The ROCK inhibitor netarsudil in the treatment of corneal endothelial decompensation caused by corneal endotheliitis: A case report and literature review.

Proper hydration and the clarity of the cornea are maintained through the crucial function of the corneal endothelium. Inflammation of the corneal endothelium, known as endotheliitis, can disrupt endothelial function, resulting in alterations to vision. Corneal endotheliitis is characterised by corneal oedema, the presence of keratic precipitates, inflammation within the anterior chamber, and occasionally, limbal injection, neovascularisation, and the concurrent or overlapping presence of uveitis.

Corneal subbasal nerve plexus reinnervation and stromal cell morphology with different cap thicknesses in small incision lenticule extraction.

Purpose: The corneal cap thickness is a vital parameter designed in small incision lenticule extraction (SMILE). The purpose was to investigate the changes in corneal subbasal nerve plexus (SNP) and stromal cells with different cap thicknesses and evaluate the optimized design for the surgery.

Methods: In this prospective, comparative, non-randomized study, a total of 108 eyes of 54 patients who underwent SMILE were allocated into three groups with different corneal cap thicknesses (110 μm, 120 μm or 130 μm group).

A Mechanical Evaluation of a Robot-Assisted Cutting Cornea Based on Force Response.

The aim of this paper is to propose laws of trephine operation based on a robot-assisted cutting cornea in order to obtain better microsurgical effects for keratoplasty. Using a trephine robot integrated with a microforce sensor and a handheld trephine manipulator, robotic and manual experiments were performed, with porcine corneas as the test subjects. The effect of trephine operational parameters on the results reflected by the biomechanical response is discussed, and the parameters include linear velocity, rotating angle, and angular velocity.

Dry eye disease due to meibomian gland dysfunction treated with Pinggan Yuyin Qingre formula: a stratified randomized controlled trial.

Objective: To observe the clinical efficacy on improving the quality of meibum in patients suffer from dry eye disease (DED) due to meibomian gland dysfunction (MGD) with hyperactivity ofdue todeficiency pattern after being treated with Pinggan Yuyin Qingre formula (, PGYYQR).

Methods: Totally 120 patients who met the inclusion criteria were included and stratified into three levels according to the level of MGD (1-3), and patients in each level was randomly allocated into the treatment group and control group according to a 1∶1 ratio. Both groups were treated with sodium hyaluronate eye drops, and the treatment group was also given PGYYQR granules.

Clinical study on the effect of preloaded punctal plug in the treatment of aqueous-deficient dry eye.

Objective: To evaluate the effect of OASIS preloaded punctal plug versus Smart Plug punctal plug Retrospectively registered in the treatment of aqueous-deficient dry eye.

Methods: 47 patients were randomly divided into a control group and an experimental group. The control group was treated with Smart Plug punctal plug treatment, and the experimental group was treated with OASIS preloaded punctal plug treatment.

Clinical outcomes of modified simple limbal epithelial transplantation for limbal stem cell deficiency in Chinese population: a retrospective case series.

Objective: To report the clinical outcomes of a novel surgical technique, namely simple limbal epithelial transplantation (SLET), for the treatment of limbal stem cell deficiency (LSCD).

Methods: Thirteen patients (13 eyes) with LSCD who underwent autologous (10 eyes) or allogeneic (3 eyes) modified SLET between 2018 and 2021 were enrolled in this study. Grades of symblepharon, corneal conjunctivalization, vascularization, opacification, and visual acuity (VA) were evaluated preoperatively and postoperatively.

Three-dimensional biomechanical modeling and simulation of trephine cutting cornea for keratoplasty.

Purpose: Trephination is one of the basic operations of keratoplasty, and the biomechanical mechanism of the operation can be revealed based on three-dimensional modeling and simulation of trephine cutting cornea.

Methods: Based on the analysis of the physical and biomechanical characteristics of corneal trephination, a three-dimensional numerical model of corneal trephination is built, where the cornea can be simplified to two layers structure including stroma and epithelium, and the trephine cuts the cornea under the vertical motion load and the rotational motion load. A three-dimensional failure criterion of corneal material is proposed based on the yield strength theory.

Etiological characteristics of chlamydia trachoma conjunctivitis of Primary Boarding School students in the Qinghai Tibetan area.

