Optimization of ultrasound-assisted extraction based on response surface methodology using HPLC-DAD for the analysis of red clover ( L.) isoflavones and its anti-inflammatory activities on LPS-induced 3D4/2 cell.

Introduction: L. has anti-inflammatory, antioxidant, cardiovascular disease prevention, and estrogen-like effects. The existing method for the assay of effective components is commonly based on a spectrophotometer, which could not meet the requirement of quality control.

Analysis of authorized coccidiostats in chicken feces and environmental water by ultra-performance liquid chromatography-tandem mass spectrometry based on dispersive solid-phase extraction and lyophilization.

A simple, sensitive, and efficient method based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the determination of 8 coccidiostats in chicken feces and environmental water (including sewage, pond water, and lake water) surrounding the farm. Target analytes in chicken feces were extracted with 2% acetic acid in acetonitrile solution, followed by a dispersive solid-phase extraction (DSPE) cleanup step using the mixture of PSA and C adsorbents. Environmental water samples were pretreated using a lyophilization approach.

The pharmacokinetic and residue depletion study of eugenol in carp ().

Introduction: The pharmacokinetic profile and residue depletion of eugenol in carp () tissues and plasma were performed by a convenient and reliable high-performance liquid chromatography (HPLC) method.

Methods: The eugenol in carp tissues and plasma was extracted with a mixed solution of acetonitrile and methanol. N-hexane was used to remove lipid impurities.

Response Surface Optimization of Dispersive Solid-Phase Extraction Combined with HPLC for the Rapid Analysis of Multiple Coccidiostats in Feed.

Since antimicrobials were banned as feed additives, coccidiostats with favorable anticoccidial action and growth promotion have been widely used in the breeding industry. The monitoring of coccidiostats in feed is necessary, while the current methods based on mass-spectrometer analysis have limited applicability and matrix effects could interfere with the results. Accordingly, in the present paper, a rapid analytical strategy for the simultaneous determination of six synthetic coccidiostats in feed using high-performance liquid chromatography coupled with diode-array detection was developed.

Influence of cadmium and microplastics on physiological responses, ultrastructure and rhizosphere microbial community of duckweed.

The combined contamination of heavy metals and microplastics is widespread in freshwater environments. However, there are few researches on their combined effects on aquatic plants. In this study, the effects of single and combined stress of 0.

Ferrate (VI) Oxidation Is an Effective and Safe Way to Degrade Residual Colistin - a Last Resort Antibiotic - in Wastewater.

The rise of novel mobile resistance genes seriously threatens the use of colistin as a last resort antibiotic for treatment of multidrug-resistant Gram-negative bacterial infections in humans. Large quantities of colistin are released annually into the environment through animal feces. This leads to environmental toxicity and promotes horizontal transmission of the gene in aqueous environments.

Research on the Mechanisms of Plant Enrichment and Detoxification of Cadmium.

The heavy metal cadmium (Cd), as one of the major environmentally toxic pollutants, has serious impacts on the growth, development, and physiological functions of plants and animals, leading to deterioration of environmental quality and threats to human health. Research on how plants absorb and transport Cd, as well as its enrichment and detoxification mechanisms, is of great significance to the development of phytoremediation technologies for ecological and environmental management. This article summarises the research progress on the enrichment of heavy metal cadmium in plants in recent years, including the uptake, transport, and accumulation of Cd in plants.

The effect of chelating agents on iron plaques and arsenic accumulation in duckweed (Lemna minor).

Iron plaques have been found to limit the phytoremediation efficiency by reducing iron solubility, while chelating agents can increase the bioavailability of iron from Fe plaques to numerous terrestrial plants. However, the effects of chelating agents on Fe plaques along the As accumulation in aquatic plants remain unknown. In this study, the effects of five chelating agents (EDTA, DTPA, NTA, GLDA, and CA) on the As (As(III) or As(V)), phosphate, and iron uptake by iron plaques and duckweed (Lemna minor) were examined.

Research Progress of a Potential Bioreactor: Duckweed.

Recently, plant bioreactors have flourished into an exciting area of synthetic biology because of their product safety, inexpensive production cost, and easy scale-up. Duckweed is the smallest and fastest-growing aquatic plant, and has advantages including simple processing and the ability to grow high biomass in smaller areas. Therefore, duckweed could be used as a new potential bioreactor for biological products such as vaccines, antibodies, pharmaceutical proteins, and industrial enzymes.

Joint effects of naphthalene and microcystin-LR on physiological responses and toxin bioaccumulation of Landoltia punctata.

The co-contamination of naphthalene (NAP) and microcystin-LR (MC-LR) commonly occurs in eutrophic waters. However, the joint effects of NAP and MC-LR on plants in aquatic environments remain unknown. Landoltia punctata is characterized by high starch yields and high biomass in polluted waters and has been proven to be a bioenergy crop and phytoremediation plant.

miRNA-301b-3p accelerates migration and invasion of high-grade ovarian serous tumor via targeting CPEB3/EGFR axis.

High-grade ovarian serous carcinoma (HGS-OvCa), a type of ovarian cancer with poor prognosis due to distant metastasis, is urgently in need of new therapeutic targets. microRNAs (miRNAs), a class of small noncoding RNAs, perform significant roles in tumor progression. Mounting evidence has revealed the aberrant expression of miRNA in various cancers, one of which is HGS-OvCa.

Rapid screening for Klinefelter syndrome with a simple high-resolution melting assay: a multicenter study.

Klinefelter syndrome (KS) is the set of symptoms that result from the presence of an extra X chromosome in males. Postnatal population-based KS screening will enable timely diagnosis of this common chromosomal disease, providing the opportunity for early intervention and therapy at the time point when they are most effective and may prevent later symptoms or complications. Therefore, through this study, we introduced a simple high-resolution melting (HRM) assay for KS screening and evaluated its clinical sensitivity and specificity in three medical centers using 1373 clinical blood samples.

[Conventional and molecular cytogenetic analyses of a derivative X chromosome in amniocentesis].

Objective: To analyze the aberrant der(X) chromosome using conventional and molecular cytogenetic approaches in a fetus of second trimester and to discuss its clinical effect.

Methods: Conventional cytogenetic procedures (GTG and CBG banding) were performed on cultured amniotic fluid cells. Three-color fluorescence in situ hybridization (FISH) consisting of X chromosome enumeration probes(CEPX), CEPY and Tel Xp/Yp was further performed to study the aberrant der(X) chromosome.

N-[4-(β-d-Allopyranos-yloxy)benzyl-idene]methyl-amine.

The title compound, C(14)H(19)NO(6), was synthesized by the condensation reaction between hecilid (4-formyl-phenl-β-d-allopyran-oside) and methyl-amine in methanol. In the crystal structure, the pyran ring adopts a chair conformation and adjacent mol-ecules are linked by inter-molecular O-H⋯O and O-H⋯N hydrogen bonds, forming a three-dimensional network.