Fyn-induced phosphorylation of beta-adducin at tyrosine 489 and its role in their subcellular localization.

Fyn is a Src-family tyrosine kinase involved in neuronal development, transmission, and plasticity in mammalian central nervous system. We have previously reported that Fyn binds to a cytoskeletal protein, beta-adducin, in a phosphorylation-dependent manner. In the present report, we show that Fyn phosphorylates beta-adducin at tyrosine 489 located in its C-terminal tail domain.

Paclitaxel/carboplatin versus cyclophosphamide/adriamycin/cisplatin as postoperative adjuvant chemotherapy for advanced endometrial adenocarcinoma.

Aim: There is no standard chemotherapy regimen for patients with advanced endometrial adenocarcinoma. In our hospital, a cyclophosphamide/adriamycin/cisplatin (CAP) regimen was commonly used as adjuvant chemotherapy. However, since October 1999 a paclitaxel/carboplatin regimen has been substituted for CAP.

[Introduction of rapid HIV tests to a public health center in Japan and evaluation of its effects].

Objective: As an attempt to make HIV testing more accessible to many people, rapid HIV tests were introduced to a public health center. We evaluated logistic issues and effects of the introduction.

Methods: In January 2003, the Tochigi Prefectural Kennan Public Health and Welfare Center introduced rapid HIV tests simultaneously with conventional HIV tests.

Head-head coordination is required for the processive motion of cytoplasmic dynein, an AAA+ molecular motor.

Cytoplasmic dynein is an AAA(+)-type molecular motor whose major components are two identical heavy chains containing six AAA(+) modules in tandem. It moves along a single microtubule in multiple steps which are accompanied with multiple ATP hydrolysis. This processive sliding is crucial for cargo transports in vivo.

Medium-term prognosis of young Japanese adults having acute myocardial infarction.

Background: Data on the medium- and long-term prognosis of young Japanese patients with acute myocardial infarction (AMI) are still lacking.

Methods And Results: In the present study, 1,458 AMI patients were enrolled in the AMI-Kyoto Multi-Center Risk Study between January 2000 and December 2003. Of these, clinical characteristics and medium-term prognosis were retrospectively compared in 21 young patients < 40 years (young group), and 190 non-young patients 60-70 years old (non-young group) who could be followed after hospital discharge.

Gyroscope-like molecules consisting of PdX2/PtX2 rotators encased in three-spoke stators: synthesis via alkene metathesis, and facile substitution and demetalation.

Reactions of trans-MCl2(P((CH2)6(CH=CH2)3)2 (M = a, Pd; b, Pt) and Grubbs' catalyst, followed by hydrogenation (ClRh(PPh3)3), give the title compounds trans-MCl2(P((CH2)14)3P) (2a, 37%; 2b, 43%). These react with LiBr, NaI, and KCN to give the corresponding MBr2, MI2, and M(CN)2 species (58-99%). 13C NMR data show that the MX2 moieties rapidly rotate within the diphosphine cage on the NMR time scale, even at -120 degrees C.

Recurrent severe mitral regurgitation due to left ventricular apical wall motion abnormality caused by coronary vasospastic angina: a case report.

A 76-year-old man developed congestive heart failure due to severe mitral regurgitation after episodes of vasospastic angina. Echocardiography demonstrated left ventricular apical akinesis with ballooning and deformity of the anterior mitral leaflet becoming concave toward the left atrium. The acetylcholine provocation test induced diffuse coronary vasospasm in the distal segments of both right and left coronary arteries and reproduced severe mitral regurgitation.

Role of Src family tyrosine kinases in the down-regulation of epidermal growth factor signaling in PC12 cells.

Src family tyrosine kinases (SFKs) play pivotal roles as molecular switches for various intracellular signaling pathways. SFKs have been implicated in epidermal growth factor (EGF) signaling, although their precise mechanisms of action in this pathway remain elusive. To address this issue, we focused on a membrane microdomain, lipid rafts, where SFKs are enriched.

Acute myocardial infarction in young Japanese adults.

Background: The prevalence and clinical manifestations of acute myocardial infarction (AMI) in young patients in Japan have not been fully investigated.

Methods And Results: In the present study, 1651 AMI patients were enrolled in the AMI-Kyoto Multi-Center Risk Study between January 2000 and June 2004. Of these, the clinical background, risk factors, angiographic findings, acute results of primary percutaneous coronary intervention (PCI) and in-hospital outcomes for 27 young patients <40 (young group), and 338 non-young patients 60 View Article and Find Full Text PDF

Evaluation of transcatheter arterial embolization therapy on hepatocellular carcinomas using contrast-enhanced harmonic power Doppler sonography: comparison with CT, power Doppler sonography, and dynamic MRI.

Purpose: The aim of this study was to assess and compare the sensitivity of power Doppler sonography, contrast-enhanced sonography, plain computed tomography (CT), and dynamic magnetic resonance imaging (MRI) for detecting hepatocellular carcinoma (HCC) nodules incompletely treated with transcatheter arterial embolization (TAE).

Methods: A total of 63 unresectable HCC nodules were examined in this study. The HCCs were treated with TAE.

[Molecular analysis of streptococcal pyrogenic exotoxin genes among group A Streptococcus isolates from Japanese patients with pharyngitis and skin infections].

Group A Streptococci (GAS) from patients with pharyngitis and skin infections were examined for T serotypes, emm types, and streptococcal pyrogenic exotoxin gene types. The results were summarized as follows: 1) T and emm types were determined in 130 GAS isolates obtained between 2000 and 2004. Among 85 throat isolates, predominant T/emm types were T12/emm12 (25%), T4/emm4 (19%), and T1/emm1 (14%).