The aim of this study was to investigate the etiological characteristics of Chlamydia trachomatis conjunctivitis among resident students at primary schools in the Qinghai Tibetan area in order to understand the distribution of C. trachomatis and other pathogenic microorganisms, to detect the isolation rate of infectious pathogens, and to provide an evidence for further targeted efforts in the prevent of sporadic trachoma efforts. From two primary schools in Qinghai Province, ocular samples from 35 students who were clinically diagnosed as trachoma cases and 60 normal controls were obtained by swabbing their upper eyelids and lower conjunctival sacs.

A review of trachoma history in China: research, prevention, and control.

Trachoma is one of the most widespread blinding eye diseases, which is harmful to human visual health. The efforts to prevent and control trachoma in China can be divided into three periods, i.e.

Dry Eye Syndrome in Patients with Diabetes Mellitus: Prevalence, Etiology, and Clinical Characteristics.

There has been substantial progress in our understanding of the ocular surface system/lacrimal function unit in the past 15 years. Keratoconjunctivitis sicca, more commonly referred to as dry eye syndrome (DES), is the most frequently encountered condition and diabetes mellitus (DM) has been identified as one of the leading causes of DES. Poor glycemic control affects both the anterior and the posterior segments of the eye and increasing prevalence of diabetes-associated DES (DMDES) has been reported in recent years.

Corneal subbasal nerve fiber regeneration in myopic patients after laser in situ keratomileusis.

A total of 26 myopic patients (52 eyes) underwent laser in situ keratomileusis. In vivo confocal microscopy revealed that most of the regenerated corneal subbasal nerve fibers in the corneal flap originated from the stump of corneal subbasal nerve fibers outside the ablation zone and extended towards the center of the cornea in all patients. Meanwhile, new fibers were also found to directly regenerate from deep in the stroma in some cases.

Confocal comparison of corneal nerve regeneration and keratocyte reaction between FS-LASIK, OUP-SBK, and conventional LASIK.

Purpose: We compared the regeneration characteristics of injured corneal subbasal nerve fibers (CSNFs) and keratocyte reaction following LASIK with a femtosecond laser (FS-LASIK), One Use-Plus Sub-Bowman's keratomileusis (OUP-SBK), and M2 90 microkeratome to provide a more suitable treatment strategy.

Methods: A total of 22 eyes that underwent FS-LASIK, 32 eyes that underwent OUP-SBK, and 26 eyes that underwent conventional LASIK were analyzed by confocal microscopy. Morphologic modifications of corneal architecture were evaluated postsurgically at 1, 3, and 6 months, and comparisons were made by ANOVA analysis.

[In vivo confocal microscopy study of corneal endotheliitis].

Objective: To study the cellular morphological characteristics and changes of corneal endotheliitis by corneal microstructure in vivo.

Methods: Forty-eight clinical diagnosed patients of corneal endotheliitis were examined by in vivo confocal microscopy. Confocal images of different layers were collected and observed.

Insertion force in manual and robotic corneal suturing.

Background: Due to differences in corneal grafting microsurgery between manual and robotic suturing, new challenges have arisen in testing the insertion force and torque of corneal tissue acting on suturing needles in order to guarantee successful completion of surgical procedures.

Methods: In order to measure the force during the insertion operation, from the needle entering the cornea through the entry point until the puncturing of the exit point along the circular trajectory, a force measurement system was established, including fresh porcine cornea, a corneal-suturing robot, a circular needle, a micro-forceps manipulator with a force transducer, a computer with a data acquisition board and a medical microscope. The force values in the needle coordinate frames were obtained on the basis of a sensor coordinate frame through D-H coordinate transformation, and an index is proposed here to evaluate the insertion performance.

[Operation of cornea grafting micro-surgery robot system].

Objective: To design the robotic mechanism of the cornea grafting micro-surgery system and evaluate its experimental feasibility and efficacy.

Methods: It was an experimental study. Based on computer assisted cornea grafting surgery (CACGS), the prototype about cornea grafting robot, which consists of six subsystems, was developed.

Distribution of bacterial keratitis and emerging resistance to antibiotics in China from 2001 to 2004.

Objective: To study on the distribution of bacterial keratitis isolates and the resistance to antibiotics in China from 2001 to 2004.

