Comprehensive Optimization of Interferometric Diffusing Wave Spectroscopy (iDWS).

It has been shown that light speckle fluctuations provide a means for noninvasive measurements of cerebral blood flow index (CBFi). While conventional Diffuse Correlation Spectroscopy (DCS) provides marginal brain sensitivity for CBFi in adult humans, new techniques have recently emerged to improve diffuse light throughput and thus, brain sensitivity. Here we further optimize one such approach, interferometric diffusing wave spectroscopy (iDWS), with respect to number of independent channels, camera duty cycle and full well capacity, incident power, noise and artifact mitigation, and data processing.

Time-Multiplexed Miniaturized Two-Photon Microscopy.

We propose a time-multiplexed miniaturized two-photon microscope (TM-MINI2P), enabling a two-fold increase in imaging speed while maintaining a high spatial resolution. Using TM-MINI2P, we conducted high-speed in-vivo calcium imaging in mouse cortex.

Simultaneous Dual-region Functional Imaging in Miniaturized Two-photon Microscopy.

We demonstrate simultaneous dual-region in-vivo imaging of brain activity in mouse cortex through a miniaturized spatial-multiplexed two-photon microscope platform, which doubles the imaging speed. Neuronal signals from the two regions are computationally demixed and extracted.

Recovering fetal signals transabdominally through interferometric near-infrared spectroscopy (iNIRS).

Noninvasive transabdominal fetal pulse oximetry can provide clinicians critical assessment of fetal health and potentially contribute to improved management of childbirth. Conventional pulse oximetry through continuous wave (CW) light has challenges measuring the signals from deep tissue and separating the weak fetal signal from the strong maternal signal. Here, we propose a new approach for transabdominal fetal pulse oximetry through interferometric near-infrared spectroscopy (iNIRS).

High Speed Miniaturized Multiphoton Microscopy with Elliptical Beam Excitation.

We demonstrate elliptical beam shaping in a miniaturized two-photon microscope enabling cellular resolution fluorescence imaging at over three times faster frame rate than point scanning over a 400 μm diameter FOV.