Retrievable Günther Tulip filter complicated by sepsis and retroperitoneal hemorrhage: successful management by filter retrieval.

We encountered complications of septic shock and retroperitoneal hemorrhage which occurred after implantation of a retrievable Günther Tulip filter. A 79-year-old woman was diagnosed as right femoral vein thrombosis, and a retrievable Günther Tulip filter was deployed prior to a total knee replacement surgery. However, the patient developed septicemia due to Citrobacter freundii, followed by thrombocytopenia and retroperitoneal hemorrhage.

Rapid categorization of pathogenic Escherichia coli by multiplex PCR.

A one-shot multiplex polymerase chain reaction (PCR) was developed for detecting 12 virulence genes of diarrheagenic Escherichia coli. In order to differentiate between the five categories of diarrheagenic E. coli, we selected the target genes: stx1, stx2, and eaeA for enterohemorrhagic E.

Identification of attenuated variants of HIV-1 circulating recombinant form 01_AE that are associated with slow disease progression due to gross genetic alterations in the nef/long terminal repeat sequences.

We identified an unusual case of human immunodeficiency virus type 1 (HIV-1) infection in a patient (GM43) who exhibited a persistently low antibody response and undetectable viral load during a 5-year follow-up period. GM43 harbored HIV-1 circulating recombinant form 01_AE with gross deletions in the nef/long terminal repeat (LTR) region. The sizes of the deletions increased progressively from 84 to >400 bp during the 5-year period.

A case of symptomatic primary HIV infection.

A 30-year-old homosexual Japanese man had fourteen days of fever, malaise, appetite loss, sore throat, and four days of diarrhea and slightly congested eyes before he developed a skin eruption. He presented with measles-like exanthems on his face, trunk, and extremities. Deep red enanthems were seen on his left buccal mucosa opposite the premolar teeth, and whitish enanthems were seen on the buccal and gingival mucosa.

Intra-arterial vasopressin injection for the treatment of massive bleeding from the external carotid artery after craniofacial trauma--technical note.

Vasopressin (0.8-1.0 IU), diluted with saline (10 IU vasopressin per 100 ml saline), was selectively injected into the external carotid artery (ECA) to control massive hemorrhage caused by inaccessible serious craniofacial injuries in two patients.

Heterozygous hemophilia developed during pregnancy.

In rare cases, patients with heterozygous hemophilia show bleeding tendency due to a low factor VIII activity of <20%. Here we describe a case of heterozygous hemophilia that developed during pregnancy. The patient was a 33-year-old Japanese woman whose father and brother both showed elevated nasal bleeding tendencies.

Intraoperative hemodynamic measurements of the vertebral artery and common carotid artery.

The hemodynamics in the vertebrobasilar artery (VBA) system were investigated in patients with vertebrobasilar insufficiency (VBI). Vertebral artery (VA) stump pressure and blood flows in the VA and common carotid artery (CCA) were intraoperatively measured in 45 patients who underwent surgical correction of the first segment of the VA (V1) for angiographic tortuosity, kinking, and/or stenosis manifesting as symptomatic VBI. The effects of changes in the systemic arterial blood pressure (SABP) induced by trimethaphan, phenylephrine, and cervical epidural anesthesia were also investigated.

Cerebral metastasis from a primary adenocarcinoma of the fallopian tube.

Background: Primary adenocarcinoma of the fallopian tube is a rare disease, and cerebral metastasis from this tumor is an extremely rare event.

Case: A 61-year-old female, who had been thought to be in a disease-free state after the initial treatment for stage IIIc fallopian tube adenocarcinoma, presented with severe headache, dizziness, nausea, and vomiting. Brain CT and MRI revealed solitary metastatic lesion in the cerebrum, which was the only site of recurrence.

[Isolated left ventricular noncompaction in the elderly: a case report].

A 83-year-old man with an implanted pacemaker was admitted to our hospital for dyspnea. Echocardiography revealed left ventricular dilation and diffuse hypokinesis mimicking dilated cardiomyopathy. Multiple trabeculations and deep intertrabecular recesses were prominent in the apical and posterior walls.

Characterization and evolution of major histocompatibility complex class II genes in the aye-aye, Daubentonia madagascariensis.

Major histocompatibility complex genes (Mhc-DQB and Mhc-DRB) were sequenced in seven aye-ayes (Daubentonia madagascariecsis), which is an endemic and endangered species in Madagascar. An aye-aye from a north-eastern population showed genetic relatedness to individuals of a north-western population and had a somewhat different repertoire from another north-eastern individual. These observations suggest that the extent of genetic variation in Mhc genes is not excessively small in the aye-aye in spite of recent rapid destruction of their habitat by human activities.

Alkene metatheses in transition metal coordination spheres: dimacrocyclizations that join trans positions of square-planar platinum complexes to give topologically novel diphosphine ligands.

The alkene-containing phosphines PPh((CH2)(n)CH=CH2)2)2 are prepared from PPhH(2), n-BuLi, and the corresponding bromoalkenes (1:2:2), and combined with the platinum tetrahydrothiophene complex [Pt(mu-Cl)(C(6)F(5))(S(CH2CH2(-))2)]2 to give the square-planar adducts trans-(Cl)(C(6)F(5))Pt(PPh((CH2)(n)CH=CH2)2)2 (11, 93-73%; n=a, 2; b, 3; c, 4; d, 5; e, 6; f, 8). Ring-closing metatheses with Grubbs' catalyst (2) are studied. With, two isomers of trans-(Cl)(C6F5)[formula: see text](14)Ph)(15e) are isolated after hydrogenation.