Methods: 1985 specimens from the bacterial keratitis at the Beijing Tong Ren Eye Center were cultured and identified. In vitro susceptibility testing of positive isolates to antibiotics was determined by the Kirby-Bauer disk diffusion method and interpreted according to Clinical and Laboratory Standards Institute.

[Etiological analysis of ocular non-viral microbial isolates in children and their antibiotics susceptibility in vitro].

Objective: To analyze the types, distribution characteristics and in vitro susceptibility of antibiotic agents of ocular non-viral microbial isolates in children.

Methods: Experimental research.785 specimens obtained from children below the age of 14 in Beijing Tongren Hospital during 1999 to 2004 were cultured and identified for bacterium, fungus and Acanthamoeba respectively at Beijing Institute of Ophthalmology.

[Mutant prevention concentrations of antibacterial agents to ocular pathogenic bacteria].

Objective: To establish a method to measure mutant prevention concentration (MPC) in vitro, and to measure MPC of antibacterial agents for ocular bacteria caused keratitis.

Methods: It was an experimental study. Forty strains of ocular bacteria were separated from cornea in Beijing Institute of Ophthalmology, which included 8 strains of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Pseudomonas aeruginosa and Klebsiella pneumoniae respectively.

Arg124Cys mutation of the TGFBI gene in 2 Chinese families with Thiel-Behnke corneal dystrophy.

Objective: To analyze transforming growth factor beta-induced (TGFBI) gene mutations in 2 Chinese families with Thiel-Behnke corneal dystrophy (TBCD).

Methods: Forty-five individuals in 2 Chinese families with TBCD were examined using slitlamp biomicroscopy. Genomic DNA was extracted from peripheral leukocytes of affected and unaffected family members.

[Analysis on susceptibility of ocular bacterial pathogens to four kinds of fluoroquinolone].

Objective: To assess the in vitro susceptibilities of ocular bacterial isolates to Gatifloxacin and other fluoroquinolones which are often used in ophthalmic treatment.

Methods: Non-randomized, non-comparative, retrospective study. Ocular bacterial isolates (n = 619) from Beijing Institute of Ophthalmology were tested for their susceptibilities to gatifloxacin, ciprofloxacin, ofloxacin and levofloxacin in vitro by the Kirby-Bauer disk diffusion method.

[Movement capture of knot-tying manipulation in microsurgeries].

The surgical dexterous suturing and knotting movements are photographed with binocular stereo vision and the movements' locus is captured through identifying the RGB values in a series of key-frames. Then a normal movement path of knot-tying is obtained through curve-fitting and smoothing. And meanwhile, the workspace of knot-tying manipulation in microsurgeries is got.

Clinical and experimental findings in Acanthamoeba keratitis with Heidelberg Retina Tomograph III-RCM.

Purpose: To observe Acanthamoeba cysts and trophozoites in the corneal tissue of patients and rabbits with Acanthamoeba keratitis (AK), using the Heidelberg Retina Tomograph III Rostock-cornea-module (HRTIII-RCM).

Methods: Eight eyes of seven patients with clinical and laboratory diagnosis of AK, presenting to the Beijing Institute of Ophthalmology between July and December 2006, were included in this study. The laboratory diagnosis in all cases was established by a cytological analysis and culture of the corneal scraping.

[Etiological analysis on bacterial endophthalmitis].

Objective: To investigate the spectrum of organisms causing bacterial endophthalmitis and their sensitivities to commonly used ocular antibiotics.

Methods: A retrospective analysis on microbiological records of 728 intraocular specimens of patients with suspected endophthalmitis from January 1999 to December 2006. Antibiotic susceptibility of these isolates was determined for commonly used ocular antibiotics using the Kirby-Bauer disk-diffusion test.

[Experimental study of non-tuberculous mycobacterial keratitis in rabbits].

Objective: To study clinical manifestation and pathology change of different time points of rabbit keratitis caused by non-tuberculous mycobacteria (NTM).

Methods: Forty-eight eyes from 48 rabbits were randomly divided into three groups:under-flap infection group (UFI group), under-flap infection group with corticosteroid (UFIC group) and surface infection group (SI group). The cornea infiltration area of all rabbits was observed.