Closed-channel culture system for efficient and reproducible differentiation of human pluripotent stem cells into islet cells.

Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system.

A high-energy 160-ps pulse generation by stimulated Brillouin scattering from heavy fluorocarbon liquid at 1064 nm wavelength.

We have achieved a high compression ratio by stimulated Brillouin scattering (SBS) consisting of two long cells. A 13-ns Nd:YAG laser pulse was temporally compressed to about 160-ps phase-conjugated pulse in heavy fluorocarbon FC-40 liquid at a 1064 nm wavelength. The maximum reflectivity of SBS process was over 80 % without an optical damage.