Development of INDEL markers to discriminate all genome types rapidly in the genus Oryza.

The wild Oryza species are rich in genetic diversity and are good resources for modern breeding of rice varieties. The reliable ex situ conservation of various genetic resources supports both basic and applied rice research. For this purpose, we developed PCR-based and co-dominant insertion/deletion (INDEL) markers which enable the discrimination of the genome types or species in the genus Oryza.

Ovule is a lateral organ finally differentiated from the terminating floral meristem in rice.

The floral meristem is the homeostatic source of floral organs in angiosperms. In rice, after stamen and carpel differentiation, the floral meristem is terminated and exhausted to produce an ovule. To elucidate underlying mechanisms controlling the continuous process from floral meristem termination to ovule formation, we investigated two rice mutants showing abnormalities in ovule formation.

Rice expression atlas in reproductive development.

Gene expression throughout the reproductive process in rice (Oryza sativa) beginning with primordia development through pollination/fertilization to zygote formation was analyzed. We analyzed 25 stages/organs of rice reproductive development including early microsporogenesis stages with 57,381 probe sets, and identified around 26,000 expressed probe sets in each stage. Fine dissection of 25 reproductive stages/organs combined with detailed microarray profiling revealed dramatic, coordinated and finely tuned changes in gene expression.

Rice plant development: from zygote to spikelet.

Rice is becoming a model plant in monocotyledons and a model cereal crop. For better understanding of the rice plant, it is essential to elucidate the developmental programs of the life cycle. To date, several attempts have been made in rice to categorize the developmental processes of some organs into substages.