Identifying the function of the PI3K-AKT pathway during the pathogenic infection of Macrobrachium rosenbergii.

The phosphoinositide-3-kinase/protein kinase b (PI3K-Akt) pathway is a signalling pathway based on protein phosphorylation and can be activated by a wide range of factors. To investigate the function of the PI3K-AKT signalling pathway in antibacterial immunity, we analysed the gene expression level of three key factors (PI3K, AKT and FoxO) and innate immune factors in immune tissues at different time points after Vibrio parahaemolyticus and Staphylococcus aureus infection. Tissues analysis showed that PI3K, AKT, and FoxO were expressed at high levels in the intestinal, hemocytes and hepatopancreas.

Transcriptome analysis of Macrobrachium rosenbergii hemocytes in response to Staphylococcus aureus infection.

The aquaculture industry has suffered significant financial losses as a result of disease outbreaks. In particular, disease outbreaks have become a major problem that can seriously affect the sustainable development of the Macrobrachium rosenbergii aquaculture industry. It is crucial to determine the defense mechanism of the host after pathogenic invasion in order to provide effective defense measures after disease outbreaks.

Chrysin improves diabetic nephropathy by regulating the AMPK-mediated lipid metabolism in HFD/STZ-induced DN mice.

Diabetic nephropathy (DN) is a highly prevalent and severe diabetic complication. It is urgent to explore high efficiency and minor side effects therapy for DN. Chrysin is a natural flavonoid with various biological activities found in honey and propolis, and has considerable potential to improve DN.

Hydrodynamics Analysis and CFD Simulation of Portal Venous System by TIPS and LS.

In cirrhotic patients, portal hypertension is often associated with a hyperdynamic changes. Transjugular Intrahepatic Portosystemic Shunt (TIPS) and Laparoscopic splenectomy are both treatments for liver cirrhosis due to portal hypertension. While, the two different interventions have different effects on hemodynamics after operation and the possibilities of triggering PVT are different.

Determination of eight pharmaceuticals in an aqueous sample using automated derivatization solid-phase microextraction combined with gas chromatography-mass spectrometry.

An automated aqueous derivatization solid-phase microextraction (SPME) coupled with a gas chromatography/mass spectrometry (GC/MS) method was developed for simultaneous determination of eight pharmaceuticals in water samples. Dimethyl sulfate and tetrabutylammonium hydrogen sulfate were selected as derivatization and activation reagents for the esterification reaction. An experimental design approach, central composition design (CCD), was employed to investigate and optimize the operative factors influencing the extraction efficiency, including extraction time, extraction temperature and ionic strength.

Radiofrequency ablation versus cryosurgery ablation for hepatocellular carcinoma: a meta-analysis.

Background/aims: To investigate the meta-analysis of the effect between radiofrequency ablation (RFA) and cryosurgery ablation (CSA) in treating unresectable hepatocellular carcinoma (HCC).

Methodology: Systematic literature search of relevant clinical studies was carried out in Pubmed, Embase, Google Scholar, the Cochrane Library Central databases and the Chinese National Knowledge Infrastructure databases. Data were ed independently by two reviewers.

Carbon dioxide (CO₂) ablation as a remedy for hepatocellular carcinoma.

Background/aims: The purpose of this research was to investigate the application of carbon dioxide (CO₂) ablation and determine if carbon dioxide plays a role in treating hepatocellular carcinoma.

Methodology: Twelve mice with subcutaneous xenografts of a hepatocellular cancer cell line were randomly separated into 2 groups. One group underwent CO₂ ablation with their liver tumor in vivo while the other group in vitro, respectively.