A safe antiparasitic extract from Psoralea corylifolia for Tetrahymeniasis control.

Tetrahymeniasis is a ciliate disease that presents significant economic challenges for the aquaculture industry. Previous research has shown promising control effects on Tetrahymena pyriformis using flavonoids from Psoralea corylifolia (P. corylifolia), but their high cost hinders practical application.

Screening of Antagonistic Bacteria against Three Aquatic Pathogens and Characterization of Lipopeptides in BA09.

Screening for antagonistic bacteria on aquatic pathogens and identification of antagonistic ingredients are essential to reduce the use of chemicals in aquaculture. In this study, strain BA09, subsequently identified as , simultaneously displayed strong antagonistic effects on , , and in the initial screening and rescreening. In addition, the methanol extract of BA09 was subjected to antibacterial activity verification and one-dimensional (1D) reversed-phase liquid chromatography (RPLC) preparation.

Dynamic pathology and pathogen distribution of the yeast Metschnikowia bicuspidata in the Chinese mitten crab (Eriocheir sinensis).

In this study, the "milky disease" model of Eriocheir sinhensis was constructed via intramuscular injection with the pathogenic yeast Metschnikowia bicuspidata. The dynamic pathological changes of E. sinensis after injection were elucidated with two staining methods (haemotoxylin-eosin and alcian blue periodic acid-Schiff) and fluorescence in situ hybridization technology.

Antiparasitic efficacy of flavonoids identified from Psoralea corylifolia against Tetrahymena piriformis in guppy (Poecilia reticulate).

Tetrahymena piriformis belongs to the ciliated protists (ciliates), causing severe economic losses in aquaculture. Chemical drugs currently used usually have toxic side effects, and there is no specific drug against Tetrahymena. Therefore, it is an urgent need to identify new antiparasitic lead compounds.

The complete mitochondrial genome sequence of the (Metschnikoff, 1884), an emerging pathogen of 'milky disease' in Chinese mitten crab.

The outbreak of milky disease of Chinese mitten crab caused by seriously restricted the development of the crab industry. In this study, the mitochondrial genome sequence of was assembled, annotated, and further analyzed. The results indicated that the complete mitochondrial genome of was 75,095 bp, which contained two rRNAs, 23 tRNAs, and 13 protein-coding genes.

First case of Coxiella burnetii infection in Palaemonetes sinensis cultured in Liaoning, China.

The Palaemonetes sinensis aquaculture industry in Panjin City, Liaoning Province, China, experienced heavy losses in October 2018. Morbidity of cultured shrimp reached 50% and was characterized by cloudiness of muscle and the gradual spread of disease within the population. When the infection was mild, histopathological examinations revealed that the muscle cells contained a considerable number of microorganisms.

RNA-Sequencing Analysis of the Spleen and Gill of in Response to Infection.

is commonly subjected to the disease-causing bacterium, . However, the mechanism involved in the immune response of to infection is unclear. We conducted a transcriptomic analysis of the spleen and gill from infected with .

Metschnikowia bicuspidata isolated from milky diseased Eriocheir sinensis: Phenotypic and genetic characterization, antifungal susceptibility and challenge models.

A severe disease occurred in farmed Eriocheir sinensis characterized by milky liquid accumulation in the pectoral cavity, in the province of Liaoning, China, during October 2018-April 2019. Diseased crabs moved sluggishly, exhibited appetite loss and readily lost legs. Under the microscopic analysis, it was observed that the milky liquid contained a large number of yeastlike microorganisms (0.

First case of non-O1/O139 Vibrio cholerae infection in the pink-tailed chalceus Chalceus macrolepidotus in China.

A pathogen was isolated from diseased pink-tailed chalceus Chalceus macrolepidotus during a high-mortality outbreak in a freshwater culture farm in Liaoyang, China. The diseased fish were characterized by disoriented behaviors, exophthalmos, and redness and swelling of the top of the head. A Gram-negative, pure strain of bacteria (CM0428) was isolated from the brain, kidney, and liver.

Delineating the key virulence factors and intraspecies divergence of via whole-genome sequencing.

is one of the major pathogens in aquaculture. To identify the key virulence factors affecting pathogenesis of towards fish, we conducted a field investigation for three representative fish farms infected with . Multilocus sequence typing (MLST) and whole-genome sequencing were conducted to delineate the phylogenetic relationship and genetic divergence of .

A novel research on isolation and characterization of Photobacterium damselae subsp. damselae from Pacific white shrimp, Penaeus vannamei, displaying black gill disease cultured in China.

In June 2019, massive mortalities of cultured Penaeus vannamei occurred in a local farm in Hainan Province, China. The diseased shrimp displayed evident black gills. Three bacterial strains 20190611001, 20190611007 and 20190611022 were isolated from hepatopancreas and gills of the diseased shrimp and identified as Photobacterium damselae subsp.

Long-distance transmission of pathogenic Vibrio species by migratory waterbirds: a potential threat to the public health.

A potential mechanism for the global distribution of waterborne pathogens is through carriage by the migratory waterbirds. However, this mode of transmission has yet been confirmed epidemiologically. Here, we conducted whole genome sequencing of Vibrio spp.

Mitochondrial genome sequencing of the monogenean isolated from the tiger puffer in North China.

In this study, monogenean was isolated and identified from the gill of diseased Tiger puffer () at an industrial farm in Liaoning, North China (121.3459 E, 38.9861 N).

A Human Intestinal Infection Caused by a Novel Non-O1/O139 Genotype and Its Dissemination Along the River.

