Synopsis of recent research by authors named "Shino Tsuzuki"
- Shino Tsuzuki's research primarily focuses on enzyme engineering, specifically the characterization and modification of d-lactate dehydrogenase from Lactobacillus pentosus to enhance its substrate specificity and activity.
- One key finding highlights that a single amino acid substitution, such as replacing Tyr52 with amino acids like Leu, Val, or Ala, can significantly boost the enzyme's activity towards larger aliphatic 2-ketoacid substrates while altering its preference for smaller substrates like pyruvate.
- Tsuzuki's work indicates that certain amino acid replacements can lead to substantial improvements in enzymatic efficiency, demonstrating the potential for targeted modifications in enzyme function through specific genetic alterations.
