Quantification of PrPC in bovine peripheral tissues: Analysis in wild-type and PrPC-deficient cattle.

Cellular PrP (PrPC) is necessary for bovine spongiform encephalopathy (BSE) infection. The purpose of the present experiment was the quantification of PrPC in peripheral tissues to assess the risk of BSE infection from these tissues. The tissue distribution of PrPC was examined by a sandwich enzyme-linked immunosorbent assay (sELISA) and histochemical analysis.

Ratio of peripheral nervous tissues in tongues, skeletal muscles and intestines in cows.

It is suggested that pathogenic prion protein (PrP(sc) may accumulate in peripheral nervous tissues in addition to the specified-risk materials. To assess the risk of PrP(sc) accumulation in peripheral tissues, the present study was designed to study the ratio of peripheral nervous tissues in tongues, skeletal muscles and intestines in 2-week- and 59-month-old cows. The nervous tissues could be detected by immunohistochemistry using antibody Neurofilament 150.

A new type of O(2)(-)-generating tool for oxidative stress studies by remodeling neutrophil NADPH oxidase.

The effects of reactive oxygen species on cells have attracted much attention in relation to redox regulation and oxidative stress-related diseases. Superoxide (O(2)(-)) is the reactive oxygen species primarily formed in biological systems. However, no convenient O(2)(-)-generating device has been available for use in cell or tissue culture.