A strategy for improving the mechanical properties of silk fibers through the combination of genetic manipulation and zinc ion crosslinking.

Silk fiber is generally considered an excellent biological material due to its good biocompatibility, morphological plasticity and biodegradability. Previously, the construction of silkworm silk gland bioreactors based on the piggyBac transposon has been optimized. However, the inserted exogenous genes have problems such as position uncertainty, and expression is not strictly controlled.

In situ growth of HKUST-1 on electrospun polyacrylonitrile nanofibers/regenerated cellulose aerogel for efficient methylene blue adsorption.

Dyes, as organic pollutants, are causing increasingly severe environmental problems. Metal-organic frameworks (MOFs) are considered promising dye adsorbents; however, their application is limited due to their powder or solid particle forms and limited reusability. Therefore, this study proposes an innovative approach to develop a novel MOF-based composite aerogel, specifically a HKUST-1/polyacrylonitrile nanofibers/regenerated cellulose (HKUST-1/PANNs/RC) composite aerogel adsorbent, for the adsorption of pollutants in water.

TALEN-mediated homologous-recombination-based fibroin light chain in-fusion expression system in .

Silkworm was the first domesticated insect and has important economic value. It has also become an ideal model organism with applications in genetic and expression studies. In recent years, the use of transgenic strategies has made the silkworm silk gland an attractive bioreactor for the production of recombinant proteins, in particular, -mediated transgenes.

Development of a Coumarin-derived Fluorescent Probe for Detection of HOCl and its Application in Cells and Zebrafish.

We have prepared a simple, universal and efficient coumarin-derived fluorescent probe (XDS1) to detecting HOCl. The experimental findings revealed that the introduction of HOCl produced an obvious quenching effect on the probe with high selectivity and sensitivity. The calculated limit of detection (LOD) was as low as 0.

Bimetallic Organic Gel for Effective Methyl Orange Dye Adsorption.

A bimetallic organic gel (MOG-Fe/Al) was synthesized through the solvothermal method. The gel state of the product obtained under optimized gel formation conditions is sufficient to carry 2 g of weight for a long time. Scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, Brunauer-Emmett-Teller (BET) technique, and X-ray photoelectron spectroscopy (XPS) analysis confirmed the structures and morphologies of the synthesized materials.

Naphthalimide-based fluorescent probe for Hg detection and imaging in living cells and zebrafish.

A simple naphthalimide-based fluorescent probe was designed and synthesized for the determination of mercury ion (Hg ). The probe showed a noticeable fluorescence quenching response for Hg . When added with Hg , the fluorescence intensity of the probe at 560 nm was remarkably decreased with the color changed from yellow to colorless under ultraviolet (UV) light.

High mechanical property silk produced by transgenic silkworms expressing the Dumpy.

Extensive research has been conducted on utilizing transgenic silkworms and their natural spinning apparatus to produce high-performance spider silk fibers. However, research on using non-spider biological proteins to optimize the molecular structure of silk protein and improve the mechanical performance of silk fibers is still relatively scarce. Dumpy, a massive extracellular matrix polypeptide, is essential for preserving the shape and structural integrity of the insect cuticle due to its remarkable tension and elasticity.

In-situ preparation of lignin/FeO magnetic spheres as bifunctional material for the efficient removal of metal ions and methylene blue.

In this paper, magnetic composite of lignin/FeO spheres were synthesized via a straightforward one-step in-situ solvothermal method showing good capacity for adsorbing heavy metal ions and dyes. The physicochemical properties of lignin/FeO spheres are analyzed using a range of techniques such as SEM, XRD, FTIR, VSM, TG, and BET. Lignin/FeO spheres exhibited high adsorption capacities of 100.

Rhodamine-based Fluorescent Probe With Quick Response and High Selectivity for Imaging Labile Ferrous Iron in Living Cells and Zebrafish.

The transition between its various oxidation states of Iron plays a crucial part in various chemical transformation of cells. Misregulation of iron can give rise to the iron-catalyzed reactive oxygen species disorder which have been linked to a variety of diseases, so it is crucial to monitor the labile iron pool in vivo for clinical diagnosis. According to iron autoxidation and hydrogen abstraction reaction, we reported a novel "off-on" fluorescent probe to response to ferrous (Fe) both in solutions and biological systems.

Modeling the 3-dimensional structure of the silkworm's spinning apparatus in silk production.