Non-O1/O139 is increasingly reported in the clinical settings. However, intestinal infections via the consumption of non-O1/O139 -carrying seafood are rarely documented in China. In this study, we reported a case of mild watery diarrhea in a young male, caused by non-O1/O139 in the downstream of Liaohe River.

Delineating the origins of the multidrug-resistant pathogens in ornamental fish farms by multilocus sequence typing and identification of a novel multidrug-resistant plasmid.

To evaluate the overuse of antibiotics and to identify the origin of pathogens in the ornamental fish industry, we conducted a field investigation of three representative fish farms in Liaoning province, China. Drug-resistant pathogens in the fishponds and groundwater were isolated and subtyped by multilocus sequence typing (MLST). In total, 33 pathogenic strains, including and five other pathogens, were isolated from diseased fish and from groundwater.

Mitochondrial genome sequencing of notorious scuticociliates () isolated from Turbot ( L.).

Previously, a pathogenic ciliate was isolated from the surface ulcer of a diseased Turbot ( L.) at an aquaculture farm in North China. After morphological and molecular biological identification based on 18rRNA, the ciliated was identified as the notorious scuticociliates ().

Localization and characterization of hematopoietic tissues in adult sea cucumber, Apostichopus japonicus.

Sea cucumber Apostichopus japonicus rely on the efficient innate immune mechanisms against invaders, in which the consumption and regeneration of coelomocytes take place at the same time. In the present study, histological features of putative hematopoietic tissues (HPTs) including the rete mirabile, the respiratory tree, the polian vesicle and the coelomic epithelium were characterized. The distribution of transcription factor GATA1 in coelomocytes and putative HPTs was examined by immunohistochemistry.

Mitochondrial genome sequencing and analysis of scuticociliates () isolated from .

Scuticociliates are dangerous parasitic pathogens for in worldwide mariculture. Scuticociliates cause high mortality to marine fish. After an outbreak of scuticociliatosis in in Liaoning Province, northern China, , a scuticociliate, was identified.

Regeneration of coelomocytes after evisceration in the sea cucumber, Apostichopus japonicus.

Sea cucumber, Apostichopus japonicus, is one of the most important holothurian species cultured in China. Severe evisceration induced by various natural and artificial factors commonly occurs during transport and culture of A. japonicus.

Development of a Colloidal Gold-Based Immunochromatographic Assay for the Rapid Detection of .

Edwardsiella Ictaluri is known as the etiological agent of enteric septicaemia of channel catfish, causing heavy economic losses in the aquaculture industry. In this study, a colloidal gold-based immunochromatography assay (GICA) was developed for rapid detection of E. ictaluri.

First characterization of three cyclophilin family proteins in the oyster, Crassostrea ariakensis Gould.

Cyclophilins (CyPs) are a family of proteins that bind the immunosuppressive agent cyclosporin A (CsA) with high-affinity and belong to one of the three superfamilies of peptidyl-prolyl cis-trans isomerases (PPIase). In this report, three cyclophilin genes (Ca-CyPs), including Ca-CyPA, Ca-CyPB and Ca-PPIL3, were identified from oyster, Crassostrea ariakensis Gould in which Ca-CyPA encodes a protein with 165 amino acid sequences, Ca-CyPB encodes a protein with 217 amino acid sequences and Ca-PPIL3 encodes a protein with 162 amino acid sequences. All of the three Ca-CyPs genes contain a typical CyP-PPIase domain with its signature sequences and Ca-CyPB contains an N-signal peptide sequences.

Characterization and expression analysis of a caspase-2 in an invertebrate echinoderm sea cumber Apostichopus japonicus.

Caspase-2 is the most evolutionarily conserved member of the caspase family which mediates the programmed cell death and plays crucial roles in key cellular processes. In this study, a caspase-2 homolog was identified and functionally characterized in sea cucumber Apostichopus japonicus, which we named AjCASP. The full-length cDNA consists of 2100 bp with an ORF encoding a protein of 378 amino acids.

Enhanced immune response and resistance to edwardsiellosis following dietary chitooligosaccharide supplementation in the olive flounder (Paralichthys olivaceus).

This study was conducted to evaluate the effects of dietary chitooligosaccharide (COS) supplementation on peripheral leukocyte count, head kidney leukocyte phagocytic rate, phagocytic index, respiratory burst activity, serum lysozyme activity, and immune protection in Paralichthys olivaceus. A total of 300 flounder with an average body weight of 80-100 g were randomly assigned into four dietary groups: (I) basic diet (control), basic diet containing (II) 0.5% COS, and (III) 1% COS, fed continuously for 28 d, and (IV) basic diet containing 1% COS fed in 14 d intervals.

Ontogenesis of coelomocytes in sea cucumber (Apostichopus japonicus) studied with probes of monoclonal antibody.

Monoclonal antibodies (MAbs) specifically against coelomocytes of Apostichopus japonicus were employed to study the ontogenesis of coelomocytes by indirect immunofluorescence assay technique (IIFAT). Different developmental stages were identified by histochemical staining method. Stages including blastula, gastrula, auricularia (small-auricular larvae, mid-auricular larvae and big-auricular larvae), doliolaria, pentactula and juvenile were examined.

Comparison of cells free in coelomic and water-vascular system of sea cucumber, Apostichopus japonicus.

The sea cucumber, Apostichopus japonicus possesses a variety of cells populating in both the coelomic (cells in the coelomic are called coelomocytes) and water-vascular system. In this study, we compared cells in these two systems of A. japonicus on total cell number, cell types and surface antigens through monoclonal antibodies against coelomocytes.