MicroRNA Profiling of Exosomes Derived from Red Blood Cell Units: Implications in Transfusion-Related Immunomodulation.

Purpose: To elucidate the microRNAs existent in exosomes derived from stored red blood cell (RBC) unit and their potential function.

Materials And Methods: Exosomes were isolated from the supernatant derived from stored RBC units by sequential centrifugation. Isolated exosomes were characterized by TEM (transmission electron microscopy), western blotting, and DLS (dynamic light scattering).

Combination of Fresh Frozen Plasma and Cryosupernatant Plasma for Therapeutic Plasma Exchange in Thrombotic Thrombocytopenic Purpura: A Single Institution Experience.

Purpose: To evaluate the impact of a combination of fresh frozen plasma (FFP) and cryosupernatant plasma (CP) as a replacement fluid in therapeutic plasma exchange (TPE) on early therapeutic response and long-term survival of patients with thrombotic thrombocytopenic purpura (TTP).

Materials And Methods: A total of 44 patients with suspected TTP were screened by Bentley and PLASMIC scores. Twenty-seven patients treated with TPE using the FFP and CP combination as the replacement fluid were enrolled and divided into two groups: 11 patients who received TPE with CP-dominant replacement fluid (FFP/CP<1) and 16 patients who received TPE with FFP-dominant replacement fluid (FFP/CP>1).

[Arginase Level in Apheresis Leukocyte-Reduced Platelets Stored for Different Time].

Objective: To explore the effect of storage time on arginase level and possible source of arginase in apheresis leukocyte-reduced platelets(ALR-Plt).

Methods: The arginase level and myeloperoxidase(MPO) levels in ALR-Plt and control plasma were detected by ELISA. The relationship between arginase level and MPO level in ALR-Plt was analyzed by correlation analysis.

[Arginase Level in Suspended Red Blood Cells Storaged for Different Time].

Objective: To explore the effect of storage time on arginase level, and the possible source of arginase in suspended red blood cells (RBC).

Methods: The arginase and myeloperoxidase (MPO) levels in suspended RBC and control plasma were detected by ELISA. The free hemoglobin level in suspended RBC and control plasma were detected by colorimetric method.

[Impact of Toxoplasma gondii infection on pregnancy outcomes in early pregnant women].

Objective: To explore the impact of Toxoplasma gondii infection on pregnancy outcomes in early pregnancy women.

Methods: Toxoplasma gondii IgM and IgG antibodies in the peripheral blood of 2 993 early pregnant women were detected by using enzyme-linked immunosorbent assay (ELISA). According to the test results, the infected ones were divided into an acute infection group, a previous infection group, and an active infection group, and 200 pregnant women without Toxoplasma infection were randomly chosen as a control group, and the pregnancy outcomes of the four groups were followed up and the results were compared.

[Efficiency of three methods for detecting Toxoplasma IgG antibody].

Objective: To discuss the test efficiency of three methods for detecting Toxoplasma IgG antibody.

Methods: Totally 304 specimens were detected parallelly for Toxoplasma IgG antibody by using the gold marked method, indirect hemagglutination test (IHA), and enzyme-linked immunosorbent assay (ELISA), and the sensitivity, specificity and Youden index of these methods were compared.

Results: The detection sensitivities of gold marked method, IHA, and ELISA for Toxoplasma IgG antibody were 85.

Ischemia postconditioning and mesenchymal stem cells engraftment synergistically attenuate ischemia reperfusion-induced lung injury in rats.

Background: It has been reported that ischemic postconditioning (IPO) or mesenchymal stem cell (MSC) engraftment could protect organs from ischemia/reperfusion (I/R) injury. We investigated the synergetic effects of combined treatment on lung injury induced by I/R.

Methods: Adult Sprague-Dawley rats were randomly assigned to one of the following groups: sham-operated control, I/R, IPO, MSC engraftment, and IPO plus MSC engraftment.