The silk-spinning process of the silkworms transforms the liquid silk solution to a solid state under mild conditions, making it an attractive model for bioinspiration However, the precise mechanism behind silk expulsion remains largely unknown. Here we selected the silkworms as representative models to investigate the silk-spinning mechanism. We used serial block-face scanning electron microscopy (SBF-SEM) to reconstruct the three-dimensional structures of the spinnerets in silkworms at various stages and with different gene backgrounds.

The ratiometric fluorescent sensor based on the mixture of CdTe quantum dots and graphene quantum dots for quantitative analysis of silver in drinks.

Silver nanoparticles can be used in antibacterial packaging or disinfection. Research has shown that sugary fluid induces the leaching of silver nanoparticles into water, which may be harmful to humans. Single wavelength fluorescence analysis has been used for quantitative analysis of silver nanoparticles but suffers from low specificity and poor anti-interference ability.

An Ethyl-Thioglycolate-Functionalized FeO@ZnS Magnetic Fluorescent Nanoprobe for the Detection of Ag and Its Applications in Real Water Solutions.

Ethyl-thioglycolate-modified FeO@ZnS nanoparticles (FeO@ZnS-SH) were successfully prepared using a simple chemical precipitation method. The introduction of ethyl thioglycolate better regulated the surface distribution of ZnS, which can act as a recognition group and can cause a considerable quenching of the fluorescence intensity of the magnetic fluorescent nanoprobe, FeO@ZnS-SH. Benefiting from stable fluorescence emission, the magnetic fluorescent nanoprobe showed a highly selective fluorescent response to Ag+ in the range of 0-400 μM, with a low detection limit of 0.

One-pot preparation of MnO/GSH/CdTe quantum dots complex for T-weighted MRI/fluorescence detection of HPO.

Magnetic resonance and fluorescence dual mode imaging with both advantages of high sensitivity and high spatial resolution, which could realize real-time, in situ, and dynamic imaging, is very suitable for detecting small molecules. And the synthesis of imaging materials with good imaging performance has become the core content. In this paper, based on biomimetic mineralization technology, GSH has been used as a material to design a one-pot method for the preparation of MnO/GSH/CdTe quantum dots composites (MGQ in short).

Drosophila melanogaster resilin improves the mechanical properties of transgenic silk.

Resilin is a natural protein with high extensibility and resilience that plays a key role in the biological processes of insects, such as flight, bouncing, and vocalization. This study used piggyBac-mediated transgenic technology to stably insert the Drosophila melanogaster resilin gene into the silkworm genome to investigate whether exogenous protein structures improve the mechanical properties of silkworm silk. Molecular detection showed that recombinant resilin was expressed and secreted into silk.

Sex separation by body color via a W-chromosome-linked transgene.

Sex separation processes are important for commercial insect production and sterile insect techniques. Here, we describe the transgenic insertion of a DsRed expression cassette driven by the enhancer HR3 and strong promoter IE1 into the silkworm W chromosome as a dominant visible marker of sex separation. The obtained transgenic lines showed female-specific body color visible to the naked eye at the second- to fifth-instar larval, pupal and adult stages, and their performance traits were comparable to those of a nontransgenic practical silkworm variety.

Synthesis and Application of Ion-Exchange Magnetic Microspheres for Deep Removal of Trace Acetic Acid from DMAC Waste Liquid.

In order to develop a deep method for removing trace acetic acid from industrial solvents, a type of quaternary ammonium-salt-modified magnetic microspheres was developed as a potential nanoadsorbent for low-concentration acetic-acid-enhanced removal from DMAC aqueous solution. The ion-exchange magnetic microspheres (FeO@SiO@N(CH)) have been prepared by a two-step sol-gel method with N-trimethoxysilylpropyl-N, N, N-trimethylammonium chloride as functional monomer, tetraethyl orthosilicate as a cross-linking agent, FeO@SiO as a matrix. The nanocomposite is characterized by SEM, FI-IR, XRD, VSM, and XPS.

Intravenous Administration of Hypertonic Glucose Solution to Prevent Dizziness in Patients Undergoing Gastrointestinal Endoscopy Under General Anesthesia: A Randomized Clinical Trial.

Background And Objective: Dizziness is a common complication of gastrointestinal endoscopy under general anesthesia. Dizziness is primarily caused by a lack of energy and blood volume following fasting and water deprivation. Hypertonic glucose solution (HGS) is an intravenous energy replenishment, that increases blood volume due to its hyperosmotic characteristics and can be directly absorbed from blood circulation.

