Magneto-conducting multifunctional Janus microbots for intracellular delivery of biomolecules.

Although several advances have been made in the field of medicine during the last few decades, yet targeted delivery of biomolecules is still a significant challenge. Thus, the present study illustrates the fabrication of dual nature magneto-conducting Fe O -SU8 derived carbon-based Janus microbots that could deliver biomolecules efficiently inside cells. These microsystems possess dual properties, that is, the half part is magneto-conducting, and another half is only conducting for sufficing the therapeutic payloads efficiently under electromagnetic stimulations.

Magnetic nanofibers based bandage for skin cancer treatment: a non-invasive hyperthermia therapy.

Background: The treatment of non-melanoma skin cancer and deadliest malignant melanoma skin cancer are the fifth and ninth most expensive treatments in Medicare, respectively. Moreover, the recurrence of cancer after currently available therapies, that is, surgery or radiotherapy, reduces the patient's life expectancy.

Aims: In view of this, we fabricated magnetic nanofibrous mat-based bandage to treat skin cancer non-invasively using an external alternating current (AC) magnetic field induced hyperthermia.

Remarkably selective biocompatible turn-on fluorescent probe for detection of Fe in human blood samples and cells.

The robust nature of a biocompatible fluorescent probe is demonstrated, by its detection of Fe even after repeated rounds of quenching (reversibility) by acetate in real human blood samples and cells . Significantly trace levels of Fe ions up to 8.2 nM could be detected, remaining unaffected by the existence of various other metal ions.

A Composite of Hyaluronic Acid-Modified Graphene Oxide and Iron Oxide Nanoparticles for Targeted Drug Delivery and Magnetothermal Therapy.

Graphene oxide (GO) nanoparticles have been developed for a variety of biomedical applications as a number of different therapeutic modalities may be added onto them. Here, we report the development and testing of such a multifunctional GO nanoparticle platform that contains a grafted cell-targeting functionality, active pharmaceutical ingredients, and particulates that enable the use of magnetothermal therapy. Specifically, we demonstrate the ability to covalently attach hyaluronic acid (HA) onto GO, and the resultant nanoparticulates (GO-HA) exhibited low inherent toxicity toward two different breast cancer cell lines, BT-474 and MDA-MB-231.

Chitosan-Glycerol Gel as Barrier Formulation for Metal Allergy.

Metal-induced allergic contact dermatitis, particularly nickel, affects over 10% of the general population. Herein, chitosan-glycerol gel as protective barrier formulation was synthesized by neutralization reaction with an aim to reduce metal-ion diffusion into the skin to prevent allergy. Active functional groups in chitosan-glycerol gel were able to capture allergenic metal ions present in artificial sweat solution.

Synthesis of Graphene Oxide-FeO Based Nanocomposites Using the Mechanochemical Method and in Vitro Magnetic Hyperthermia.

The study presented in this work consists of two parts: The first part is the synthesis of Graphene oxide-FeO nanocomposites by a mechanochemical method which, is a mechanical process that is likely to yield extremely heterogeneous particles. The second part includes a study on the efficacy of these Graphene oxide-FeO nanocomposites to kill cancerous cells. Iron powder, ball milled along with graphene oxide in a toluene medium, underwent a controlled oxidation process.

Magnetic hyperthermia adjunctive therapy for fungi: studies against .

The poor penetration of anti-fungal agents into the cornea through the intact epithelium layer makes it difficult to treat acute fungal corneal infections. Herein, we developed Amphotret (amphotericin B) antifungal drug contained polycaprolactone-FeO (PCL-FO) magnetic nanofibers (MNFs) using the electrospinning technique. These MNFs generate heat in the presence of AC magnetic field (AMF) and release drug upon heating.

Single coating of zinc ferrite renders magnetic nanomotors therapeutic and stable against agglomeration.

Magnetic nanomotors with integrated theranostic capabilities can revolutionize biomedicine of the future. Typically, these nanomotors contain ferromagnetic materials, such that small magnetic fields can be used to maneuver and localize them in fluidic or gel-like environments. Motors with large permanent magnetic moments tend to agglomerate, which limits the scalability of this otherwise promising technology.

The combined effect of thermal and chemotherapy on HeLa cells using magnetically actuated smart textured fibrous system.

Thermal therapy combined with chemotherapy is one of the advanced and efficient methods to eradicate cancer. In this work, we fabricated magnetically actuated smart textured (MAST) fibrous systems and studied their candidacy for cancer treatment. The polycaprolactone-Fe O based MAST fibers were fabricated using electrospinning technique.

Study of smart antibacterial PCL-xFe O thin films using mouse NIH-3T3 fibroblast cells in vitro.

Surface energy plays a major role in prokaryotic and eukaryotic cell interactions with biomedical devices. In the present study, poly(ε-caprolactone)-xFe O nanoparticles (PCL-xFO NPs; x = 0, 10, 20, 30, 40, 60 wt% FO concentration in PCL) composite thin films were developed for skin tissue regeneration. The surface properties in terms of roughness, surface energy, wettability of the thin films were altered with the incorporation of Fe O NPs.

Vertical electric field stimulated neural cell functionality on porous amorphous carbon electrodes.

We demonstrate the efficacy of amorphous macroporous carbon substrates as electrodes to support neuronal cell proliferation and differentiation in electric field mediated culture conditions. The electric field was applied perpendicular to carbon substrate electrode, while growing mouse neuroblastoma (N2a) cells in vitro. The placement of the second electrode outside of the cell culture medium allows the investigation of cell response to electric field without the concurrent complexities of submerged electrodes such as potentially toxic electrode reactions, electro-kinetic flows and charge transfer (electrical current) in the cell medium.

Intracellular reactive oxidative stress, cell proliferation and apoptosis of Schwann cells on carbon nanofibrous substrates.

Despite considerable research to develop carbon based materials for biomedical applications, the toxicity of carbon remains a major concern. In order to address this issue as well as to investigate the cell fate processes of neural cells from the perspective of neural tissue engineering applications, the in vitro cytocompatibility of polyacrylonitrile (PAN) derived continuous carbon nanofibers and PAN derived carbon thin films were investigated both quantitatively and qualitatively using in vitro biochemical assays followed by extensive flow cytometry analysis. The experimental results of Schwann cell fate, i.

In vitro cytocompatibility assessment of amorphous carbon structures using neuroblastoma and Schwann cells.

The development of scaffolds for neural tissue engineering application requires an understanding of cell adhesion, proliferation, and migration of neuronal cells. Considering the potential application of carbon as scaffold materials and the lack of understanding of compatibility of amorphous carbon with neuronal cells, the carbon-based materials in the forms of carbon films and continuous electrospun carbon nanofibers having average diameter of ~200 nm are being investigated with or without ultraviolet (UV) and oxy-plasma (OP) treatments for cytocompatibility property using mouse Neuroblastoma (N2a) and rat Schwann cells (RT4-D6P2T). The use of Raman spectroscopy in combination with Fourier transform infrared (FTIR) and X-ray diffraction establishes the amorphous nature and surface-bonding characteristics of the studied carbon materials.