to neuronal communication.

Synapses between neurons with corticotropin-releasing-factor-(CRF)-like immunoreactivities and other immunonegative neurons in the hypothalamus of colchicine-treated rats, especially in the paraventricular nucleus (PVN) and the supraoptic nucleus (SON) were observed by immunocytochemistry using CRF antiserum. The immunoreactive nerve cell bodies and fibers were numerous in both the PVN and the SON. The CRF-containing neurons had synaptic contacts with immunonegative axon terminals containing a large number of clear synaptic vesicles alone or combined with a few dense-cored vesicles.