A Review of Basic Fibroblast Growth Factor Delivery Strategies and Applications in Regenerative Medicine.

Basic fibroblast growth factor (bFGF) is a significant member of the fibroblast growth factor (FGF) family. The bFGF has a three-dimensional structure comprising 12 reverse parallel β-folds. This structure facilitates tissue wound repair, angiogenesis, bone formation, cartilage repair, and nerve regeneration.

Heterocoupling Two Similar Benzyl Radicals by Dual Photoredox/Cobalt Catalysis.

Transition-metal-regulated radical cross coupling enables the selective bonding of two distinct transient radicals, whereas the catalytic method for sorting two almost identical transient radicals, especially similar benzyl radicals, is still rare. Herein, we show that leveraging dual photoredox/cobalt catalysis can selectively couple two similar benzyl radicals. Using easily accessible methylarenes and phenylacetates (benzyl N-hydroxyphthalimide (NHPI) esters) as benzyl radical sources, a range of unsymmetrical 1,2-diarylethane classes via the 1°-1°, 1°-2°, 1°-3°, 2°-2°, 2°-3° and 3°-3° couplings were obtained with broad functional group tolerance.

Polyurethane Elastomers Strengthened by Pseudo[1]rotaxanes Based on Pillararenes.

As a unique property of the interlocked structures, rotaxane allows for intramolecular motions between its wheel and axle components. Introduction of rotaxanes into polymers can endow them with distinctive macroscopic features and outstanding mechanical properties. Here, we prepare a copillar[5]arene with a hydroxyl and an amino-group on each end, which can spontaneously form a pseudo[1]rotaxane through intramolecular hydrogen bonds.

Multiple Cross Displacement Amplification Combined with Real-Time Fluorescence Monitoring for Efficient, Specific, and Sensitive Detection.

Invasive meningococcal disease, caused by (), is a critical global health issue, necessitating swift and precise diagnostics for effective management and control. Here, we introduce a novel diagnostic assay, NM-RT-MCDA, that combines multiple cross displacement amplification (MCDA) with real-time fluorescence detection, targeting a specific gene region in the genome. The assay utilizes a primer set designed for high specificity and incorporates a fluorophore-quencher pair with a restriction endonuclease site for real-time monitoring.

Clinical features and two-year outcomes in systemic lupus erythematosus patients with heart failure and reduced, mid-range and preserved ejection fractions.

Objective: We retrospectively analyzed the clinical features and prognosis of SLE patients with HF.

Methods: Patients with SLE and HF who were hospitalized in Jinling Hospital from January 2013 to May 2021 and followed up for 2 years after discharge were included. Risk factors for death and ESKD were analyzed.

Ultra-rapid and sensitive detection of African swine fever virus using multiple cross displacement amplification combined with nanoparticle-based lateral flow biosensor.

African swine fever (ASF) is a devastating disease that can kill almost all infected pigs, causing great damage to the pig industry and destabilizing the global economy. Here, we developed a specific assay that combined multiple cross-displacement amplification (MCDA) with a nanoparticle-based lateral flow biosensor (LFB) for early and rapid identification of the African swine fever virus (ASFV-MCDA-LFB). We first designed a set of MCDA primers to recognize 10 different regions of the target ASFV gene.

A viral protein activates the MAPK pathway to promote viral infection by downregulating callose deposition in plants.

Mitogen-activated protein kinase (MAPK) cascades are evolutionarily conserved in both plants and animals and play critical roles in activating innate immunity to defend against various pathogens. However, the role of MAPK cascades in positively regulating or enhancing viral infections in plants is unclear. In this study, we investigate the involvement of MAPK cascades in infection by the positive-strand RNA virus tomato chlorosis virus (ToCV).

Unraveling the resistance mechanism of anammox granular sludge to iron nanoparticles.

Iron nanoparticles (FeNPs) generated from industrial activities could end up into the sewer system, and potentially affect wastewater treatment processes. The impact of FeNPs on anammox process is getting increasing attention. However, the resistance mechanism of anammox granular sludge (AnGS) to FeNPs has not been fully elucidated.

Dual ligand-enabled iron and halogen-containing carboxylate-based photocatalysis for chloro/fluoro-polyhaloalkylation of alkenes.

Herein, we demonstrate a practical dual ligand-enabled iron photocatalysis paradigm-converting all kinds of halogen-containing carboxylates (C X COO, X: F, Cl, Br) into C X radicals for the valuable chloro/fluoro-polyhaloalkylation of non-activated alkenes with easily available trichloroacetonitrile/Selectfluor as the electrophilic halogenation reagent. The modular assembly of the effective iron and C X COO-based light-harvesting species using the two ligands-OMe/CF-substituted bipyridine and acetonitrile/trichloroacetonitrile is evidenced by detailed mechanistic studies. The late-stage modification, low loading amount of iron (TON: 257) and feasible gram-scale synthesis show the utility of this protocol.

Poultry Nutrition: Achievement, Challenge, and Strategy.

Poultry, a vital economic animal, provide a high-quality protein source for human nutrition. Over the past decade, the poultry industry has witnessed substantial achievements in breeding, precision feeding, and welfare farming. However, there are still many challenges restricting the sustainable development of the poultry industry.

