Isolation and sequencing of swine carbonic anhydrase VI, an enzyme expressed in the swine kidney.

Background: Carbonic anhydrase VI (CA-VI) is produced by the salivary gland and is secreted into the saliva. Although CA-VI is found in the epithelial cells of distal straight tubule of swine kidneys, the exact function of CA-VI in the kidneys remains unclear.

Results: CA-VI was located in the epithelial cells of distal straight tubule of swine kidneys.

Determination of full-length cDNA nucleotide sequence of equine carbonic anhydrase VI and its expression in various tissues.

A full-length cDNA clone of an equine carbonic anhydrase (CA)-VI was obtained from the equine parotid gland. The cDNA sequence was 1338 bp long and was predicted to encode a 319 amino acid polypeptide with a putative signal peptide of 18 amino acids. The deduced amino acid sequence of mature CA-VI showed the similarity of 70% to those of other mammalians reported.