Functionalized magnetic nanoparticles as an in vivo delivery system.

We developed extremely small functionalized magnetic nanoparticles (MNPs) for use as an in vivo delivery system for pharmaceuticals and biomolecules. We functionalized the MNPs (d = 3 nm) by silanization of amino groups on the particles with (3-aminopropyl)triethoxysilane for subsequent cross-linking with pharmaceuticals and biomolecules. The MNPs were successfully introduced into living cells without any further modification, such as the use of cationic residues, to enhance endocytic internalization.

Magnetic nanoparticle-based mass spectrometry for the detection of biomolecules in cultured cells.

We prepared functionalized magnetic nanoparticles (d = 3.5 nm) to serve as a laser desorption/ionization material for mass spectrometry. The obtained nanoparticles consisteds of a macaulayite core conjugated with hydroxyl and amino groups, and showed superparamagnetism at room temperature.

Nanoparticle-assisted laser desorption/ionization based mass imaging with cellular resolution.

Today, two-dimensional mass spectrometry analysis of biological tissues by means of a technique called mass imaging, mass spectrometry imaging (MSI), or imaging mass spectrometry (IMS) has found application in investigating the distribution of moleculesMSI with matrix-assisted laser desorption/ionization (MALDI) and secondary ion MS (SIMS). However, the size of the matrix crystal and the migration of analytes can decrease the spatial resolution in MALDI, and SIMS can only ionize compounds with relatively low molecular weights. To overcome these problems, we developed a nanoparticle-assisted laser desorption/ionization (nano-PALDI)-based MSI.

Functionalized nano-magnetic particles for an in vivo delivery system.

Nanotechnologies to allow the nondisruptive introduction of carriers in vivo have wide potential for therapeutic delivery system. We have prepared functional nano-magnetic particles (d = 3 nm) by silanization with (3-aminopropyl) triethoxysilane. For the purpose of functionalizing the surface of the nanoparticles with amino groups for subsequent cross-linking with pharmaceuticals and biomolecules.