Antimicrobial activity of α/β hybrid peptides incorporating tBu-βAcc against methicillin-resistant Staphylococcus aureus.

The incorporation of β-amino acids into peptides is a promising approach to develop proteolytically stable therapeutic agents. Short α/β hybrid peptides containing tBu-βAccː HN-Lys-tBu-βAcc-PEA, P1; HN-Orn-tBu-βAcc-PEA, P2; HN-Arg-tBu-βAcc-PEA, P3; LA-Lys-tBu-βAcc-PEA, P4; LA-Orn-tBu-βAcc-PEA, P5; LA-Arg-tBu-βAcc-PEA, P6; LA-Lys-tBu-βAcc-PEA, P7; LA-Orn-tBu-βAcc-PEA, P8; and LA-Arg-tBu-βAcc-PEA, P9 were prepared. The antimicrobial efficacies of all the peptides were evaluated against ESKAPE pathogens, along with a small panel of multi-drug resistant (MDR) clinical isolates of S.