Characterization of a Novel -3-Hydroxy-l-Proline Dehydratase and a -3-Hydroxy-l-Proline Dehydratase from Bacteria.

Hydroxyprolines, such as -4-hydroxy-l-proline (T4LHyp), -3-hydroxy-l-proline (T3LHyp), and -3-hydroxy-l-proline (C3LHyp), are present in some proteins including collagen, plant cell wall, and several peptide antibiotics. In bacteria, genes involved in the degradation of hydroxyproline are often clustered on the genome (l-Hyp gene cluster). We recently reported that an aconitase X (AcnX)-like gene from an l-Hyp gene cluster functions as a monomeric C3LHyp dehydratase (AcnX).