Purpose We aim to determine whether kinematic chain dynamics of the hindfoot and lower leg are involved in the effect of a lateral wedge insole (LWI) on reducing lateral thrust among patients with medial compartment knee osteoarthritis (KOA). Participants and methods Eight patients with knee osteoarthritis were included in the study. Evaluation of the kinematic chain and gait analysis was performed using an inertial measurement unit (IMU).
View Article and Find Full Text PDFThe Dlk1-Dio3 imprinted domain is controlled by an imprinting control region (ICR) called IG-DMR that is hypomethylated on the maternal allele and hypermethylated on the paternal allele. Although several genetic mutation experiments have shown that IG-DMR is essential for imprinting control of the domain, how DNA methylation itself functions has not been elucidated. Here, we performed both gain and loss of DNA methylation experiments targeting IG-DMR by transiently introducing CRISPR/Cas9 based-targeted DNA methylation editing tools along with one guide RNA into mouse ES cells.
View Article and Find Full Text PDF