Respiratory vaccination.

Vaccinations are among the most cost-effective preventive health strategies to reduce healthcare costs and prevent morbidities. Every year, many adults and elderly encounter hospitalization because of infectious respiratory diseases. Among these, viral and bacterial pneumonia, tuberculosis, diphtheria and pertussis infections are some of the diseases that can be prevented and managed with fewer complications, with adequate preventive immunization.

Antimicrobial prescribing patterns of surgical speciality in a tertiary care hospital in India: Role of persuasive intervention for changing antibiotic prescription behaviour.

Background: Inappropriate use of antibiotics globally has been linked to increase in antibiotic resistance.

Objectives: This interventional study assessed the impact of antibiotic prescription feedback and focus group discussions (FGD) on hospital-based prescribers before and after the FGD.

Study Design: The present study was performed at a tertiary care centre in New Delhi, wherein 45 units from surgical specialities were included for FGD.

Threshold of galactomannan antigenemia positivity for early diagnosis of invasive aspergillosis in neutropenic children.

Purpose: Invasive aspergillosis (IA) is an important cause of morbidity and mortality in immunocompromised patients. Pediatric data on the accuracy and optimal cutoff of galactomannan antigen detection to diagnose IA is sparse and controversial. We evaluated the utility and optimal serum galactomannan assay (GA) cutoff in children.

Small-molecule proteostasis regulators for protein conformational diseases.

Protein homeostasis (proteostasis) is essential for cellular and organismal health. Stress, aging and the chronic expression of misfolded proteins, however, challenge the proteostasis machinery and the vitality of the cell. Enhanced expression of molecular chaperones, regulated by heat shock transcription factor-1 (HSF-1), has been shown to restore proteostasis in a variety of conformational disease models, suggesting this mechanism as a promising therapeutic approach.

On-line characterization of physiological state in poly(beta-hydroxybutyrate) production by Wautersia eutropha.

Culture fluorescence measurement technique has the potential for on-line characterization of metabolic status of fermentation processes. Many fluorophores present inside the living cells such as NADH + H+, tryptophan, pyridoxine, and riboflavin fluoresce at specific excitation and emission wavelength combinations. Since these key intracellular metabolites are involved in cell growth and metabolism, their concentration change at any time inside the cell could reflect the changes in cell metabolic activity.

Production of poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) having a high hydroxyvalerate content with valeric acid feeding.

The capability of different organic acids to produce a derivative of PHB [poly(3-hydroxybutyric-co-3-hydroxyvaleric acid), P(3HB-co-3HV)] was examined in shake flask cultivations. Propionic and valeric acids demonstrated the potential to produce P(3HB-co-3HV) under nitrogen limiting conditions at 30 degrees C. The addition time and the initial concentration of valeric acid needed for a high cellular HV content were identified by extensive experimentation.

Repeated batch cultivation of Ralstonia eutropha for Poly (beta-hydroxybutyrate) production.

Batch cultivation of Ralstonia eutropha NRRL B14690 attained 21 g biomass l(-1) and 9.4 g poly(beta-hydroxybutyrate) l(-1) (0.45 g PHB g(-1 )dry wt(-1)) in 60 h.

A simple structured mathematical model for biopolymer (PHB) production.

Economic production technology for a biodegradable polymer (poly-beta-hydroxybutyrate, PHB) is urgently required to replace conventional polymers, which have an inherent disadvantage of staying in the environment forever. Various approaches have been applied for improving the productivity and reducing the production cost, which are considered to be the two major problems associated with industrial production of PHB. One of the engineering approaches to improve PHB productivity could be to design and implement model-based fed-batch cultivations to provide desirable nutrient availability.