Controllable Preparation of Superparamagnetic FeO@La(OH) Inorganic Polymer for Rapid Adsorption and Separation of Phosphate.

Superparamagnetic Fe3O4 particles have been synthesized by solvothermal method, and a layer of dense silica sol polymer is coated on the surface prepared by sol-gel technique; then La(OH)3 covered the surface of silica sol polymer in an irregular shape by controlled in situ growth technology. These magnetic materials are characterized by TEM, FT-IR, XRD, SEM, EDS and VSM; the results show that La(OH)3 nanoparticles have successfully modified on Fe3O4 surface. The prepared Fe3O4@La(OH)3 inorganic polymer has been used as adsorbent to remove phosphate efficiently.

The detection of multiple analytes by using visual colorimetric and fluorometric multimodal chemosensor based on the azo dye.

In recent decades, researchers have conducted in-depth studies of the design and synthesis of colorimetric/fluorometric probes and the application of such probes to biological and practical samples. The multifunctional colorimetric and fluorescent azo benzene-based probe (4'-hydroxyl-2,4-diaminoazobenzene, ) was designed to detect Al, Fe, Cu and F¯. Based on the distinct redshift of the absorption band and a significant color change (yellow → purple), was utilized for both naked-eyed and quantitative detection of Al and Fe after formation of the 1:1 complex.

The preparation of MnO/BSA/CdTe quantum dots complex for ratiometric fluorescence/ T-weighted MRI detection of HO.

Hydrogen peroxide, as the second messenger in cells, plays an essential regulatory role in cell proliferation, differentiation and migration. How to effectively identify HO signals, and detect HO in circulating tumor cells, are essential for the early diagnosis of tumors. Herein a fluorescence and T-weighted MR dual-mode imaging material, named MnBQ, has been successfully prepared and characterized.

A novel mitochondria-targetable NIR fluorescent probe for monitoring intracellular hypobromous acid levels.

The development of ultrasensitive in situ detection techniques for monitoring hypobromous acid (HBrO) levels in the biological systems is of great significance to reveal its complex pathological and physiological effects. A simple mitochondria-targetable hydrazine-based near-infrared (NIR) fluorescent probe (Mito-NIR) for detecting HBrO in the mitochondria of live cells is presented in this paper. Probe Mito-NIR displays the ultrafast (< 5 s) response for HBrO.

The Development of a 4-aminonaphthalimide-based Highly Selective Fluorescent Probe for Rapid Detection of HOCl.

Recently, more and more evidence indicated that intracellular HOCl plays a crucial role in the regulation of inflammation and apoptosis, while excessive HOCl has an impact on human health problems. So, the development of methods for sensitive detection of HOCl is very vital to reveal its various physiological and pathological functions. In this paper, we have described a simple fluorescent probe for selective detection of HOCl, whereas for higher concentrations of other biological important substances, the probe almost does not respond.

Mechanism of silk secretion revealed by proteomic analysis of silkworm cocoons with fibroin light chain mutations.

Silkworm is an economically important insect due to its efficient production of silk proteins. Silk itself and the silk trade have enriched human civilization through art and culture and contributed to early globalization in the Silk Road era for nearly two thousand years. Although a large number of studies on silk have been carried out, the mechanism of silk secretion in silkworms has not been thoroughly studied thus far.

Effects of Additional Intraoperative Administration of Sufentanil on Postoperative Pain, Stress and Inflammatory Responses in Patients Undergoing Laparoscopic Myomectomy: A Double-Blind, Randomized, Placebo-Controlled Trial.

Purpose: Although pain after laparoscopic surgery is assumed to be minor, many women still suffer from unexpected postoperative pain. Thus, we aimed to assess whether additional intraoperative administration of sufentanil could help to improve postoperative pain and related agitation, stress, and inflammation response in patients undergoing laparoscopic myomectomy.

Patients And Methods: Forty female patients with uterine myoma scheduled for laparoscopic myomectomy under general anesthesia were randomized to receive sufentanil (group T, n=20) or normal saline (group C, n=20) 1h before the end of the surgery.

Effect of General and Non-general Anesthesia on Postoperative Cognitive Dysfunction.

ABATRACT Controversy exists in the effect of general anesthesia and non-general anesthesia on postoperative cognitive dysfunction (POCD). The authors aimed to perform this systematic review to comprehensively assess the effect of general anesthesia or non-general anesthesia on POCD. Relative studies from the online literature database were retrieved.