Spontaneous immortalization of chicken fibroblasts generates stable, high-yield cell lines for serum-free production of cultured meat.

Cellular agriculture could meet growing demand for animal products, but yields are typically low and regulatory bodies restrict genetic modification for cultured meat production. Here we demonstrate the spontaneous immortalization and genetic stability of fibroblasts derived from several chicken breeds. Cell lines were adapted to grow as single-cell suspensions using serum-free culture medium, reaching densities of 108 × 10 cells per ml in continuous culture, corresponding to yields of 36% w/v.

Virtual Online Home-Based Treatment During the COVID-19 Pandemic for Ultra-Orthodox Young Women With Eating Disorders.

With the outbreak of the COVID-19 pandemic, the need arose to maintain treatment continuity for religious Jewish Ultra-Orthodox young women with eating disorders (EDs) previously hospitalized in the ED department at the Ultra-Orthodox "Mayanei Hayeshua" medical center in Israel. This need led to the development of home-based online treatment channels, previously unfamiliar, and unaccepted in this population. The implementation of this model had to take into consideration many of the difficulties inherent in the use of online treatment in Jewish Ultra-Orthodox mental health patients.

Correction to: Extending Data for Urban Health Decision-Making: a Menu of New and Potential Neighborhood-Level Health Determinants Datasets in LMICs.

Readers should note an additional Acknowledgment for this article: Dana Thomson is funded by the Economic and Social Research Council grant number ES/5500161/1.

Extending Data for Urban Health Decision-Making: a Menu of New and Potential Neighborhood-Level Health Determinants Datasets in LMICs.

Area-level indicators of the determinants of health are vital to plan and monitor progress toward targets such as the Sustainable Development Goals (SDGs). Tools such as the Urban Health Equity Assessment and Response Tool (Urban HEART) and UN-Habitat Urban Inequities Surveys identify dozens of area-level health determinant indicators that decision-makers can use to track and attempt to address population health burdens and inequalities. However, questions remain as to how such indicators can be measured in a cost-effective way.

The isolation of novel phage display-derived human recombinant antibodies against CCR5, the major co-receptor of HIV.

Selecting for antibodies against specific cell-surface proteins is a difficult task due to many unrelated proteins that are expressed on the cell surface. Here, we describe a method to screen antibody-presenting phage libraries against native cell-surface proteins. We applied this method to isolate antibodies that selectively recognize CCR5, which is the major co-receptor for HIV entry (consequently, playing a pivotal role in HIV transmission and pathogenesis).

Executive dysfunctions among boys with Attention Deficit Hyperactivity Disorder (ADHD): performance-based test and parents report.

Difficulty in executive functions (EF) is a core symptom of ADHD. Yet, the EF assessments are still in controversy. It is still unclear whether the everyday implementation of EF can be assessed under laboratory conditions.

Participation in leisure activities among boys with attention deficit hyperactivity disorder.

ADHD is a neural developmental disorder expressed in various life settings. Yet, previous studies have focused mainly on children's function in school and academic achievement. The purpose of the present study was, therefore, to examine participation patterns in outside formal school activities among boys with ADHD compared to typical boys.

A method for activity staining of peroxidase and beta-1,3-glucanase isozymes in polyacrylamide electrophoresis gels.

A procedure was developed for activity staining of peroxidase and beta-1,3-glucanase isozymes (a double staining procedure) in the same polyacrylamide gels using 3-amino-9-ethylcarbazol for peroxidase staining and laminarine and 2,3,5-triphenyltetrazolium chloride for glucanase staining. The procedure was used to detect peroxidase and beta-1,3-glucanase in different corn lines using plant leaf extract.

Antifungal activity of volatile fractions of essential oils from four aromatic wild plants in israel.

Essential oils were extracted fromMajorana syriaca, Satureja thymbra, Micromeria fruticosa, andSalvia triloba, and their volatile fractions were tested for their antifungal activity against the soil-borne pathogensFusarium oxysporum andMacrophomina phaseolina and the foliar pathogensBotrytis cinerea andExserohilum turcicum. Results showed a fungistatic effect of 1, 2.5, and 5μl of the various essential oils on fungal mycelium growth.

A method for staining and stabilizing peroxidase activity in polyacrylamide gel electrophoresis.

A procedure was developed for a rapid double staining of peroxidase and other proteins in the same polyacrylamide gels using guaiacol and Coomassie blue. The distinguishable colored bands of peroxidase isozymes and proteins are stable for at least 8 months.

A novel non-centrifugation radioimmunoassay for cannabinoids.

A new method is described for the separation of free and bound fractions in a cannabinoid (3H)-radioimmunoassay without the need for centrifugation. The method employs a novel separation device which allows all manipulations to be performed in a single tube simply, rapidly and safely.