Publications by authors named "Shinri Fujimoto"
Article Synopsis
- * VPA treatment increased levels of HS and related enzymes, and the inhibition of HS synthesis reduced the effectiveness of ASCs differentiating into neurons.
- * The addition of HS donors, like GYY4137, alongside cAMP-elevating agents significantly boosted neuronal differentiation and was linked to calcium entry and increased expression of calcium channels and NO-cycle enzymes, suggesting HS contributes to the neuronal differentiation alongside VPA.
Article Synopsis
- Valproic acid (VPA) significantly enhances the differentiation of adipose tissue-derived stem cells (ASCs) into neuronal cells, especially when followed by a treatment with neuronal induction medium (NIM).
- Treatment with VPA for 3 days and NIM for 2 hours resulted in a higher incidence of neuronal cell differentiation compared to 6 days of VPA alone, indicating a more efficient process.
- The study found that VPA increases intracellular calcium levels and the expression of certain calcium channel mRNAs, suggesting that its interaction with NIM may facilitate important signaling pathways for neuronal development, potentially aiding in therapies for neurological disorders related to calcium channel dysfunction.
Valproic acid (VPA) remarkably promotes the differentiation of adipose tissue-derived stem cells (ASCs) to mature neuronal cells, enabling neuronal induction within only three days. Here, we investigated the involvement of NO-signaling in the VPA-promoted neuronal differentiation of ASCs as a possible mechanism. Cultured rat ASCs were differentiated to matured neuronal cells rich in dendrites and expressing βIII-tubulin protein, a neuronal marker, by treatments with VPA at 2 mM for 3 days and subsequently with the neuronal induction medium (NIM) containing cAMP-elevating agents for 2 h.
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