Publications by authors named "Shin-ichi Fujimaki"
Article Synopsis
- Acquired von Willebrand syndrome (AVWS) is linked to cardiovascular diseases like mitral regurgitation (MR), leading to a decrease in large von Willebrand factor (VWF) multimers, but its specifics with MR are still not fully understood.
- A study analyzed 84 patients with moderate to severe MR, finding that 69% exhibited a significant loss of VWF large multimers, especially in degenerative MR cases, but levels improved after mitral valve intervention.
- The findings indicate that while MR is commonly associated with loss of VWF large multimers, the overall risk of gastrointestinal bleeding remains low, and hemoglobin levels tend to stay stable post-treatment.
Article Synopsis
- Severe aortic stenosis (AS) can lead to acquired von Willebrand syndrome by breaking down important blood clotting factors, requiring accurate diagnosis methods to identify the condition.* -
- The study evaluated the effectiveness of the VWF Ristocetin co-factor activity to antigen levels (VWF:RCo/VWF:Ag) ratio as a diagnostic tool for AS-induced von Willebrand syndrome using data from 382 AS patients and controls.* -
- Results showed a VWF:RCo/VWF:Ag ratio of <0.7 is specific for detecting loss of important blood clotting multimers in patients with AS, but it has low sensitivity, indicating it might miss some cases.*
Background: von Willebrand factors (vWFs), hemostatic factors, are produced as large multimers and are shear stress-dependently cleaved to become the appropriate size. A reduction in vWF large multimers develops in various conditions including the use of extracorporeal life support, which can cause excessive-high shear stress in the blood flow and result in hemostatic disorders. The objective of this prospective study was to investigate the impact of venovenous extracorporeal membrane oxygenation (VV ECMO) use on the status of vWF large multimers and hemostatic disorders during single lung transplantation (SLT).
View Article and Find Full Text PDFBackground Central line-associated bloodstream infection (CLABSI) is among the most common bloodstream infections in the university hospital and intensive care unit settings. This study evaluated the routine blood test findings and microbe profiles of bloodstream infection (BSI) by the presence and types of central vein (CV) access devices (CVADs). Methods A total of 878 inpatients at a university hospital who were clinically suspected for BSI and underwent blood culture (BC) testing between April 2020 and September 2020 were enrolled.
View Article and Find Full Text PDFRapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion-Insertion Mutation in S-Protein Gene.
Viruses
May 2023
Rapid molecular testing for severe acute respiratory coronavirus 2 (SARS-CoV-2) variants may contribute to the development of public health measures, particularly in resource-limited areas. Reverse transcription recombinase polymerase amplification using a lateral flow assay (RT-RPA-LF) allows rapid RNA detection without thermal cyclers. In this study, we developed two assays to detect SARS-CoV-2 nucleocapsid (N) gene and Omicron BA.
View Article and Find Full Text PDFStaphylococcus aureus bacteremia results in substantial mortality. Rapid identification and the determination of methicillin susceptibility are crucial for immediate treatment with appropriate antibiotics. In the present study, we aimed to evaluate the basic assay performance of GeneSoC®, a novel rapid quantitative polymerase chain reaction (qPCR) method, for the detection of methicillin-susceptible (MS) or -resistant (MR) S.
View Article and Find Full Text PDFOur facilities acquired ISO 15189 accreditation in April 2011. The lead time was a little less than 2 years. I reported on the lead time and activity after ISO 15189 acquisition.
View Article and Find Full Text PDFBackground: Assessing the drug resistance of leukemic cells is important for treatment of leukemia. We developed a quantitative reverse transcription (RT)-PCR method for multidrug resistance 1 (MDR1) and multidrug resistance-related protein 1 (MRP1) transcripts to evaluate drug resistance, and applied it to clinical samples.
Methods: The cutoffs for copy numbers of MDR1 and MRP1 transcripts were defined based on copy numbers in healthy bone marrow mononuclear cells.