Colorimetric Chemosensor and Turn on Fluorescence Probe for pH Monitoring Based on Xanthene Dye Derivatives and its Bioimaging of Living Escherichia coli Bacteria.

A new turn on fluorescence probe based on 3',6'-dihydroxy-6-methyl-2-((pyridin-2-ylmethylene)amino)-4-(p-tolyl)spiro[benzo[f]isoindole-1,9'-xanthen]-3(2H)-one (BFFPH) derived from benzo[f]fluorescein was prepared. Full characterization of the prepared probe using spectroscopic analysis was described such as IR, NMR and MS spectra. The sensitivity of BFFPH for monitoring of pH change in alkaline medium was studied.

Interspecies comparison of probiotics isolated from different animals.

Aim: The aim of the current study was to isolate and identify naturally occurring probiotic species in different animals with the different environmental background including fish, and farm animals to investigate interspecies differences in probiotics on the species level.

Materials And Methods: A total of 44 fecal and milk samples were collected under aseptic conditions from cattle, buffalo, camel, sheep, goats, and fish. The samples were cultured, and the isolated strains were confirmed biochemically and molecularly using 16S rRNA multiplex polymerase chain reaction (PCR) analysis following DNA extraction from the bacterial isolates.

Serotyping, antibiotic susceptibility, and virulence genes screening of isolates obtained from diarrheic buffalo calves in Egyptian farms.

Aim: In Egypt as in many other countries, river water buffalo () is considered an important source of high-quality milk and meat supply. The objective of this study was to investigate serotypes, virulence genes, and antibiotic resistance determinants profiles of isolated from buffalo at some places in Egypt; noticibly, this issue was not discussed in the country yet.

Materials And Methods: A number of 58 rectal samples were collected from diarrheic buffalo calves in different regions in Egypt, and bacteriological investigated for existence.

MIC and MBC of Honey and Gold Nanoparticles against methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) coagulase-positive isolated from contagious bovine clinical mastitis.

is one of the major causative agents of the bovine clinical mastitis. This study aimed to isolate and identify from cases of bovine clinical mastitis followed by phenotypic detection of MRSA and VRSA. The genotypic detection of MRSA was done through PCR detection of the resistance gene.