Improved production of alkaline and solvent-stable proteases from a halotolerant Exiguobacterium isolate through heterologous expression.

Halophiles are excellent sources of detergent proteases that are attributed to stability in alkaline pH, salts, surfactants, and hydrophobic solvents. The lower enzymatic yields and tedious downstream processes necessitate the search for newer halophilic sources. We have previously reported a halotolerant Exiguobacterium sp.

Pan-genomic comparison of a potential solvent-tolerant alkaline protease-producing sp. TBG-PICH-001 isolated from a marine habitat.

Unlabelled: The identification and applicability of bacteria are inconclusive until comprehended with genomic repositories. Our isolate, sp. TBG-PICH-001 exhibited excellent halo- and organic solvent tolerance with simultaneous production of alkaline protease/s (0.

Evaluation of antibiofilm potential of four-domain α-amylase from Streptomyces griseus against exopolysaccharides (EPS) of bacterial pathogens using Danio rerio.

Biofilm formation is a major issue in healthcare settings as 75% of nosocomial infection arises due to biofilm residing bacteria. Exopolysaccharides (EPS), a key component of the biofilm matrix, contribute to the persistence of cells in a complex milieu and defends greatly from exogenous stress and demolition. It has been shown to be vital for biofilm scaffold and pathogenic features.

A highly divergent α-amylase from Streptomyces spp.: An evolutionary perspective.

The present study deals with the genetic changes observed in the protein sequence of an α-amylase from Streptomyces spp. and its structural homologs from Pseudoalteromonas haloplanktis, invertebrates and mammals. The structural homologs are renowned for their important features such as chloride binding triad and a serine-protease like catalytic triad (a triad which is reported to be strictly conserved in all chloride-dependent α-amylases).

Cloning, expression, homology modelling and molecular dynamics simulation of four domain-containing α-amylase from Streptomyces griseus.

In the present study, α-amylase from TBG19NRA1 was amplified, cloned and successfully expressed in BL21/DE3. Sequence analysis of α-amylase (SGAmy) revealed the presence of four domains (A, B, C and E). Alpha-amylases with E domain (also known as carbohydrate binding module 20 (CBM20)) are capable of degrading raw starch and this property holds great potential for application in starch processing industries.

Fukugiside, a biflavonoid from Garcinia travancorica inhibits biofilm formation of Streptococcus pyogenes and its associated virulence factors.

Purpose: Streptococcus pyogenes, a notorious human pathogen thatis responsible for various invasive and non-invasive diseases, possesses multiple virulence armaments, including biofilm formation. The current study demonstrates the anti-biofilm and anti-virulence potential of fukugiside, a biflavonoid isolated from Garciniatravancorica, against S. pyogenes.

A S52P mutation in the 'α-crystallin domain' of Mycobacterium leprae HSP18 reduces its oligomeric size and chaperone function.

Mycobacterium leprae HSP18 is a small heat shock protein (sHSP). It is a major immunodominant antigen of M. leprae pathogen.

Characterization and phylogenetic analysis of cellulase producing Streptomyces noboritoensis SPKC1.

A cellulase producing strain of Actinomycetes was isolated from soil samples which were collected from Southwest ghats, Kerala, India at a depth of 6-12 inches and Actinomycetes was characterized by morphological, cultural, physiological, chemotaxonomical and phylogenetic analysis. The 16S rRNA region of this strain was amplified and sequenced. The Neighbor-joining and Maximum Parsimony algorithm with topology tree of 16S rRNA was constructed.

Functional characterization of a small heat shock protein from Mycobacterium leprae.

Background: Small heat shock proteins are ubiquitous family of stress proteins, having a role in virulence and survival of the pathogen. M. leprae, the causative agent of leprosy is an uncultivable organism in defined media, hence the biology and function of proteins were examined by cloning M.

Chemical composition and antimicrobial activity of essential oil from the rhizomes of Amomum cannicarpum.

The essential oil obtained by hydrodistillation of the rhizomes of Amomum cannicarpum was analyzed by GC and GC/MS. Thirty-four compounds were identified, of which the major components were beta-terpineol (13.4%), beta-pinene (9.

Chemical composition and antibacterial activity of the rhizome oil of Hedychium larsenii.

The composition of essential oil from the rhizomes of Hedychium larsenii M. Dan & Sathish was examined by GC-FID and GC-MS techniques. 99% of the oil consisted of monoterpenoids.

Antibacterial effects of Coscinium fenestratum.

Antibacterial activity of Coscinium fenestratum is mainly due to the presence of berberine.

Antimicrobial activity of Amomum cannicarpum.

The petroleum ether and methanol extracts of rhizomes of Amomum cannicarpum exhibited moderate inhibiting activity against both Gram-positive and Gram-negative bacteria. None of the extractives was active against the tested moulds.