Diabetic adverse events associated with three commonly used statins: a disproportionality analysis based on the FDA adverse event reporting system database.

Background: The increasing prevalence of statin use for cardiovascular disease management has raised concerns regarding their safety profile, particularly regarding the potential risk of diabetes. Our study aims to analyze diabetic adverse event reports related to statins using a large pharmacovigilance database to provide timely insights into this significant issue.

Methods: We analyzed data from the FDA Adverse Event Reporting System (FAERS) database from 2004 to 2023.

Research on the Behavior Recognition of Beef Cattle Based on the Improved Lightweight CBR-YOLO Model Based on YOLOv8 in Multi-Scene Weather.

In modern animal husbandry, intelligent digital farming has become the key to improve production efficiency. This paper introduces a model based on improved YOLOv8, Cattle Behavior Recognition-YOLO (CBR-YOLO), which aims to accurately identify the behavior of cattle. We not only generate a variety of weather conditions, but also introduce multi-target detection technology to achieve comprehensive monitoring of cattle and their status.

Bifunctional iron-catalyzed alkyne Z-selective hydroalkylation and tandem Z-E inversion via radical molding and flipping.

The challenging synthesis of thermodynamic-unfavored cis-olefins through catalytic cross-coupling reactions requires the synergistic interaction of substrate-activating units and configuration-regulating catalysts. Successfully hitting these two birds with one stone, we herein develop a convenient photoredox access to Z-alkenes from alkynes and light alkanes with a bifunctional iron-catalyzed system possessing both C(sp)-H activation and configuration-controlling abilities. The protocol exhibits 100% atom utilization, mild conditions, a broad substrate scope, and compatibility with multitudinous functional groups.

Monitoring the Biochemical Activity of Single Anammox Granules with Microbarometers.

Granule-based anaerobic ammonium oxidation (Anammox) is a promising biotechnology for wastewater treatments with extraordinary performance in nitrogen removal. However, traditional analytical methods often delivered an average activity of a bulk sample consisting of millions and even billions of Anammox granules with distinct sizes and components. Here, we developed a novel technique to monitor the biochemical activity of individual Anammox granules in real-time by recording the production rate of nitrogen gas with a microbarometer in a sealed chamber containing only one granule.

Multi-site DNA methylation alterations of peripheral blood mononuclear cells serve as novel biomarkers for the diagnosis of AIS/stage I lung adenocarcinoma: A multi-center cohort study.

Background: Early diagnosis remains an obstacle for improving the outcome of lung adenocarcinoma (LUAD). DNA methylation changes in peripheral blood mononuclear cells (PBMCs) could reflect immune response to tumorigenesis, providing the theoretical basis for early cancer diagnosis based on immune cell profiling.

Methods: This multi-center study evaluated the DNA methylation patterns based on PBMCs samples from 1115 individuals at nine medical centers.

Epidemiological characteristics, diagnosis and treatment effect of rifampicin-resistant pulmonary tuberculosis (RR-PTB) in Guizhou Province.

Background: Rifampicin-resistant pulmonary tuberculosis (RR-PTB) presents a significant threat to global public health security. China bears a substantial burden of RR-PTB cases globally, with Guizhou Province experiencing particularly alarming trends, marked by a continual increase in patient numbers. Understanding the population characteristics and treatment modalities for RR-PTB is crucial for mitigating morbidity and mortality associated with this disease.

Liposomal nano-encapsulation of bFGF combined with injectable BSA hydrogel for efficient burn wound healing.

Rapid and scar-free healing of burn wounds is an urgent clinical issue. Basic fibroblast growth factor (bFGF) has been proven to promote the healing of burn wounds by accelerating ECM remodeling and angiogenesis. However, exudates from burn wounds can accelerate bFGF degradation, thereby affecting its bioactivity.

Exploring the relationship between rearing system and carcass traits of Danzhou chicken: a microbial perspective.

This study investigated the effects of free-range (FR) and cage-rearing (CR) systems on intestinal health, carcass traits, and microbial diversity in the Danzhou chicken breed. Two groups of 125 hens in each group, aged 42 wk, were reared under FR and CR systems. At 50 wk, 50 hens from each group were randomly selected for carcass analysis and 10 hens for intestinal morphology and microbiota profiling.

CXCL5 Promotes the Malignant Phenotype of Pancreatic Cancer and Is Associated With Immune Infiltration.

Background: The significance of CXCL5 in pancreatic cancer is unclear, although it has been implicated in the malignant process of many different types of cancer. Research on the impact of CXCL5 on immune cell infiltration and the malignant phenotype of pancreatic cancer is needed. This study aimed to examine the connection between CXCL5 expression and immune cell infiltration and the malignant phenotype of pancreatic cancer.

Impact of patent foramen ovale with left-to-right shunt on atrial fibrillation ablation in young patients.

Objective: The CABANA study shows that atrial fibrillation (AF) paitents younger than 65 years benefit more from the AF radiofrequency catheter ablation (RFCA) procedure. The aim of this study is to investigate the impact of inherent patent foramen ovale (PFO) with a Left-to-Right Shunt on the RFCA procedure in young AF patients.

Methods: Based on the presence or absence of inherent PFO, the AF patients were divided into the PFO groups and the non-PFO group.